Species characterization by environmental DNA (eDNA) is a method that allows the use of DNA released into the environment by organisms from various sources (secretions, faeces, gametes, tissues, etc.). It is a complementary tool to standard sampling methods for the identification of biodiversity. This project provides a list of fish and marine mammal species whose DNA has been detected in water samples collected between 2019 and 2021 using the mitochondrial marker MiFish (12S). The surveys were carried out in the summer of 2019 (July 14-18) and (July 30 - August 5), in the fall of 2020 (October 27-28) and in the summer-fall of 2021 (May 31 - June 3 ) and (August 24-25) between Forestville and Godbout (Haute-Côte-Nord). Sampling was carried out between 1-50 meters depth in 91 stations, with 1 to 3 replicates per station. Two liters of water were filtered through a 1.2 µm fiberglass filter. DNA extractions were performed with the DNeasy Blood and Tissues or PowerWater extraction kit (Qiagen). Negative field, extraction and PCR controls were added at the different stages of the protocol. The libraries were prepared either by Génome Québec (2019, 2020) or by the Genomics Laboratory of the Maurice-Lamontagne Institute (2021), then sequenced on a NovaSeq 4000 PE250 system by Génome Québec. The bioinformatics analysis of the sequences obtained was carried out using an analysis pipeline developed in the genomics laboratory. A first step made it possible to obtain a table of molecular operational taxonomic units (MOTU) using the cutadapt software for the removal of the adapters and the R package DADA2 for the filtration, the fusion, removal of chimeras and compilation of data. The MOTUs table was then corrected using the R package metabaR to eliminate the tag-jumping and take contaminants into consideration. Samples showing a strong presence of contaminating MOTUs were removed from the dataset. The MOTUs were also filtered to remove all remaining adapter sequences and also retain only those of the expected size (around 170 bp). Finally, taxonomic assignments were made on the MOTUs using the BLAST+ program and the NCBI-nt database. Taxonomic levels (species, genus or family) were assigned using a best match method (Top hit), with a threshold of 95%. Only assignments at the level of fish and marine mammals were considered, and the taxa detected were compared to a list of regional species, and corrected if necessary. The species detections of the different replicas have been combined. The file provided includes generic activity information, including site, station name, date, marker type, assignment types used for taxa identification, and a list of taxa or species. The list of taxa has been verified by a biodiversity expert from the Maurice-Lamontagne Institute. This project was funded by Fisheries and Oceans Canada's Coastal Environmental Baseline Data Program under the Oceans Protection Plan. This initiative aims to acquire baseline environmental data that contributes to the characterization of significant coastal areas and supports evidence-based assessments and management decisions to preserve marine ecosystems. Data were also published on SLGO platform : https://doi.org/10.26071/ogsl-2239bca5-c24a

This resource documents a dataset of epifauna occurrences collected in 2021 during The Knowledge and Ecosystem-Based Approach in Baffin Bay (KEBABB) program developed by the Department of Fisheries and Oceans Canada (DFO) in collaboration with university partners. The overall objective of KEBABB is to characterize the variability and trends in physical, chemical, and biological oceanographic conditions and food webs supporting fisheries in the connected ecosystems of western Baffin Bay and Lancaster Sound. In 2021, DFO expanded the KEBABB program to Barrow Strait (KEBABS-Knowledge and Ecosystem-Based Approach in Barrow Strait), a key productive area of the Tallurutiup Imanga National Marine Conservation Area. The study took place in the Eastern Canadian Arctic (mainly in Baffin Bay, Davis Strait and Barrow Strait). Sampling is done along transects at fixed stations in the study area. Catches are collected with a 1.5 m Agassiz trawl (5 mm mesh net) for 3 minutes bottom-contact time at a target speed of 1.5 knots and with a 3 m benthic beam trawl (6.4 mm mesh net) for 15 minutes bottom-contact time at a target speed of 3 knots. A total of 16 stations were sampled for epifauna in 2021 between 85-850 m depth. Epibenthic invertebrates are identified to the lowest possible taxonomic level and photographed. All unknown specimens are frozen. In the lab, the identifications are validated or refined with the photos and the frozen specimens. The data are presented in Darwin Core and are separated in two files: The “Activité_épifaune_KEBABB_epifauna_event_en” file which contains information about missions, stations and deployments, which are presented under a hierarchical activity structure. The “Occurrence_épifaune_KEBABB_epifauna_en” file that contains the taxonomic occurrences. Further details on sampling can be found in the following report: Pućko, M., Charette, J., Tremblay P., Brulotte S., St-Denis B., Ciastek S., Hedges, K., Kuzyk, Z., Roy V., and Michel, C. 2022. An ecosystem-based approach in the eastern Arctic: KEBABB/S (Knowledge and Ecosystem-Based Approach in Baffin Bay/Barrow Strait) 2021 expedition report. Can. Manuscr. Rep. Fish. Aquat. Sci. 3250: viii + 58 p. https://publications.gc.ca/collections/collection_2022/mpo-dfo/Fs97-4-3250-eng.pdf USE LIMITATION: To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.

Fisheries and Oceans Canada (DFO) conducts an annual summer multidisciplinary scientific survey with a bottom trawl in the Estuary and the northern Gulf of St. Lawrence since 1984. Over the years this survey has been conducted on four vessels: the MV Lady Hammond (1984-1990), the CCGS Alfred Needler (1990-2005), the CCGS Teleost (2004-2022) and the CCGS Cabot (2022-current). It is important to note that the objectives, the methods used and the identification of the species during these surveys have improved over time in response to DFO requests and mandates. The data are therefore not directly comparable between these surveys. However, comparative analyses have been carried out between vessels, and conversion factors are available upon request for a number of species. The specificities of the missions onboard the CCGS Cabot are described below. Objectives: 1. Assess groundfish and northern shrimp population abundance and condition 2. Assess environmental conditions 3. Conduct a biodiversity inventory of benthic and demersal megafauna 4. Assess phytoplankton and mesozooplankton abundance 5. Monitor the pelagic ecosystem 6. Collect samples for various research projects Survey description The survey covers the Estuary and the northern Gulf of St. Lawrence, that is the divisions 4R, 4S and the northern part of division 4T of the Northwest Atlantic Fisheries Organization (NAFO). Since 2008, coverage of division 4T has been increased in the upstream part of the Lower Estuary. Approximately 200 sampling stations are randomly attributed in depth-based strata, ranging from around 40 m near the shores to close to 500 m in the deep channels. The fishing gear used on the CCGS Cabot is a four-sided modified Campelen 1800 shrimp trawl equipped with a Rockhopper footgear (“bicycle”). The trawl lengthening and codend are equipped with a 12.7-mm knotless nylon lining. Standard trawling tows last 15 minutes, starting from the time the trawl touches the sea floor. The aimed towing speed is 3 knots. Data For each fishing tow, the catch is sorted and weighed by taxa; individuals are then counted and biological data are collected on a subsample. For fish, crab and squid, size and weight are measured by individual and, for some species, sex, gonad maturity, and the weight of certain organs (stomach, liver, gonads) are also evaluated. The soft rays of the anal fin are counted for redfish, and the otoliths are sampled for several species such as Atlantic cod, Atlantic halibut, Greenland halibut and witch flounder. A roughly 2-kg shrimp sample is sorted and weighed by species (and by stage of maturity for northern shrimp). The shrimps are measured individually. The other invertebrates are counted (no individual measurements) and photographed. The biological data are divided into 4 files: a “Metadata” file containing set information, a “Catches” file containing catches per set for fish taxa, a “Carbio” file containing biological and morphometric measurements per individual and a “Shrimps” file containing information on shrimp catches. The latest published data is preliminary and will be updated at the end of January of the following year. It's important to note that this is raw data. Only sets considered successful are retained. In each set, all species are kept, with a few exceptions. For more information please contact the data management team (gddaiss-dmsaisb@dfo-mpo.gc.ca).

Situated in the Gwich’in settlement Area (GSA), the Rat River is inhabited by anadromous Dolly Varden (Salvelinus malma malma) that are harvested by both Gwich’in and Inuvialuit beneficiaries. The harvest of Dolly Varden from the Rat River occurs during the summer at feeding areas along the coast (by the Inuvialuit) and during upstream migration in the Mackenzie Delta (by both Gwich’in and Inuvialuit). Dolly Varden stocks are co-managed under an Integrated Fisheries Management Plan (IFMP) whose signatories include Fisheries and Oceans Canada (DFO), Gwich'in Renewable Resources Board, Fisheries Joint Management Committee, and Parks Canada Agency. The Rat River Working Group, the co-management body that makes recommendations for harvest levels for Dolly Varden stocks in the GSA, has supported research activities that facilitate implementation of the IFMP, including studies to monitor harvest levels and assess population status. Population studies (e.g., abundance estimates, biological and genetic sampling) and coastal harvest monitoring activities allow for a comprehensive assessment of this stock. The data are used to inform co-management partners on the status of Dolly Varden from the Rat River.

The Canadian Environmental Sustainability Indicators (CESI) program provides data and information to track Canada's performance on key environmental sustainability issues. Canada's conserved areas indicators report the amount and proportion of Canada's terrestrial (land and freshwater) and marine area that is recognized as conserved. Well-managed conserved areas are one way to protect wild species and their habitats for present and future generations. Habitat conservation is a measure of human response to the loss of biodiversity and natural habitat. As the area conserved in Canada increases, more lands and waters are withdrawn from direct human development stresses, thereby contributing to biodiversity conservation and improving the health of ecosystems. In turn, healthy ecosystems provide benefits such as clean water, mitigation of climate change, pollination and improved human health. Information is provided to Canadians in a number of formats including: static and interactive maps, charts and graphs, HTML and CSV data tables and downloadable reports. See the supplementary documentation for the data sources and details on how the data were collected and how the indicator was calculated. Canadian Environmental Sustainability Indicators: https://www.canada.ca/environmental-indicators

The objective of this project was to gather data to develop a model of the food web of the lower trophic levels of the nearshore area of the Beaufort Sea. Sampling took place from 2005 to 2008 using the CCGS Nahidik. The multidisciplinary character of the Nahidik program produced measurements of biology/ecology (primary production, phytoplankton, zooplankton, benthos, fish), chemical and physical oceanography, contaminants, geology and hydro acoustics. The data were collected in July and August of each year. The Nahidik program provided data to provide a baseline for future studies as well as an information source for environmental assessment. This record contains water chemistry data collected as part of this project including suspended nitrogen, dissolved nitrogen, suspended phosphorus, dissolved phosphorus, dissolved organic carbon, suspended carbon, chlorophyll a, and suspended silicon.

Each summer, environmental community groups collect important data to determine if groupings of fish, shrimp and crab – what is called a community- can be used as an indicator of the health status of bays and estuaries. Sampling was conducted from May through September for the first years then from June through August. In 2018 and 2019, the sampling was conducted just once in each estuary. Community group members and staff sample six stations once a month in their designated estuary. Fish, shrimps and crabs are collected with a beach seine net and later released live back to the water once identified and counted. From this, the community groups provide important information to Fisheries and Oceans Canada, including: - identification and numbers of fish, shrimp and crab species; - water conditions and samples; - information on aquatic plants; - sediment samples. With this information, Fisheries and Oceans Canada scientists working with government agencies and universities can conduct analyses to determine the suitability of indicators to assess the health of bays and estuaries. PARAMETERS COLLECTED: Parameters: abundance, species richness, species developmental stage (young-of-the-year or adult), water temperature, water salinity, water dissolved oxygen, dissolved inorganic nutrient (nitrate, nitrite, phosphate), sediment % organic content, sediment % humidity content and sediment mean grain size, % submerged aquatic vegetation cover NOTES ON QUALITY CONTROL: Data entry into Excel and first quality control verification is done by CAMP summer students. A second quality control verification is done by DFO staff. See publ # 2823 attached to this record. In 2018, the historical data was migrated into a relationship database. From this year on, annual data will be entered into the database using a custom application. The application front end has numerous QC elements built-in. SAMPLING METHODS: Please see the following URL for sampling details: http://www.dfo-mpo.gc.ca/Library/319437.pdf

This dataset contains annual mean stream water flow/discharge data derived from daily means for headwater streams draining forested hillslopes measured at stream catchments C31, C32, C33, C34, C35, C37, C38, C39, C42, C46, C47, C49, and C50 in the Turkey Lakes Watershed, approximately 60 km northwest of Sault Ste. Marie, Ontario, Canada. This data set is recorded as annual mean flow by calendar year (January-December in litres per second), annual mean flow by water year (October to September in litres per second), and annual number of zero flow days by water year. Daily mean flows that were used to derive this data set were recorded from 1981-2012 by the Great Lakes Forestry Centre, and are reported for 1981-2011 due to some inaccuracies throughout the 2012 data. Hydrological gauging stations employ "flow-control" 120 degree V-notch weirs (catchments 31, 33, 34, 37, 39, 42, 46, and 47), 90 degree V-notch weirs (catchments 32, 35, and 49), and 60 degrees V-notch weirs (catchment 38) to facilitate monitoring of stream discharge. Water "stage" or depth within the structure (e.g. in the pool behind the weir notch) is automatically and continuously recorded by chart recorders (Leupold & Stevens A-71 SE Water Level Recorder) from 1981-2003. Capacitance rods (Trutrack WT-HR 1000) installed in stilling wells replaced the chart recorders over the period of 2002 to 2003 and were used until 2012. The capacitance rods logged data at 1 hour intervals for the majority of their use and then averaged to a daily rate. Manual stage measurements have been taken intermittently when synoptic water chemistry samples were taken throughout the years. Stage data are then converted to a continuous record of flow using the relationship between stage and discharge measurements.

From August 6th to September 9th, 2014, Fisheries and Oceans Canada conducted a baseline survey of marine fishes and their habitats on the Canadian Beaufort Shelf and slope. Sampling was conducted from the F/V Frosti at 56 stations along thirteen transects, with an additional three stations not located on transects. Standardized sampling was conducted on the transects at pre-determined depth stations (20-40, 75, 200, 350, 500, 750, and 1000 m) using a variety of sampling equipment including benthic fishing trawls, plankton nets, sediment cores, and CTD and water sample profiles. Presented here is the information on the sampling locations, and the sampling gear deployed at each station.

PURPOSE: The primary objective of this survey is to obtain an index of the abundance of American lobster (Homarus Americanus) in the Northumberland Strait. This research survey also provides fisheries-independent information for all of the species captured by the trawl. DESCRIPTION: Catch weight, length frequencies, and biological information for crustaceans and fish caught during the annual July-August research vessel trawl survey in Northumberland Strait (NAFO Division 4T). Abundance indices and spatial distribution patterns of commercial and non-commercial crustaceans and other groundfish. PARAMETERS COLLECTED: The full catch is sorted to species, or lowest taxonomic group possible, and weighed. For lobster, carapace length, sex and carapace condition (i.e. stage of moult) are recorded. For female lobster, the presence or absence of eggs is noted and, starting in 2010, the stage of development of the eggs (i.e. new or old) when present was also recorded. Similarly, for crabs, the carapace width, sex and carapace condition (i.e. stage of moult) is recorded as is the presence or absence of eggs (for females). Fish sampling varied over the years but, starting in 2005, length has been recorded for all fish captured. For large catches, the complete catch is sorted and weighed, but sub-sampling, by species, is used for the other measurements. NOTES ON QUALITY CONTROL: Data are checked for irregularities. SAMPLING METHODS: The Northumberland Strait multi-species survey began in 2001 as a random stratified survey and now employs a random selection of sampling sites within the study area. Sampling is completed annually over a 4-week period in July and August. 110 stations are sampled in Northumberland Strait in water depths exceeding 4 m at Lowest Normal Tide. The survey has employed a 286 rockhopper trawl in all years from 2001 to 2018, except in 2010 and 2011 when a Nephrops trawl was used. In 2019, a newly designed trawl (termed the “Northumberland trawl”) was used in addition to the rockhopper trawl. The Northumberland trawl is similar to the rockhopper trawl but with a smaller footgear and smaller mesh sizes. At each station, the trawl is towed for a set period of time (recently 15 minutes with the rockhopper trawl and 10 minutes with the Northumberland trawl). USE LIMITATION: To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.