Evaluating an Autonomous eDNA Sampler for Marine Environmental Monitoring: Short- and Long-Term Applications

We evaluated an autonomous environmental DNA sampler produced by Dartmouth Ocean Technologies Inc (Dartmouth, Canada) compared to time-at-sample filtration in the laboratory to determine the performance of moored samplers for monitoring in the marine world. We deployed three autonomous samplers from DOT in the Bedford Basin (Canada) over a nine-week period in summer/fall 2023. The samplers filtered seawater in situ at programmed interviews over this time period, and we collected contemporaneous samples with a standard vacuum pump during each sampling period. Both eDNA sample types captured similar fish diversity, including typical diversity for the Northwest Atlantic. The invertebrate community detected using the COI marker was different between each sample type, likely due to differences in filter pore size. We found biofouling on the moored samplers was minimal over the study period, even in a high-traffic area such as the Bedford Basin, likely due to the relatively short experimental period, and copper screening covering in the inlet and outlet valves of the instruments. Overall, our results show promise to deploy autonomous eDNA samplers in marine conservation areas to contribute to monitoring in the temperate ocean, but further testing over longer periods of time is needed to determine if DNA remains well-preserved in the autonomous samplers at ambient ocean temperatures.

Cite this data as: Jeffery, N.W., Van Wyngaarden, M., and Stanley, R.R.E. Evaluating an Autonomous eDNA Sampler for Marine Environmental Monitoring: Short- and Long-Term Applications. Published: December 2024. Coastal Ecosystems Science Division, Maritimes Region, Fisheries and Oceans Canada, Dartmouth NS.

Simple

Date ( RI_367 )
2024-11-29
Date ( RI_366 )
2024-10-23
RI_415
  Government of Canada; Fisheries and Oceans Canada; Coastal Ecosystem Science Division (CESD) - Nick Jeffery ( )
Bedford Institute of Oceanography, 1 Challenger Drive, PO Box 1006 , Dartmouth , Nova Scotia , B2Y 4A2 , Canada
RI_419
  Government of Canada; Fisheries and Oceans Canada; Coastal Ecosystem Science Division (CESD) - Mallory Van Wyngaarden ( )
Bedford Institute of Oceanography, 1 Challenger Drive, PO Box 1006 , Dartmouth , Nova Scotia , B2Y 4A2 , Canada
RI_419
  Government of Canada; Fisheries and Oceans Canada; Coastal Ecosystem Science Division (CESD) - Ryan Stanley ( )
Bedford Institute of Oceanography, 1 Challenger Drive, PO Box 1006 , Dartmouth , Nova Scotia , B2Y 4A2 , Canada
Status
completed; complété RI_593
Maintenance and update frequency
notPlanned; nonPlanifié RI_542
Keywords
  • Marine Conservation Target
  • Partnerships
Government of Canada Core Subject Thesaurus Thésaurus des sujets de base du gouvernement du Canada ( RI_528 )
  • Marine conservation areas
  • Oceans
  • Coastal waters
  • Genetics
Canada/US States and Provinces Canada/US États et provinces ( RI_528 )
  • Nova Scotia
Use limitation
Open Government Licence - Canada (http://open.canada.ca/en/open-government-licence-canada)
Access constraints
license; licence RI_606
Use constraints
license; licence RI_606
Classification
unclassified; nonClassifié RI_484
Spatial representation type
vector; vecteur RI_635
Metadata language
eng; CAN
Character set
utf8; utf8 RI_458
Topic category
  • Oceans
  • Biota
  • Environment
Begin date
2023-08-11
End date
2023-10-16
N
S
E
W
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Reference system identifier
https://epsg.io / EPSG:4326 / 8.2.6
Distribution format
  • ESRI REST ( 2.9.8 )

  • CSV ( 1 )

  • FGDB/GDB ( 2.9.8 )

  • HTML ( N/A )

  • other ( FASTA File )

RI_412
  Government of Canada; Fisheries and Oceans Canada - Data Acquisition Assembly and Distribution / Acquisition, assemblage et distribution de donnée ( )
Bedford Institute of Oceanography, 1 Challenger Drive, PO Box 1006 , Dartmouth , Nova Scotia , B2Y 4A2 , Canada
OnLine resource
Raw DNA sequences ( HTTPS )

Supporting Document;HTML;eng

OnLine resource
12S-ASV DNA sequences ( HTTPS )

Dataset;ZIP;eng

OnLine resource
COI-ASV DNA sequences ( HTTPS )

Dataset;ZIP;eng

OnLine resource
12S taxonomy ( HTTPS )

Dataset;CSV;eng,fra

OnLine resource
DOTCOI taxonomy ( HTTPS )

Dataset;CSV;eng,fra

OnLine resource
Samples from DOT sampler and SmithRoot ( HTTPS )

Dataset;FGDB/GDB;eng,fra

OnLine resource
Samples from DOT sampler and SmithRoot ( HTTPS )

Dataset;CSV;eng,fra

OnLine resource
Data dictionary ( HTTPS )

Supporting Document;CSV;eng,fra

OnLine resource
Evaluating an Autonomous eDNA Sampler for Marine Environmental Monitoring: Short- and Long-Term Applications ( ESRI REST: Map Service )

Web Service;ESRI REST;eng

OnLine resource
Evaluating an Autonomous eDNA Sampler for Marine Environmental Monitoring: Short- and Long-Term Applications ( ESRI REST: Map Service )

Web Service;ESRI REST;fra

File identifier
436d5c50-8da6-483f-9705-91ecd6dc5c56 XML
Metadata language
eng; CAN
Character set
utf8; utf8 RI_458
Hierarchy level
dataset; jeuDonnées RI_622
Date stamp
2024-12-24T14:02:33.917Z
Metadata standard name
North American Profile of ISO 19115:2003 - Geographic information - Metadata
Metadata standard version
CAN/CGSB-171.100-2009
RI_414
  Government of Canada; Fisheries and Oceans Canada; Coastal Ecosystem Science Division (CESD) - CESD Data Manager / DSEC Gestionnaire de données ( Data Manager )
125 Marine Science Drive , St. Andrews , New Brunswick , E5B 0E4 , Canada
 
 

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Evaluating an Autonomous eDNA Sampler for Marine Environmental Monitoring: Short- and Long-Term Applications

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