Survey for Physella wright - the hotwater physa, at Liard River Hotsprings Provincial Park, August 2006. Description of activity: The research is a survey of the Hotwater physa, Physella wrighti, to estimate population distribution and abundance, in order to monitor the population of this freshwater snail found in a single hot spring site in Canada. Researchers will gather data regarding the density of snail populations and the characteristics of the habitat it utilizes in order to provide an updated assessment of its status. The proposed methodology allows accurate, monitoring of this population. Estimates of snail density per square meter will be calculated based on repeated sweeps of vegetation to dislodge snails. Where snails are found on open substrate, counts are done by quadrat. Attempts will be made to document egg case deposition. Population density estimates and ecosystem data will be sampled for every meter of stream where P. wrighti is known to occur. Each sample site will be georeferenced and documented using digital photography.

Atlantic sturgeon. Important sites: reproduction, feeding juvenile and adult concentration area. Data extracted from the Fish Habitat Management Information System (FHAMIS) according to a literature review of documents produced between 1978 and 2002.

Survey data depicting the presence of the endangered Rocky Mountain Ridged Mussel (Gonidea angulata) from 2008-2011. Surveys were conducted by different researchers at different locations.

Description: Chlorophyll-a concentration (a proxy for phytoplankton biomass) was retrieved from the MODIS instrument on the Aqua satellite, with data distributed by the NASA Ocean Biology Processing Group, and averaged into monthly climatological composites. The data span the years 2003-2020 and this record includes data at 4 km pixel resolution. Methods: MODIS-Aqua Chlorophyll-a (Chl-a) was acquired from the NASA Ocean Biology Processing Group at processing Level-3 (version 2018), 4-km resolution, where Chl-a concentration was calculated using the OC3/OCI method. The months of January and December were excluded from this dataset, as data in the winter months at higher latitudes are missing due to low sun angle preventing acquisition. The monthly geometric mean value at all pixels was calculated for individual years, then the geometric mean and geometric standard deviation factor of chlorophyll-a were calculated by month from these images. These methods of calculating mean and standard deviation were used due to the log-normal distribution of chlorophyll-a. The geometric standard deviation is a unitless factor, where the lower bound is the ratio of the geometric mean and geometric standard deviation, and the upper bound is the multiplication of the two. In addition to the geometric mean and geometric standard deviation factor the number of occurrences of valid data at each pixel over the period of observation were calculated. Pixels with fewer than two occurrences over the entire period of observation were removed from these maps, and set to a NaN value in the tif files. All resulting rasters were cropped to the Canadian Exclusive Economic Zone and assigned to the NAD83 geographic coordinate reference system (EPSG:4269), and have a final pixel resolution of approximately 0.0417 degrees. The monthly geometric mean, monthly geometric standard deviation factor, and number of occurrences for all pixels are provided. Data Sources: NASA Ocean Biology Processing Group. (2017). MODIS-Aqua Level 2 Ocean Color Data Version R2018.0. NASA Ocean Biology Distributed Active Archive Center. https://doi.org/10.5067/AQUA/MODIS/L2/OC/2018 Uncertainties: Satellite values have been evaluated against global datasets, and datasets of samples in the Pacific region (see references). However, uncertainties are introduced when averaging together images over time as each pixel has a differing number of observations. Short-lived or spatially limited events may be missed.

Lake sturgeon's presence zones, breeding, feeding and concentration areas according to a literature review of documents produced between 1976 and 2002.Data extracted from the Fish Habitat Management Information System (FHAMIS).

Important sites: reproduction, feeding and concentration area. Data extracted from the Fish Habitat Management Information System (FHAMIS) according to a literature review of documents produced between 1976 and 1999.

Feeding areas, breeding areas, concentration and specie present. Data extracted from the Fish Habitat Management Information System (FHAMIS) according to a literature review of documents produced between between 1977 and 2001

Atlantic herring. Concentration, breeding areas and larval retention areas of Atlantic Herring in the St. Lawrence Estuary.Data extracted from the Fish Habitat Management Information System (FHAMIS). Concentration areas, spawning sites and breeding areas of Atlantic herring according to a literature review of documents produced between 1977 and 1998.

CMIST is a peer-reviewed screening-level risk assessment tool for marine invasive species (Drolet et al. 2016). It is a short questionnaire that follows the invasion process from arrival to impact and is designed so an informed assessor can evaluate one species in an assessment area in approximately one day using easily accessible information from internet databases, primary literature, and grey literature. Species can be those with an invasion history in an area or those that are candidates for future invasions. CMIST is score-based and incorporates both likelihood and impact of invasion as well as uncertainty. Questions are general to make CMIST broadly applicable to different taxa, different assessment areas, and different project goals. To date, CMIST has been tested with molluscs, tunicates, crustaceans, and polychaetes introduced or at risk of introduction to three Canadian marine ecoregions (DFO 2009). CMIST has also been successfully applied to non-indigenous freshwater fish in British Columbia with adapted guidelines (T. Therriault, pers. comm.). Upon completion, CMIST produces a risk score adjusted for the assessor’s uncertainty which, combined with information collected during the assessment, can be used to assist in management decisions. For example, in 2015, CMIST assessments were used to identify high risk invaders in three Canadian marine ecoregions (DFO 2016).

Greenland Sharks (Somniosus microcephalus) are estimated to have the highest longevity of any invertebrate (392 ± 120 years), making bycatch a significant concern (Nielsen et al. 2016). However, in the Newfoundland and Labrador (NL) region, accurate estimates of bycatch are not often available for the species (Simpson et al. 2021). To address this, species distribution models (SDMs) were generated to delineate habitat suitability for the species throughout the NL region in order to identify areas where a higher rate of bycatch is expected to occur. Observations of Greenland shark bycatch recorded by At-Sea Observers (ASOs) in NL (1983 – 2019), Spain (1999 – 2017), and by the Northwest Atlantic Fisheries Organization (NAFO) Secretariat (2014 – 2019) were compiled to generate a presence-only dataset. Multiple environmental variables were assessed for collinearity, and non-collinear variables (Bathymetry and mean monthly bottom temperature for March and November (1990 – 2015)) were retained for use in the SDM. MaxEnt (maximum entropy) software was used to model habitat suitability because it is a presence-only modelling program that is able to account for a lack of absence data by comparing the environmental conditions at occurrence locations to those at randomly selected background points. Overall, the results indicated that habitat suitability for Greenland Shark was highest in deeper waters along the shelf edge in NAFO Divisions 3OP, and the Laurentian Channel, but also extended along the edge of the Labrador shelf, the Grand Banks, and deeper areas along the continental shelf such as the Hawke Channel, Funk Island Deep, and the slopes of Saglek, Nain, and Hamilton Banks. Beyond the Economic Exclusive Zone (EEZ) and within the NAFO regulatory area (NRA), suitable habitat was also present within the Flemish Pass, and along the slope of the Flemish Cap and shelf edge in NAFO Divisions 3NO (Simpson et al. 2021). More detailed information can be found in Simpson et al. 2021. References: Nielsen, J., R. B. Hedeholm, J. Heinemeier, P. G. Bushnell, J. S. Christiansen, J. Olsen, C. B. Ramsey, R. W. Brill, M. Simon, K. F. Steffensen, J. F. Steffensen. 2016. Eye lens radiocarbon reveals centuries of longevity in the Greenland shark (Somniosus microcephalus). Science 353 (6300):702-704 Simpson, M. R., Gullage, L., Konecny, C., Ollerhead, N., Treble, M.A., Nogueira, A., González-Costas, F. 2021. Spatial-temporal variation in Greenland shark (Somniosus microcephalus) bycatch in the NAFO Regulatory Area. NAFO SCR Doc. 21/028