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            <gco:CharacterString>PURPOSE:
To provide access to detailed stomach content data and associated metadata from Atlantic Bluefin Tuna sampled in the southern Gulf of St. Lawrence from 2018 to 2023. These data support fisheries science by contributing to analyses of predator–prey dynamics, diet composition, and ecosystem understanding, as well as informing stock assessment and fisheries management activities within Fisheries and Oceans Canada.
            
DESCRIPTION:
This dataset contains metadata and stomach content information collected from Atlantic Bluefin Tuna (ABFT) caught from mid-August to late September in the commercial fishery in the southern Gulf of St. Lawrence between 2018 and 2023. Stomach samples were primarily obtained from fish harvested near the eastern end of Prince Edward Island, with additional samples collected from the Miscou/Baie‑des‑Chaleurs area in 2018 and 2019.

SAMPLING METHODS:
Fish were measured to the nearest curved fork length (cm) and weighed to the nearest round weight (kg). Stomachs were obtained directly from harvesters or through a fish buyer and were stored at −20 ◦C before being processed in the laboratory. Stomachs identification numbers were cross-referenced with ABFT tag numbers recorded by fish provider in order to obtain logbook and port data (catch location, time, weight length, sex, gear, etc.) for each sample. Stomachs were thawed in the laboratory and the content was sorted and identified to the lowest possible taxonomic level. For each stomach, prey were weighed collectively as a taxonomic group and individually to the nearest 0.1 g. 

Dead bait used to capture ABFT, identified by cut marks, were recorded and weighed but excluded from the analysis. Live bait items cannot be identified from stomach content analyses. Only a few otoliths were found in 2018 and their degraded quality precluded performing ageing or species identification. Rare and small prey items such as algae and rocks were classified in the category “other”. Fish remains that could not be identified were classified in the category “Unidentified teleostei remains”.

For 2019 to 2023, when stomach content items could not be visually identified and when tissue was available, tissue samples were collected and stored at −20 °C for DNA barcoding analysis. DNA extraction, mitochondrial cytochrome oxidase subunit 1 amplification, Sanger sequencing and species assignation were performed at the Plateforme d’Analyses Génomiques and Plateforme Bio-informatique of the Institut de Biologie Intégrative et des Systèmes (PAG-IBIS, Université Laval, Quebec city, QC, Canada, http://www.ibis.ulaval.ca/en/services-2/genomic-analysis-platform/). 

DNA was extracted from 20 mg of muscle tissue using the Omega Bio-tek E-Z-96 Tissue DNA Kit (Omega Bio-tek, Norcross GA, USA) following manufacturer instructions. The mitochondrial cytochrome oxidase subunit 1 region was amplified and sequenced as described in Hashemzadeh Segherloo et al., 2021). Sanger forward and reverse reads were analyzed independently using the Basic Local Alignment Search Tool against non-redundant sequences to identify the top hit for each sequence. When samples could not be identified by a top hit sequence they were classified as “unidentifiable fish”. 

Prey items that were successfully identified using DNA barcoding were incorporated into the stomach content analysis database and used in all subsequent diet analyses (abundance, occurrence and weight). The weight of the items used in the database was the weight of the remains as they were, and not reconstructed weights calculated for a live animal of the species identified by the barcoding.

USE LIMITATION:
To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.</gco:CharacterString>
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                  <gmd:LocalisedCharacterString locale="#fra">OBJECTIF :
Fournir l’accès à des données détaillées sur le contenu stomacal et aux métadonnées associées du thon rouge de l’Atlantique échantillonné dans le sud du golfe du Saint‑Laurent entre 2018 et 2023. Ces données appuient les sciences halieutiques en contribuant aux analyses des dynamiques prédateur‑proie, de la composition du régime alimentaire et de la compréhension de l’écosystème, ainsi qu’à l’évaluation des stocks et aux activités de gestion des pêches au sein de Pêches et Océans Canada.
            
DESCRIPTION :
Ce jeu de données contient des métadonnées et des informations sur le contenu stomacal recueillies chez le thon rouge de l’Atlantique (ABFT) capturé de la mi‑août à la fin septembre dans la pêche commerciale dans le sud du golfe du Saint‑Laurent entre 2018 et 2023. Les échantillons d’estomac proviennent principalement de poissons récoltés près de l’extrémité est de l’Île‑du‑Prince‑Édouard, avec des échantillons supplémentaires recueillis dans la région de Miscou/Baie‑des‑Chaleurs en 2018 et 2019.
            
MÉTHODES D'ÉCHANTILLONNAGE :
Les poissons ont été mesurés à la longueur courbée à la fourche la plus proche (cm) et pesés au poids rond le plus proche (kg). Les estomacs ont été obtenus directement auprès des pêcheurs ou par l’intermédiaire d’un acheteur de poisson, puis stockés à −20 °C avant d’être analysés en laboratoire. Les numéros d’identification des estomacs ont été croisés avec les numéros de marque des thons rouges enregistrés par le fournisseur afin d’obtenir les données des journaux de bord et des ports (lieu et moment de capture, poids, longueur, sexe, engin, etc.) pour chaque échantillon. Les estomacs ont été décongelés en laboratoire, puis leur contenu a été trié et identifié au niveau taxonomique le plus bas possible. Pour chaque estomac, les proies ont été pesées collectivement par groupe taxonomique et individuellement au 0,1 g près.

Les appâts morts utilisés pour capturer les thons rouges, identifiés par des marques de coupe, ont été enregistrés et pesés mais exclus de l’analyse. Les appâts vivants ne peuvent pas être identifiés par l’analyse du contenu stomacal. Seuls quelques otolithes ont été trouvés en 2018, et leur qualité dégradée n’a pas permis d’effectuer une détermination de l’âge ou de l’espèce. Les proies rares et de petite taille, telles que les algues et les roches, ont été classées dans la catégorie « autres ». Les restes de poissons qui n’ont pas pu être identifiés ont été classés dans la catégorie « restes de téléostéens non identifiés ».

De 2019 à 2021, lorsque les éléments du contenu stomacal ne pouvaient pas être identifiés visuellement et lorsque des tissus étaient disponibles, des échantillons de tissus ont été prélevés et stockés à −20 °C pour une analyse par code-barres ADN. L'extraction de l'ADN, l'amplification de la sous-unité 1 de la cytochrome oxydase mitochondriale, le séquençage Sanger et l'attribution des espèces ont été réalisés à la Plateforme d’Analyses Génomiques et à la Plateforme Bio-informatique de l'Institut de Biologie Intégrative et des Systèmes (PAG-IBIS, Université Laval, Québec, QC, Canada, http://www.ibis.ulaval.ca/en/services-2/genomic-analysis-platform/).

L'ADN a été extrait à partir de 20 mg de tissu musculaire à l'aide du kit Omega Bio-tek E-Z-96 Tissue DNA (Omega Bio-tek, Norcross, GA, USA) en suivant les instructions du fabricant. La région de la sous-unité 1 de la cytochrome oxydase mitochondriale a été amplifiée et séquencée comme décrit dans Hashemzadeh Segherloo et al. (2021). Les séquences Sanger, en lecture avant et arrière, ont été analysées indépendamment à l’aide de l’outil Basic Local Alignment Search Tool contre les séquences non redondantes afin d’identifier la meilleure correspondance pour chaque séquence. Lorsque les échantillons ne pouvaient pas être identifiés par une correspondance de séquence, ils étaient classés comme « poissons non identifiables ».

Les proies identifiées avec succès grâce au code-barres ADN ont été intégrées dans la base de données d'analyse du contenu stomacal et utilisées dans toutes les analyses ultérieures du régime alimentaire (abondance, occurrence et poids). Le poids des éléments enregistrés dans la base de données correspondait au poids des restes tels qu’ils étaient et non à des poids reconstruits estimés pour un animal vivant de l’espèce identifiée par le code-barres ADN.

LIMITATION DE L'UTILISATION :
Pour assurer l'intégrité scientifique et l'utilisation appropriée des données, nous vous encourageons à contacter le gardien des données.</gmd:LocalisedCharacterString>
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                                       <gmd:protocol>
                                          <gco:CharacterString>WWW:LINK-1.0-http--link</gco:CharacterString>
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                              <gmd:CI_DateTypeCode codeList="https://schemas.metadata.geo.ca/register/napMetadataRegister.xml#IC_87"
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                        <gmd:CI_Date>
                           <gmd:date>
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                              <gmd:CI_DateTypeCode codeList="https://schemas.metadata.geo.ca/register/napMetadataRegister.xml#IC_87"
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                           <gmd:code>
                              <gmx:Anchor xlink:href="https://api-proxy.edh-cde.dfo-mpo.gc.ca/catalogue/registries/vocabularies/external.theme.GC_Core_Subject">geonetwork.thesaurus.external.theme.GC_Core_Subject</gmx:Anchor>
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                        </gmd:MD_Identifier>
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                              <gmd:CI_RoleCode codeList="https://schemas.metadata.geo.ca/register/napMetadataRegister.xml#IC_90"
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               <gmd:thesaurusName>
                  <gmd:CI_Citation>
                     <gmd:title xsi:type="gmd:PT_FreeText_PropertyType">
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                        <gmd:CI_Date>
                           <gmd:date>
                              <gco:Date>2009-01-06</gco:Date>
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                           <gmd:dateType>
                              <gmd:CI_DateTypeCode codeList="http://nap.geogratis.gc.ca/metadata/register/napMetadataRegister.xml#IC_87"
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                     <gmd:date>
                        <gmd:CI_Date>
                           <gmd:date>
                              <gco:Date>2009</gco:Date>
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                           <gmd:dateType>
                              <gmd:CI_DateTypeCode codeList="http://nap.geogratis.gc.ca/metadata/register/napMetadataRegister.xml#IC_87"
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                        <gmd:CI_Date>
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                              <gco:Date>2009</gco:Date>
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                              <gmd:CI_DateTypeCode codeList="http://nap.geogratis.gc.ca/metadata/register/napMetadataRegister.xml#IC_87"
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                                          <gco:CharacterString>WWW:LINK-1.0-http--link</gco:CharacterString>
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                     <gmd:name xsi:type="gmd:PT_FreeText_PropertyType">
                        <gco:CharacterString>Supplementary citation list</gco:CharacterString>
                        <gmd:PT_FreeText>
                           <gmd:textGroup>
                              <gmd:LocalisedCharacterString locale="#fra">Liste de citations supplémentaires</gmd:LocalisedCharacterString>
                           </gmd:textGroup>
                        </gmd:PT_FreeText>
                     </gmd:name>
                     <gmd:description xsi:type="gmd:PT_FreeText_PropertyType">
                        <gco:CharacterString>Supporting Document;PDF;eng,fra</gco:CharacterString>
                        <gmd:PT_FreeText>
                           <gmd:textGroup>
                              <gmd:LocalisedCharacterString locale="#fra">Document de soutien;PDF;eng,fra</gmd:LocalisedCharacterString>
                           </gmd:textGroup>
                        </gmd:PT_FreeText>
                     </gmd:description>
                  </gmd:CI_OnlineResource>
               </gmd:onLine>
            </gmd:MD_DigitalTransferOptions>
         </gmd:transferOptions>
      </gmd:MD_Distribution>
  </gmd:distributionInfo>
</gmd:MD_Metadata>