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Atlantic bluefin tuna stomach contents 2018-2023, southern Gulf of St. Lawrence

PURPOSE:

This product serves a public facing webpage for the Canadian public to download Atlantic Bluefin Tuna stomach content data.

DESCRIPTION:

Metadata and stomach content from fish caught in the commercial fishery.

SAMPLING METHODS:

Stomachs were collected from Atlantic Bluefin Tuna (ABFT) caught from mid-August to late September over six years (2018–2023). Most samples originated from ABFT caught around the eastern end of Prince-Edward Island, which reflects the dominant ABFT fishing area, while a few samples were obtained from the Miscou/Baie-des-Chaleurs area in 2018 and 2019.

Fish were measured to the nearest curved fork length (cm) and weighed to the nearest round weight (kg). Stomachs were obtained directly from harvesters or through a fish buyer and were stored at −20 ◦C before being processed in the laboratory. Stomachs identification numbers were cross-referenced with ABFT tag numbers recorded by fish provider in order to obtain logbook and port data (catch location, time, weight length, sex, gear, etc.) for each sample. Stomachs were thawed in the laboratory and the content was sorted and identified to the lowest possible taxonomic level. For each stomach, prey were weighed collectively as a taxonomic group and individually to the nearest 0.1 g. Dead bait used to capture ABFT, identified by cut marks, were recorded and weighed but excluded from the analysis. Live bait items cannot be identified from stomach content analyses. Only a few otoliths were found in 2018 and their degraded quality precluded performing ageing or species identification. Rare and small prey items such as algae and rocks were classified in the category “other”. Fish remains that could not be identified were classified in the category “Unidentified teleostei remains”.

For 2019 to 2023, when stomach content items could not be visually identified and when tissue was available, tissue samples were collected and stored at −20 °C for DNA barcoding analysis. DNA extraction, mitochondrial cytochrome oxidase subunit 1 amplification, Sanger sequencing and species assignation were performed at the Plateforme d’Analyses Génomiques and Plateforme Bio-informatique of the Institut de Biologie Intégrative et des Systèmes (PAG-IBIS, Université Laval, Quebec city, QC, Canada, http://www.ibis.ulaval.ca/en/services-2/genomic-analysis-platform/ ). DNA was extracted from 20 mg of muscle tissue using the Omega Bio-tek E-Z-96 Tissue DNA Kit (Omega Bio-tek, Norcross GA, USA) following manufacturer instructions. The mitochondrial cytochrome oxidase subunit 1 region was amplified and sequenced as described in Hashemzadeh Segherloo et al., 2021). Sanger forward and reverse reads were analyzed independently using the Basic Local Alignment Search Tool against non-redundant sequences to identify the top hit for each sequence. When samples could not be identified by a top hit sequence they were classified as “unidentifiable fish”. Prey items that were successfully identified using DNA barcoding were incorporated into the stomach content analysis database and used in all subsequent diet analyses (abundance, occurrence and weight). The weight of the items used in the database was the weight of the remains as they were, and not reconstructed weights calculated for a live animal of the species identified by the barcoding.

USE LIMITATION:

To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.

Simple

Date ( RI_366 )
2018
Date ( RI_367 )
2025-02-27
RI_409
  Government of Canada; Fisheries and Oceans Canada - François Turcotte ( Aquatic Science Biologist )
343 Université Ave , Moncton , New Brunswick , E1C 5K4 , Canada
Purpose
This product serves a public facing webpage for the Canadian public to download Atlantic Bluefin Tuna stomach content data.
Status
completed; complété RI_593
Maintenance and update frequency
notPlanned; nonPlanifié RI_542
NASA/Global Change Master Directory (GCMD) Science Keywords Version 6.0.0.0.0 NASA/Global Change Master Directory (GCMD) Science Keywords Version 6.0.0.0.0 ( RI_528 )
  • Species/Population Interactions
Government of Canada Core Subject Thesaurus Thésaurus des sujets de base du gouvernement du Canada ( RI_528 )
  • Marine biology
DFO Areas Zones du MPO ( RI_525 )
  • Gulf of St. Lawrence
Use limitation
Open Government Licence - Canada (http://open.canada.ca/en/open-government-licence-canada)
Access constraints
license; licence RI_606
Use constraints
license; licence RI_606
Use limitation
To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.
Use limitation
n/a
Classification
unclassified; nonClassifié RI_484
Spatial representation type
textTable; texteTable RI_637
Metadata language
eng; CAN
Character set
utf8; utf8 RI_458
Topic category
  • Oceans
Begin date
2018
End date
2023
Description
Southern Gulf of St. Lawrence
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S
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Reference system identifier
http://www.epsg-registry.org / EPSG:4269
Distribution format
  • CSV ( n/a )

RI_414
  Government of Canada; Fisheries and Oceans Canada - François Turcotte ( Aquatic Science Biologist )
343 Université Ave , Moncton , New Brunswick , E1C 5K4 , Canada
OnLine resource
Atlantic bluefin tuna stomach contents 2018-2023, southern Gulf of St. Lawrence ( HTTPS )

Dataset;CSV;eng,fra

OnLine resource
Data dictionary ( HTTPS )

Supporting Document;CSV;eng,fra

OnLine resource
Supplementary citation list ( HTTPS )

Supporting Document;PDF;eng,fra

File identifier
93b46916-f2e0-11ef-88e3-5d0a2a9fd0c5 XML
Metadata language
eng; CAN
Character set
utf8; utf8 RI_458
Hierarchy level
dataset; jeuDonnées RI_622
Date stamp
2025-02-27T19:43:17.68Z
Metadata standard name
North American Profile of ISO 19115:2003 - Geographic information - Metadata
Metadata standard version
CAN/CGSB-171.100-2009
RI_414
  Government of Canada; Fisheries and Oceans Canada - François Turcotte ( Aquatic Science Biologist )
343 Université Ave , Moncton , New Brunswick , E1C 5K4 , Canada
 
 

Overviews

overview
Atlantic Bluefin Tuna

Spatial extent

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W
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