PURPOSE: Establishing efficient, non-destructive sampling methods for clam population assessments. DESCRIPTION: In the Gulf of St. Lawrence (GSL) Management Region, clam assessments are uncommon due to limited resources and the labour-intensive nature of sampling clam beds. Furthermore, clam assessments typically rely on destructive sampling that disturbs sediment and removes animals from their habitat. Establishing efficient, non-destructive sampling methods for clam population assessments can reduce the impact of scientific sampling on these habitats and provide for more efficient monitoring. In this study, we tested the idea that visually observing siphon holes on the sediment surface could predict the presence, number, and size of soft-shell clams across different sites in the southern GSL. Siphon holes reasonably predicted the presence, number, and size/biomass of soft-shell clams in most, but not all, sites. Thus, in many habitats in the GSL, siphon holes can be used for population assessments, providing a powerful tool to enhance Science advice to fisheries managers. Data was collected at the following sites: * Maisonnette, Parc Maisonnette, Maisonnette, New Brunswick, Canada * Kouchibouguac, Loggiecroft wharf, Kouchibouguac National Park, New Brunswick, Canada * Shemogue, Amos Point Road, Little Shemogue, New Brunswick, Canada * Powell's Cove, Powell's Point Provincial Park, Little Harbour, Nova Scotia, Canada PARAMETERS COLLECTED: - Clam abundance - Clam biomass (total sample) - Clam size (length, weight) - Siphon hole abundance - Siphon hole size - Siphon hole characterization (i.e., identification of actual clam based on shape) - Seawater temperature - Sediment grain size - Sediment organic content (%) - Sediment relative moisture content (%) NOTES ON QUALITY CONTROL: Original data entry by Jillian Hunt and/or Isabelle Brennan. Data checked and validated prior to analysis by Jeff Clements. Data further checked and validated prior to publication by Amélie Robichaud. PHYSICAL SAMPLE DETAILS: No physical samples retained. - Clam samples returned back to original habitat after measuring and weighing in the field. - Sediment core samples stored in walk-in freezer and discarded after processing and analysis. SAMPLING METHODS: i. Identifying, counting, weighing, and measuring (with calipers) clams ii. Identifying, counting, and measuring (with calipers) clam siphon holes iii. Seawater temperature monitoring via data loggers iv. Sediment grain size, organic content, and moisture content analysis USE LIMITATION: To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.

The Aquatic Invasive Species Surveillance Database is a compilation of fish community and habitat data from DFO’s Aquatic Invasive Species and Invasive Carp Program early detection surveillance efforts in Canadian waters of the Great Lakes basin. Data includes: sampling site location, date, fish species and counts, and associated habitat information. Annual project-specific details including purpose/objectives and study methodology are often reported in the DFO Canadian manuscript report of fisheries and aquatic sciences series.

Cold-water corals are conspicuous in the waters off Eastern Canada. Despite that, there are few DNA sequence records from specimens collected in the region available in GenBank, and not all species recorded in the region have sequence data regardless of geographic origin. This can limit the use of eDNA techniques to detect and identify corals. Our objective was to sequence and publish sequences for two octocoral DNA barcoding markers: CO1 and MutS. We sequenced and deposited 36 sequences to GenBank from 19 specimens representing three sea pen taxa (Octocorallia: Pennatuloidea): Distichoptilum gracile, Pennatula aculeata, and Protoptilum carpenteri. Identification of all specimens was confirmed by B. M. Neves before submission. Specimens and DNA tissues were donated to the Canadian Museum of Nature, where they are currently stored. This publication is part 1 of a series of GenBank submissions by our lab. Specimens were collected from across the Northwest Atlantic and originate from depths ranging between 200-1924 meters. Specimens were collected as part of research vessel multispecies trawl surveys or remotely operated vehicle (ROV ROPOS) surveys. DNA was isolated and purified using the QIAgen DNeasy Blood and Tissue kit, with an initial overnight incubation with Proteinase K. Two commonly used octocoral barcoding regions were amplified using previously described primers: 1) COII8068F (McFadden et al., 2004) and COIOCTR (France and Hoover, 2002) for the CO1 gene, and 2) ND42599F (France and Hoover, 2002) and mut3458R (Sánchez et al., 2003) for the MutS gene. Amplifications were conducted using 12.5 µl of Green DreamTaq Master Mix (Thermo Fisher Scientific), 1 µl of template DNA, 0.5 µl of each 10 µM forward and reverse primers, 0.5 µl of 10 µM reverse primer, and 10.5 µl of water. Thermocycling was run as follows: 3 min of initial denaturation at 95 °C, followed by 40 cycles at 95 °C for 30 s, 30 s at annealing temperature of 48 °C, then 65 s at an extension temperature of 72 °C, and a final elongation at 72 °C for 4 min. PCR products were cleaned using Agencourt AMPure XP Beads (Beckman Coulter) and sent to The Center for Advanced Genomics, Toronto, Canada for Sanger sequencing. Sequences were visualized and aligned using Geneious Prime 2022.0.2. Obtained sequences have been deposited in GenBank under accession numbers OQ569768- OQ569784 and OQ420359- OQ420377. This work was funded by Fisheries and Oceans Canada under an Enhanced Regional Capacity grant (2020-2021) and the Marine Conservation Targets (MCT) program (2021-2024), Newfoundland and Labrador Region.

Commercial and exploratory fisheries for Arctic Char, Salvelinus alpinus, provide significant economic opportunities for Nunavummiut in several Nunavut communities. Having an accurate understanding of the weight of the Arctic Char that are harvested is important for tracking harvest and for understanding how biological parameters may be changing over time as a result of exploitation and/or climactic and environmental changes. Unfortunately, most fish enter the processing plants as dressed (gills and viscera removed) and therefore conversion factors have to be applied to reconcile whole (round) weight from dressed weight. Here, we provide an updated conversion factor based on linear regression for Arctic Char from the Halokvik River (locally known as 30 Mile) near the community of Cambridge Bay. This conversion factor can be used moving forward as the Halokvik River continues to be commercially harvested.

These data sets provide information pertaining to abundant taxa including bottom-dwelling shrimp and fish along trawling and trapping transects in Simoom Sound at November, 2000, and February, 2001. Data sets were compiled and formatted by Meagan Mak. Abstract from report: This study is a component of a larger project designed to compare the effects of shrimp trawling and trapping gear on shrimp, fish and the benthic habitat of Simoom Sound located in Broughton Archipelago, British Columbia, Canada. Otter-trawling, beam-trawling, and trapping took place in three distinct experiment blocks of the central seabed of the Sound. In turn, each block consisted of replicate transects, where a towed submersible video-camera surveyed shrimp and fish before and after trawling. Video surveys were deployed only before trapping. From the video surveys, we determined the abundance of common shrimp taxa and fish.

These data sets provide information pertaining to shrimp and bycatch estimates associated with otter-trawling and trapping (November, 2000) and beam-trawling (February, 2001) in Simoom Sound. Data sets were compiled and formatted by Meagan Mak. Abstract from report: As part of a project investigating possible modification of marine ecosystems by shrimp trawling and trapping, we obtained information on catches offish, shrimp, prawns , and bycatch organisms as well as weight, sex ratios , egg location and colIateral damage to several species of pandalids and eualids. Focusing on the humpback shrimp (Pandalus hypsinotus), we assessed damage to the rostrum, carapace, abdomen, and tail fan on specimens caught by beam trawling, otter trawling, and trapping. Data are given from a preliminary study conducted in Northumberland Channel in June 2000 and more comprehensive sampling from Simoom Sound in November 2000 and February 2001.

The Great Lakes Fish Biodiversity Science Database is a compilation of fish community and habitat data from DFO Science surveys, primarily related to freshwater fishes of conservation concern in the Great Lakes basin. Data include: sampling site location, date, fish species and counts, and associated habitat information. Project-specific details including purpose/objectives and study methodology are often reported in the DFO Canadian data report of fisheries and aquatic sciences series.

This dataset contains observations of species occurrences from seafloor imagery collected by the autonomous underwater vehicle (AUV) during the 2012 Expedition to Cobb Seamount. The National Oceanographic and Atmospheric Administration-operated SeaBED-class AUV which collected photographic images from 4 transects ranging from 436 m to 1154 m in depth.

These data sets provide information pertaining to shrimp and bycatch estimates associated with beam-trawling and trapping (2001-2002) in Clio Channel. Data sets were compiled and formatted by Meagan Mak. Abstract from report: As part of a project investigating possible modification of marine ecosystems by shrimp trawling and trapping, we enumerated beam trawl and prawn trap catches at two locations in Clio Channel, south -central coast of British Columbia. Beam trawl surveys were conducted in Bones Bay and Turnour Bay during October 2001 and January 2002, respectively, and a prawn trap survey was conducted in Turnour Bay during March 2002. Catch data from the two gear types are presented.

Fisheries and Oceans Canada (DFO) conducts an annual summer multidisciplinary scientific survey with a bottom trawl in the Estuary and the northern Gulf of St. Lawrence since 1984. Over the years, this survey has been conducted on four vessels: the MV Lady Hammond (1984-1990), the CCGS Alfred Needler (1990-2005), the CCGS Teleost (2004-2021) and the CCGS Cabot (2022-current). It is important to note that the objectives, the methods used and the identification of the species during these surveys have improved over time in response to DFO requests and mandates. The data are therefore not directly comparable between these surveys. The specificities of the missions onboard the MV Lady Hammond are described below. Objectives: 1. Assess groundfish populations abundance and condition 2. Assess environmental conditions 3. Conduct a biodiversity inventory of benthic and demersal megafauna 4. Monitor the pelagic ecosystem 5. Collect samples for various research projects Survey description The survey covers the northern Gulf of St. Lawrence, that is the divisions 4R, 4S and the northern part of division 4T of the Northwest Atlantic Fisheries Organization (NAFO). A stratified random sampling strategy is used for this survey and the fishing gear used on the MV Lady Hammond is a bottom trawl Western IIA. Standard trawling tows last 30 minutes, starting from the time the trawl touches the sea floor. Towing speed is 3.5 knots. Data For each fishing tow, the catch is sorted and weighed by taxa; individuals are counted and biological data are collected on a sub-sample. For fish, crab and squid, size and weight are measured by individual and, for some species, sex, gonad maturity, and the weight of certain organs (stomach, liver, gonads) are also evaluated. The soft rays of the anal fin are counted for redfish and otoliths are collected for redfish and Atlantic cod. Invertebrates are weighted and counted (no individual measurements). The biological data are divided into 4 files: a “Metadata” file containing set information, a “Catches” file containing catches per set for fish taxa, a “Carbio” file containing biological and morphometric measurements per individual and a “Freql” file containing the length frequency of fish. It's important to note that this is raw data. Only sets considered successful are retained. In each set, all species are kept, with a few exceptions. For more information please contact the data management team (gddaiss-dmsaisb@dfo-mpo.gc.ca).