Across the Canadian North, Arctic Char, Salvelinus alpinus, are culturally important and critical for maintaining subsistence lifestyles and ensuring food security for Inuit. Arctic Char also support economic development initiatives in many Arctic communities through the establishment of coastal and inland commercial char fisheries. The Halokvik River, located near the community of Cambridge Bay, Nunavut, has supported a commercial fishery for anadromous Arctic Char since the late 1960s. The sustainable management of this fishery, however, remains challenging given the lack of biological data on Arctic Char from this system and the limited information on abundance and biomass needed for resolving sustainable rates of exploitation. In 2013 and 2014, we enumerated the upstream run of Arctic Char in this system using a weir normally used for commercial harvesting. Additionally, we measured fish length and used T-bar anchor tags to mark a subset of the run. Subsequently, we estimated population size using capture-mark-recapture (CMR) methods. The estimated number of Arctic Char differed substantially between years. In 2013, 1967 Arctic Char were enumerated whereas in 2014, 14,502 Arctic Char were enumerated. We attribute this marked difference primarily to differences in weir design between years. There was also no significant relationship between daily mean water temperature and number of Arctic Char counted per day in either year of the enumeration. The CMR population estimates of Arctic Char (those ≥450mm in length) for 2013 and 2014 were 35,546 (95% C.I 30,513-49,254) and 48,377 (95% C.I. 37,398-74,601) respectively. The 95% CI overlapped between years, suggesting that inter-annual differences may not be as extreme as what is suggested by the enumeration. The population estimates reported here are also the first estimates of population size for an Arctic Char stock in the Cambridge Bay region using CMR methodology. Overall, the results of this study will be valuable for understanding how population size may fluctuate over time in the region and for potentially providing advice on the sustainable rates of harvest for Halokvik River Arctic Char. Additionally, the results generated here may prove valuable for validating current stock assessment models that are being explored for estimating biomass and abundance for commercial stocks of Arctic Char in the region.