Funded under DFO's Marine Conservation Targets Program, this optical imagery benthic survey captured 66 drift-camera transects from September 22, 2023 to October 3, 2024 in the Fundy Isles region of the lower, western Bay of Fundy, New Brunswick, Canada. The survey area includes the 'Head Harbour/West Isles Archipelago/The Passages' Ecologically and Biologically Significant Area (ESBA, ~113 km2), the Wolves Islands and Grand Manan Island. High-resolution still images (n=4695) were taken periodically throughout each transect, while continuous high-definition downward- and forward-facing video (>28 hours of each) was collected simultaneously. Distance travelled and distance between still images (m) was calculated using ArcGIS tools. Field of view (FOV) was estimated by measuring the length and width of a subset of still images (n=343 in year one and n=520 in year two) in ImageJ2, using 10-cm lasers for scale. FOV was standardized for each reported altitude. Transects ranged from 133 m to 2.6 km in length (~44 km surveyed in total), collecting imagery continuously for 3 minutes to more than 1 hour at a time, surveying depths from 15 to 188 m below chart datum. Transect locations were selected based on unique bathymetric features, areas previously predicted to have high habitat suitability for vulnerable marine ecosystem species, as well as proposed areas for inclusion in the regional marine conservation network plan. Cite this data as: Lawton P, Teed L. Near-seafloor drift transect video and high-resolution digital still imagery from a two-year survey in the Fundy Isles region of the lower, western Bay of Fundy. Published February 2025. Coastal Ecosystems Science Division, Fisheries and Oceans Canada, St. Andrews, N.B.

Funded under DFO's Marine Conservation Targets Program, this optical imagery benthic survey documents the occurrence and estimated percent cover of the invasive colonial tunicate, Didemnum vexillum in seven drift-camera transects in the 'Head Harbour/West Isles Archipelago/The Passages' Ecologically and Biologically Significant Area (ESBA, ~113km2) in the western Bay of Fundy, New Brunswick, Canada. Occurrence data was derived from the use of high-resolution still images (n=386) taken periodically throughout each transect, and simultaneous continuous high-definition video. Video was divided into 20-second segments (here, we report the start and end location of each segment within a transect) and when D. vexillum was present in a video segment, frequency of occurrence was classified as common (continuous coverage/patches throughout the video segment), occasional (individual colonies of various sizes encountered >5 times throughout the video segment), or rare (small, isolated colonies encountered ≤5 times throughout the video segment). A video segment was deemed unusable and removed from the dataset if there was too much turbidity, or if the camera position was too high off-bottom to reliably image the seafloor. For still images, when D. vexillum was observed in an image, colony percent cover was categorized as >50%, 26-50%, 6-25%, or ≤5% of the images field of view (FOV). Distance travelled and distance between still images (m) was calculated using ArcGIS tools. FOV was estimated by measuring the length and width of a subset of still images and video frame grabs in ImageJ2, using 10-cm lasers for scale. FOV was standardized for each reported altitude, and area sampled (m2) along a continuous video segment was estimated by multiplying the average FOV by the distance travelled in that segment. D. vexillum was found in 44% of the area sampled at depths from 34 to 118m, deeper than previous reports globally of ~80m. Cite this data as: Teed LL, Goodwin C, Lawton P, Lacoursière-Roussel A, Dinning KM (2024) Multiple perspectives on the emergence of the invasive colonial tunicate Didemnum vexillum Kott, 2002 in the western Bay of Fundy, Atlantic Canada. BioInvasions Records 13(3): 713–738, https://doi.org/10.3391/bir.2024.13.3.12

Funded under DFO's Marine Conservation Targets Program, this two-year optical imagery benthic survey captured 41 drift-camera transects in the St. Anns Bank Marine Protected Area (MPA - 4364 km2) and 4 coastal transects west of the MPA, Atlantic Canada from August 15-23, 2023 and August 08-17, 2024. High-resolution still images (n=3605) were taken periodically throughout each transect, while continuous high-definition downward- and forward-facing video (~20 hours of each) was collected simultaneously along with over 14 hours of forward-facing Go-Pro video (only in 2024). Transect and image locations were collected using an acoustic positioning operating system (Kongsberg APOS) acquired in 2024; year-one of the survey relied on the vessel position. Distance travelled and distance between still images (m) was calculated using ArcGIS tools. Field of view (FOV) was estimated by measuring the length and width of a subset of still images from year-one of the survey (n=500) in ImageJ2, using 10-cm lasers for scale. FOV was standardized for each reported altitude. Transects ranged from 319 m to 2.8 km in length (~47 km surveyed in total), collecting imagery for 12 minutes to just over 1 hour at a time, surveying depths from 17 to 160 m below chart datum. Transect locations were selected based on unique bathymetric features and benthoscapes as well as areas previously surveyed from 2009-2015. Cite this data as: Lawton P, Teed L. Near-seafloor drift transect video imagery and high-resolution digital still images from a two-year survey in support of Marine Protected Area monitoring of St. Anns Bank, Atlantic Canada. Published January 2025. Coastal Ecosystems Science Division, Fisheries and Oceans Canada, St. Andrews, N.B.

Funded under DFO's Marine Conservation Targets Program, this optical imagery benthic survey captured 16 drift-camera transects in the St. Anns Bank Marine Protected Area (MPA - 4364 km2) and 2 coastal transects west of the MPA, Atlantic Canada from August 15-23, 2023. High-resolution still images (n=1941) were taken periodically throughout each transect, while continuous high-definition downward- (~9 hours) and forward-facing (~8.5 hours) video was collected simultaneously. Distance travelled and distance between still images (m) was calculated using ArcGIS tools. Field of view (FOV) was estimated by measuring the length and width of a subset of still images (n=500) in ImageJ2, using 10-cm lasers for scale. FOV was standardized for each reported altitude. Transects ranged from 754 m to 2.8 km in length (~22 km surveyed in total), collecting imagery for 20 minutes to just over 1 hour at a time, surveying depths from 17 to 144 m below chart datum. Transect locations were selected based on unique bathymetric features and benthoscapes as well as areas previously surveyed in 2015. Cite this data as: Lawton P, Teed L. Near-seafloor drift transect video imagery and high-resolution digital still images from a 2023 survey in support of Marine Protected Area monitoring of St. Anns Bank, Atlantic Canada. Published September 2024. Coastal Ecosystems Science Division, Fisheries and Oceans Canada, St. Andrews, N.B.

We evaluated an autonomous environmental DNA sampler produced by Dartmouth Ocean Technologies Inc (Dartmouth, Canada) compared to time-at-sample filtration in the laboratory to determine the performance of moored samplers for monitoring in the marine world. We deployed three autonomous samplers from DOT in the Bedford Basin (Canada) over a nine-week period in summer/fall 2023. The samplers filtered seawater in situ at programmed interviews over this time period, and we collected contemporaneous samples with a standard vacuum pump during each sampling period. Both eDNA sample types captured similar fish diversity, including typical diversity for the Northwest Atlantic. The invertebrate community detected using the COI marker was different between each sample type, likely due to differences in filter pore size. We found biofouling on the moored samplers was minimal over the study period, even in a high-traffic area such as the Bedford Basin, likely due to the relatively short experimental period, and copper screening covering in the inlet and outlet valves of the instruments. Overall, our results show promise to deploy autonomous eDNA samplers in marine conservation areas to contribute to monitoring in the temperate ocean, but further testing over longer periods of time is needed to determine if DNA remains well-preserved in the autonomous samplers at ambient ocean temperatures. Cite this data as: Jeffery, N.W., Van Wyngaarden, M., and Stanley, R.R.E. Evaluating an Autonomous eDNA Sampler for Marine Environmental Monitoring: Short- and Long-Term Applications. Published: December 2024. Coastal Ecosystems Science Division, Maritimes Region, Fisheries and Oceans Canada, Dartmouth NS.

To assess the current distribution of kelp beds and other macroalgae in Nova Scotia and Southwest New Brunswick, subtidal drop camera surveys were performed from 2022-2023 at 140 sites. For each site, a GoPro HERO 10 camera was towed along a deep (7-12m) and shallow (3-5m) depth contour until 20 images were acquired per depth. Where possible, species were identified from photos, with particular care given to kelps (defined here as orders Laminariales and Tilopteridales) and fucoids (order Fucales). Crust-forming algae was not counted. Percent cover was calculated using a 10x10 point grid overlaid on each image and recording the dominant cover type at each point. The depth (in meters) of each photo after correcting for tide height ranged from ~0.5m to ~12m. Depths were corrected to chart datum (lowest astronomical tide) using tide predictions from the nearest tide station, taken from tides.gc.ca. Sampling was performed between July and October. Cite this data as: Krumhansl K, DiBacco C (2024). Camera Surveys of the Subtidal Flora of Nova Scotia and Southwest New Brunswick 2022-2023. Version 1.5. Fisheries and Oceans Canada. Samplingevent dataset. https://ipt.iobis.org/obiscanada/resource?r=camera_surveys_of_the_subtidal_flora_of_nova_scotia_2022-2023&v=1.5 For additional information please see: Krumhansl K.A., Brooks C.M., Lowen B., O’Brien J., Wong M., DiBacco C. Loss, resilience and recovery of kelp forests in a region of rapid ocean warming. Annals of Botany 2024 Mar 8; 133(1):73-92. Brooks C.M., Krumhansl K.A. 2023. First record of the Asian Antithamnion sparsum Tokida, 1932 (Ceramiales, Rhodophyta) in Nova Scotia, Canada. BioInvasions Records 12(3):745-725.