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            <gco:CharacterString>An exploratory project on the taxonomic and genetic diversity of decapods in three ocean subregions (Northeast Pacific, Canadian Arctic, and Northwest Atlantic), which were sampled in 2022, was undertaken by the Arctic Working Group under the Canada-U.S. Fisheries and Climate Collaboration between Fisheries and Oceans Canada (DFO) and the National Marine Fisheries Service (NMFS) of the National Oceanic and Atmospheric Administration (NOAA). This collaboration framework aims to pool Canadian and U.S. data to explore the impacts of broad-scale climate change on marine biodiversity. In early summer 2022, a sampling protocol with the selection of targeted decapods was provided to DFO and NOAA collaborators. Targeted genera were collected from a total of 10 research programs across three ocean subregions and four marine regions. 

The Northeast Pacific samples were collected in the Bering Sea during the Northern Bering Sea Ecosystem and Surface Trawl Survey, and the Eastern and Northern Bering Sea Continental Shelf Bottom Trawl Survey of Groundfish and Invertebrate Fauna onboard the F/V Northwest Explorer, F/V Alaska Knight and F/V Vesteraalen. In the Western Canadian Arctic (mainly from Beaufort Sea and Amundsen Gulf), specimens were collected during DFO’s Canadian Beaufort Sea – Marine Ecosystem Assessment (CBS-MEA) survey onboard the F/V Frosti. 

In Eastern Canadian Arctic (mainly from Baffin Bay and Davis Strait), specimens were collected during DFO’s Knowledge and Ecosystem-Based Approach in Baffin Bay (KEBABB) survey onboard the CCGS Amundsen and DFO’s North Atlantic Fisheries Organization (NAFO) Subarea 0B survey onboard the R/V Tarajoq. 

In the Estuary and Gulf of St. Lawrence (EGSL), specimens were collected from coastal surveys (scallops, sea cucumber, snow crab, and whelk surveys) onboard the CCGS Leim and offshore during the Ecosystemic Survey onboard the CCGS Teleost. Decapods were collected from various sampling gears (benthic beam trawl, modified Atlantic Western IIA otter trawl, Bacalao trawl, shrimp trawl, Digby scallop dredge, or modified sea cucumber dredge) and identified to the lowest possible taxonomic level and photographed, when possible. All specimens were frozen at sea (n = 995). In the lab, the identifications were validated or refined with the photos and the frozen specimens. DNA was extracted for 87 specimens and a section of COI gene was amplified in order to be sequenced using Sanger method. Sequences were compared with existing data using The Basic Local Alignment Search Tool (BLAST) in the National Center for Bio-technology Information Nucleotide database (NCBI-nt, including the GenBank database) to compare scientific names, where available.

The present dataset includes 391 decapod species occurrences. DNA was extracted for a subset of 87 specimens (COI gene); sequences are publicly available on BOLD data portal under project code DDAO (see supporting document "citations_references.csv" for more information).

The data are presented in Darwin Core format and are separated in three files:
The "Activité_décapodes_DDAO_decapods_event_en" file contains information about missions, stations and deployments, which are presented under a hierarchical activity structure.
The "Occurrence_décapodes_DDAO_decapods_en" file contains the taxonomic occurrences.
The "ADN_décapodes_DDAO_decapods_DNA_en" file contains the DNA derived data.

For further details, please refer to the technical report available in the supporting document named "citations_references.csv".
            

USE LIMITATION:
To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.</gco:CharacterString>
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                  <gmd:LocalisedCharacterString locale="#fra">Un projet exploratoire sur la diversité taxonomique et génétique de décapodes échantillonnés en 2022 dans trois sous-régions océaniques (Pacifique Nord-Est, Arctique canadien et Atlantique Nord-Ouest) a été réalisé par le groupe de travail sur l'Arctique au sein de la collaboration canado-américaine sur les pêches et le climat entre Pêches et Océans Canada (MPO) et le National Marine Fisheries Service (NMFS) de la National Oceanic and Atmospheric Administration (NOAA). Ce cadre de collaboration vise à mettre en commun les données canadiennes et américaines afin d'étudier les effets du changement climatique à grande échelle sur la biodiversité marine. Au début de l'été 2022, un protocole d'échantillonnage illustrant une sélection des décapodes ciblés a été fourni aux collaborateurs du MPO et de la NOAA. Les genres ciblés ont été collectés dans le cadre de 10 programmes de recherche au total, dans trois sous-régions océaniques et quatre régions marines. 

Les échantillons du Pacifique Nord-Est ont été collectés dans la mer de Béring au cours de l'étude de l'écosystème de la mer de Béring septentrionale et du chalutage de surface, et de l'étude du chalutage de fond du plateau continental de la mer de Béring orientale et septentrionale sur les poissons de fond et la faune invertébrée, à bord du F/V Northwest Explorer, du F/V Alaska Knight et du F/V Vesteraalen. Dans l'ouest de l'Arctique canadien (principalement dans la mer de Beaufort et le golfe d'Amundsen), les spécimens ont été collectés au cours de l'étude du MPO sur l'évaluation de l'écosystème marin de la mer de Beaufort (CBS-MEA) à bord du F/V Frosti. 

Dans l'est de l'Arctique canadien (principalement dans la baie de Baffin et le détroit de Davis), les spécimens ont été recueillis lors du relevé de l'approche fondée sur les connaissances et les écosystèmes dans la baie de Baffin (KEBABB) du MPO à bord du NGCC Amundsen et du relevé de la sous-zone 0B de l'Organisation des pêches de l'Atlantique Nord (OPANO) à bord du R/V Tarajoq. 

Dans l'estuaire et le golfe du Saint-Laurent (EGSL), des spécimens ont été recueillis lors de relevés côtiers (relevés sur le buccin, le concombre de mer, le crabe des neiges et les pétoncles) à bord du NGCC Leim et au large lors du relevé écosystémique à bord du NGCC Teleost. Les décapodes ont été collectés à partir de divers engins d'échantillonnage (chalut à perche benthique, chalut à panneaux modifié Atlantic Western IIA, chalut Bacalao, chalut à crevettes, drague à pétoncles de type Digby ou drague à concombres de mer modifiée) et identifiés au niveau taxonomique le plus bas possible et photographiés, dans la mesure du possible. Tous les spécimens ont été congelés en mer (n = 995). Au laboratoire, les identifications ont été validées ou affinées à l'aide des photos et des spécimens congelés. L'ADN de 87 spécimens a été extrait et une section du gène COI a été amplifiée afin d'être séquencée à l'aide de la méthode Sanger. Les séquences ont été comparées aux données existantes à l'aide de l'outil BLAST (Basic Local Alignment Search Tool) dans la base de données des nucléotides du National Center for Bio-technology Information (NCBI-nt, y compris la base de données GenBank) afin de comparer les noms scientifiques, le cas échéant.

L'ensemble de données actuel comprend 391 occurrences d'espèces de décapodes. L'ADN a été extrait d'un sous-groupe de 87 spécimens (gène COI). Les séquences sont accessibles publiquement sur la plateforme BOLD sous le code de projet DDAO (voir le document de soutien « citations_references.csv » pour plus d'informations).

Les données sont présentées en format Darwin Core et sont séparées en trois fichiers :
Le fichier " Activité_décapodes_DDAO_decapods_event_fr" contient les informations des missions, des stations et des déploiements, qui sont présenté sous une structure d'activité hiérarchique.
Le fichier " Occurrence_décapodes_DDAO_decapods_fr" contient les occurrences taxonomiques.
Le fichier "ADN_décapodes_DDAO_decapods_DNA_fr" contient les données ADN associées.

Pour de plus amples détails, veuillez vous référer au rapport technique disponible dans le document de soutien intitulé « citations_references.csv ».
            
LIMITATION DE L'UTILISATION :
Pour assurer l'intégrité scientifique et l'utilisation appropriée des données, nous vous encourageons à contacter le gardien des données.</gmd:LocalisedCharacterString>
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