Species characterization by environmental DNA (eDNA) is a method that allows the use of DNA released into the environment by organisms from various sources (secretions, faeces, gametes, tissues, etc.). It is a complementary tool to standard sampling methods for the identification of biodiversity. This project provides a list of fish and marine mammal species whose DNA has been detected in water samples collected between 2019 and 2021 using the mitochondrial marker MiFish (12S). The surveys were carried out in the summer of 2019 (July 14-18) and (July 30 - August 5), in the fall of 2020 (October 27-28) and in the summer-fall of 2021 (May 31 - June 3 ) and (August 24-25) between Forestville and Godbout (Haute-Côte-Nord). Sampling was carried out between 1-50 meters depth in 91 stations, with 1 to 3 replicates per station. Two liters of water were filtered through a 1.2 µm fiberglass filter. DNA extractions were performed with the DNeasy Blood and Tissues or PowerWater extraction kit (Qiagen). Negative field, extraction and PCR controls were added at the different stages of the protocol. The libraries were prepared either by Génome Québec (2019, 2020) or by the Genomics Laboratory of the Maurice-Lamontagne Institute (2021), then sequenced on a NovaSeq 4000 PE250 system by Génome Québec. The bioinformatics analysis of the sequences obtained was carried out using an analysis pipeline developed in the genomics laboratory. A first step made it possible to obtain a table of molecular operational taxonomic units (MOTU) using the cutadapt software for the removal of the adapters and the R package DADA2 for the filtration, the fusion, removal of chimeras and compilation of data. The MOTUs table was then corrected using the R package metabaR to eliminate the tag-jumping and take contaminants into consideration. Samples showing a strong presence of contaminating MOTUs were removed from the dataset. The MOTUs were also filtered to remove all remaining adapter sequences and also retain only those of the expected size (around 170 bp). Finally, taxonomic assignments were made on the MOTUs using the BLAST+ program and the NCBI-nt database. Taxonomic levels (species, genus or family) were assigned using a best match method (Top hit), with a threshold of 95%. Only assignments at the level of fish and marine mammals were considered, and the taxa detected were compared to a list of regional species, and corrected if necessary. The species detections of the different replicas have been combined. The file provided includes generic activity information, including site, station name, date, marker type, assignment types used for taxa identification, and a list of taxa or species. The list of taxa has been verified by a biodiversity expert from the Maurice-Lamontagne Institute. This project was funded by Fisheries and Oceans Canada's Coastal Environmental Baseline Data Program under the Oceans Protection Plan. This initiative aims to acquire baseline environmental data that contributes to the characterization of significant coastal areas and supports evidence-based assessments and management decisions to preserve marine ecosystems. Data were also published on SLGO platform : https://doi.org/10.26071/ogsl-2239bca5-c24a

A goal of the Government of Canada’s Coastal Environmental Baseline Program (CEBP) is to amalgamate historic environmental data from high vessel traffic areas. An extensive DFO biological sampling program was conducted from 1980 to 1981 on Sturgeon and Roberts banks located on the outer Fraser River estuary, BC, Canada. This report collates and simplifies three data sets: water quality and nearshore fish catch previously published as DFO Data Report 340 (Conlin et al.1982), and un- published weight-length (W-L) data for two locations on Sturgeon Bank (Iona and Steveston) and near Westshore Terminals (Coal Port) on Roberts Bank. W-L data were reconstructed from archived computer printouts using Optical Character Recognition methods. Analyses of water quality data indicate that the two banks provided different fish habitats with Sturgeon Bank having a greater freshwater influence. Although Iona area water quality was exposed to sewage outfall from a nearby sewage treatment plant, it appears that fish communities were not different from the other Sturgeon Bank area (Steveston). The fish communities were found to be different between the two banks with Roberts Bank having greater overall abundance and diversity. Interestingly, of the seven fish species used for condition factor analyses, five were found to have lower Relative Condition Factors in the Roberts Bank sampling area.

From August 6th to September 9th, 2014, Fisheries and Oceans Canada conducted a baseline survey of marine fishes and their habitats on the Canadian Beaufort Shelf and slope. Sampling was conducted from the F/V Frosti at 56 stations along thirteen transects, with an additional three stations not located on transects. Standardized sampling was conducted on the transects at pre-determined depth stations (20-40, 75, 200, 350, 500, 750, and 1000 m) using a variety of sampling equipment including benthic fishing trawls, plankton nets, sediment cores, and CTD and water sample profiles. Presented here is the information on the sampling locations, and the sampling gear deployed at each station.

This dataset contains observations of species occurrences from seafloor imagery collected by the autonomous underwater vehicle (AUV) during the 2012 Expedition to Cobb Seamount. The National Oceanographic and Atmospheric Administration-operated SeaBED-class AUV which collected photographic images from 4 transects ranging from 436 m to 1154 m in depth.

PURPOSE: To track juvenile Atlantic salmon densities. DESCRIPTION: Indices of freshwater production are derived annually from electrofishing surveys in the rivers of Nova Scotia flowing into the Gulf of St. Lawrence. Fixed site sampling for juvenile salmon has been conducted most consistently since the mid-1980s for these rivers. Juvenile salmon abundances at sites, in terms of number of fish per habitat area sampled by age or size group (densities), are obtained using successive removal sampling or catch per unit effort sampling calibrated to densities. Sampling intensities vary among years and among rivers. PARAMETERS COLLECTED: distribution (ecological); species counts (ecological); point (spatial) USE LIMITATION: To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.

Summary The Quebec region of the Department of Fisheries and Oceans (DFO) is responsible for the assessment of several fish and invertebrate stocks exploited in the Estuary and the northern Gulf of St. Lawrence. The commercial catches sampling program is one of the sources of information used to complete these assessments. The data collected by this program, at wharf or at sea, offers among other things the advantage of a relatively large spatio-temporal coverage and provides some of the necessary knowledge to assess the demography and the structure of the exploited populations. This program is implemented by specialized DFO staff whose main mandate is to collect biological data on groundfish, pelagic fish and marine invertebrate species that are commercially exploited in the various marine communities. Data This dataset on the Atlantic cod (Gadus morhua) includes the metadata, sample weight, fish length, the sex and the number of specimens measured. This dataset covers the period of 1983 to present. In order to protect the confidentiality of the sources, some informations (such as those concerning the vessel) have been excluded and others (such as the date of capture) have been simplified. Entries where there was only one vessel in a fishing area for a given year were also excluded. Further information including the fishing areas coordinates can be found by clicking on the «Atlantic and Arctic commercial fisheries» and «Fishing areas» links below.

This resource documents a dataset of epifauna occurrences collected in 2021 during The Knowledge and Ecosystem-Based Approach in Baffin Bay (KEBABB) program developed by the Department of Fisheries and Oceans Canada (DFO) in collaboration with university partners. The overall objective of KEBABB is to characterize the variability and trends in physical, chemical, and biological oceanographic conditions and food webs supporting fisheries in the connected ecosystems of western Baffin Bay and Lancaster Sound. In 2021, DFO expanded the KEBABB program to Barrow Strait (KEBABS-Knowledge and Ecosystem-Based Approach in Barrow Strait), a key productive area of the Tallurutiup Imanga National Marine Conservation Area. The study took place in the Eastern Canadian Arctic (mainly in Baffin Bay, Davis Strait and Barrow Strait). Sampling is done along transects at fixed stations in the study area. Catches are collected with a 1.5 m Agassiz trawl (5 mm mesh net) for 3 minutes bottom-contact time at a target speed of 1.5 knots and with a 3 m benthic beam trawl (6.4 mm mesh net) for 15 minutes bottom-contact time at a target speed of 3 knots. A total of 16 stations were sampled for epifauna in 2021 between 85-850 m depth. Epibenthic invertebrates are identified to the lowest possible taxonomic level and photographed. All unknown specimens are frozen. In the lab, the identifications are validated or refined with the photos and the frozen specimens. The data are presented in Darwin Core and are separated in two files: The “Activité_épifaune_KEBABB_epifauna_event_en” file which contains information about missions, stations and deployments, which are presented under a hierarchical activity structure. The “Occurrence_épifaune_KEBABB_epifauna_en” file that contains the taxonomic occurrences. Further details on sampling can be found in the following report: Pućko, M., Charette, J., Tremblay P., Brulotte S., St-Denis B., Ciastek S., Hedges, K., Kuzyk, Z., Roy V., and Michel, C. 2022. An ecosystem-based approach in the eastern Arctic: KEBABB/S (Knowledge and Ecosystem-Based Approach in Baffin Bay/Barrow Strait) 2021 expedition report. Can. Manuscr. Rep. Fish. Aquat. Sci. 3250: viii + 58 p. https://publications.gc.ca/collections/collection_2022/mpo-dfo/Fs97-4-3250-eng.pdf USE LIMITATION: To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.

Cold-water corals are conspicuous in the waters off Eastern Canada. Despite that, there are few DNA sequence records from specimens collected in the region available in GenBank, and not all species recorded in the region have sequence data regardless of geographic origin. This can limit the use of eDNA techniques to detect and identify corals. Our objective was to sequence and publish sequences for two octocoral DNA barcoding markers: CO1 and MutS. We sequenced and deposited 36 sequences to GenBank from 19 specimens representing three sea pen taxa (Octocorallia: Pennatuloidea): Distichoptilum gracile, Pennatula aculeata, and Protoptilum carpenteri. Identification of all specimens was confirmed by B. M. Neves before submission. Specimens and DNA tissues were donated to the Canadian Museum of Nature, where they are currently stored. This publication is part 1 of a series of GenBank submissions by our lab. Specimens were collected from across the Northwest Atlantic and originate from depths ranging between 200-1924 meters. Specimens were collected as part of research vessel multispecies trawl surveys or remotely operated vehicle (ROV ROPOS) surveys. DNA was isolated and purified using the QIAgen DNeasy Blood and Tissue kit, with an initial overnight incubation with Proteinase K. Two commonly used octocoral barcoding regions were amplified using previously described primers: 1) COII8068F (McFadden et al., 2004) and COIOCTR (France and Hoover, 2002) for the CO1 gene, and 2) ND42599F (France and Hoover, 2002) and mut3458R (Sánchez et al., 2003) for the MutS gene. Amplifications were conducted using 12.5 µl of Green DreamTaq Master Mix (Thermo Fisher Scientific), 1 µl of template DNA, 0.5 µl of each 10 µM forward and reverse primers, 0.5 µl of 10 µM reverse primer, and 10.5 µl of water. Thermocycling was run as follows: 3 min of initial denaturation at 95 °C, followed by 40 cycles at 95 °C for 30 s, 30 s at annealing temperature of 48 °C, then 65 s at an extension temperature of 72 °C, and a final elongation at 72 °C for 4 min. PCR products were cleaned using Agencourt AMPure XP Beads (Beckman Coulter) and sent to The Center for Advanced Genomics, Toronto, Canada for Sanger sequencing. Sequences were visualized and aligned using Geneious Prime 2022.0.2. Obtained sequences have been deposited in GenBank under accession numbers OQ569768- OQ569784 and OQ420359- OQ420377. This work was funded by Fisheries and Oceans Canada under an Enhanced Regional Capacity grant (2020-2021) and the Marine Conservation Targets (MCT) program (2021-2024), Newfoundland and Labrador Region.

Summary The Quebec region of the Department of Fisheries and Oceans (DFO) is responsible for the assessment of several fish and invertebrate stocks exploited in the Estuary and the northern Gulf of St. Lawrence. The commercial catches sampling program is one of the sources of information used to complete these assessments. The data collected by this program, at wharf or at sea, offers among other things the advantage of a relatively large spatio-temporal coverage and provides some of the necessary knowledge to assess the demography and the structure of the exploited populations. This program is implemented by specialized DFO staff whose main mandate is to collect biological data on groundfish, pelagic fish and marine invertebrate species that are commercially exploited in the various marine communities. Data This dataset on Greenland halibut (Reinhardtius hippoglossoides) includes the metadata, sample weight, fish length, the sex and the number of specimens measured. This dataset covers the period of 1987 to present. In order to protect the confidentiality of the sources, some informations (such as those concerning the vessel) have been excluded and others (such as the date of capture) have been simplified. Entries where there was only one vessel in a fishing area for a given year were also excluded. Further information including the fishing areas coordinates can be found by clicking on the «Atlantic and Arctic commercial fisheries» and «Fishing areas» links below.

Fisheries and Oceans Canada (DFO) has been conducting surface water trawl surveys since 1992 in the coastal waters of British Columbia, Washington, Oregon and Alaska and in the high seas of the Gulf of Alaska. These surveys initially focused on determining the migratory patterns (1992-2002) and on the growth and physiology (2003-2016) of juvenile Pacific Salmon. Data collected in shelf and slope waters at depths less than 400 meters off Washington and Oregon State are part of this larger survey series, but are limited to 1999-2001. These surveys focused on determining the migratory patterns of juvenile Pacific Salmon and had funding support from the Bonneville Power Administration as part of the 1995-2011 Canada-USA Salmon Shelf Survival Study. The intent of that study was to monitor and evaluate the effects of ocean conditions on the distribution, migration, growth, and survival of Pacific salmon during their first ocean year, and estimate the subsequent impacts on abundance of Chinook salmon adults returning to the Columbia River system.