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            <gco:CharacterString>Marine Protected Areas require comprehensive monitoring to ensure objectives are achieved; however, monitoring natural ecosystems at scale is challenged by the biodiversity it aims to measure. Environmental DNA (eDNA) metabarcoding holds promise to address this monitoring challenge. We conducted paired sampling at 54 sites for fish and invertebrate assemblages in the Northwest Atlantic using groundfish trawls and eDNA metabarcoding of benthic seawater using four genetic markers (12S rRNA, 16S rRNA, 18S rRNA, and CO1). Compared to trawling, eDNA detected similar patterns of species turnover, larger estimates of gamma diversity, and smaller estimates of alpha diversity. A total of 63.6% (42/66) of fish species captured by trawling were detected by eDNA, along with an additional 26 species. Of the 24 missed detections by eDNA, 12 were inevitable as they lacked reference sequences. Excluding taxa assigned to higher than species level and those without a species name, 23.6% (17/72) of invertebrate species captured by trawling were detected by CO1, which detected an additional 98 species. We demonstrate that eDNA is capable of detecting patterns of community assemblage and species turnover in an offshore environment, emphasizing its strong potential for a non-invasive, comprehensive, and scalable tool for biodiversity monitoring supporting marine conservation programmes.

Cite this data as: Jeffery, N., Rubidge, E., Abbott, C., Westfall, K., Stanley, R. (2024): Data of: eDNA metabarcoding enriches traditional trawl survey data for monitoring biodiversity in the marine environment.

Published: August 2024. Coastal Ecosystems Science Division, Fisheries and Oceans Canada, Dartmouth, N.S. https://open.canada.ca/data/en/dataset/43a91ba7-8025-4330-88db-db14022d729d</gco:CharacterString>
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                  <gmd:LocalisedCharacterString locale="#fra">Les zones de protection marine doivent faire l’objet d’un suivi complet pour garantir la réalisation de leurs objectifs. Toutefois, le suivi des écosystèmes naturels à grande échelle est compliqué par la biodiversité qu’il vise à mesurer. Le métacodage à barres de l’ADN environnemental (ADNe) est une solution prometteuse pour relever ce défi de surveillance. Nous avons procédé à un échantillonnage jumelé sur 54 sites pour les assemblages de poissons et d’invertébrés dans l’Atlantique nord-ouest en utilisant des chaluts à poissons de fond et un métacodage à barres de l’ADNe de l’eau de mer benthique en utilisant quatre marqueurs génétiques (ARNr 12S, ARNr 16S, ARNr 18S, et CO1). Par rapport au chalutage, l’ADNe a permis de détecter des schémas similaires de renouvellement des espèces, des estimations plus importantes de la diversité gamma et des estimations plus faibles de la diversité alpha. Au total, 63,6 % (42/66) des espèces de poissons capturées par chalutage ont été détectées par l’ADNe, ainsi que 26 espèces supplémentaires. Sur les 24 détections manquées par l’ADNe, 12 étaient inévitables, car elles manquaient de séquences de référence. Si l’on exclut les taxons classés à un niveau supérieur à celui de l’espèce et ceux qui n’ont pas de nom d’espèce, 23,6 % (17/72) des espèces d’invertébrés capturées par le chalutage ont été détectées par CO1, qui a détecté 98 espèces supplémentaires. Nous démontrons que l’ADNe est capable de détecter des schémas d’assemblage de communautés et de renouvellement des espèces dans un environnement extracôtier en soulignant son fort potentiel en tant qu’outil non invasif, complet et évolutif pour la surveillance de la biodiversité qui soutient les programmes de conservation marine.

Citer ces données comme suit: Jeffery, N., Rubidge, E., Abbott, C., Westfall, K., Stanley, R. (2024).

Données de Le métacodage à barres de l’ADNe enrichit les données des relevés au chalut traditionnels pour le suivi de la biodiversité dans l’environnement marin. Date de publication Août 2024. Division de la science des écosystèmes côtiers, Pêches et Océans Canada, Dartmouth, (N-É).

https://open.canada.ca/data/en/dataset/43a91ba7-8025-4330-88db-db14022d729d</gmd:LocalisedCharacterString>
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      </gmd:MD_Distribution>
  </gmd:distributionInfo>
</gmd:MD_Metadata>