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    PURPOSE: To gather localized high-quality data for mapping eelgrass distribution in bays and estuaries in the Gulf Region of Atlantic Canada. DESCRIPTION: Between 2018 and 2023, a total of 48 coastal sites in New Brunswick (NB), Prince Edward Island (PE), and Nova Scotia (NS) have been fully processed for eelgrass presence/absence and depth information. An additional 18 sites from the same region and time period (2018–2023) have data collected but not yet fully processed for depth and eelgrass classification. These sites will be incorporated into the dataset as processing is completed. PARAMETERS COLLECTED: Geographic coordinates, timestamp, submerged aquatic vegetation presence. NOTES ON QUALITY CONTROL: BioSonics Visual Aquatic was used to process raw dt4 files by delineating the bottom and submerged aquatic vegetation (SAV) heights. Initial delineation of the estuary bottom was performed using an automated algorithm within the software, followed by manual adjustments to refine the delineation as needed. An algorithm was then used to delineate vegetation, which was edited visually by referring to written ground-truthing notes and underwater photos taken with a GoPro underwater camera with GPS capacity. Expert advice within DFO was used to advise the analysts on best practices and subtleties in the echograms. All efforts were made to ensure vegetation mapped was eelgrass, but in some cases, such as where the acoustic response was not clear or ground-truthing notes were lacking, it is possible that other types of SAV were included. The processed data were exported from BioSonics Visual Aquatic aggregating sets of 10 pings that were in very close proximity. Grouped pings with a vegetation canopy height >= 0.1 m were assigned an eelgrass presence (i.e., "EG_Presence") value of "Y", while grouped pings with a height < 0.1 m were assigned a presence value of "N". SAMPLING METHODS: Acoustic data were collected during the summer or early fall season (varies depending on the site) by the Southern Gulf of St. Lawrence Coalition on Sustainability (Coalition-SGSL) in partnership with Fisheries and Oceans Canada (DFO) Gulf Region. At some sites, the Province of New Brunswick's Department of Agriculture, Aquaculture and Fisheries (NBDAAF) also collected data using their boat. BioSonics MX Aquatic Habitat Echosounder units with a single beam (8.7°) 204.8kHz transducer (mounting height varied depending on the boat used) was used for data collection by all parties. Positioning was achieved using the BioSonics internal GPS through 2020, then subsequently an external GPS unit (Hemisphere S631 RTK GPS) was used to improve positioning from 1-2m accuracy to ~20cm when differential was obtained. BioSonics Visual Acquisition software was used to collect the data. USE LIMITATION: This product is provided as-is and has not been accuracy-assessed against other data. Since there were no transect-independent ground-truthing points surveyed, the accuracy of any interpolated surfaces created from this data cannot be known. Not for use without inclusion of full metadata. The data products are supplied "as they stand" and DFO does not guarantee the integrity, the completeness, or the accuracy. There were issues with the internal GPS of the BioSonics unit, and their impact on positional accuracy has yet to be determined. Beginning in 2021, an external, higher precision GPS unit was used to increase accuracy. Use of various boats and surveyors, as well as analysts, can introduce some inconsistencies in the data collection and analysis between sites and years. Site-specific characteristics such as mixed submerged aquatic vegetation can complicate mapping efforts. Shallow areas can also be challenging to delineate accurately since the bottom and/or the vegetation can extend higher than the mounted transducer. In these cases, a best estimate was used by the analyst. Weather conditions such as wind can affect the accuracy of the results, as the transponder may pitch and roll with the boat, while increased sediment in the water can interfere with the signal. Single-beam acoustic data has a very small focus footprint, which varies with depth, so it should not be considered a comprehensive bottom mapping tool. However, it does provide valuable point data that can indicate presence of vegetation, canopy height, relative depth, and ground-truthing for other mapping techniques (e.g. aerial or satellite imagery). For example, at 1 m depth, the 8.6 degree single-beam used for this work has a footprint of approximately 0.0177 square metres, and at 2 m depth that footprint becomes 0.0711 square metres.