The federal Whales Protection Initiative began in 2018. The first phase of this five‑year initiative focused primarily on the Southern Resident Killer Whale (Orcinus orca). The second phase of the initiative, which took place from April 2023 to March 2026, made it possible to allocate additional resources to support efforts targeting the St. Lawrence Estuary Beluga (Delphinapterus leucas). The threats identified in the Recovery Program for the St. Lawrence Estuary Beluga population under the Species at Risk Act (SARA) include, among others, habitat loss and disturbance, reduced prey abundance, quality, and availability, human‑induced disturbance, and the presence of contaminants. (https://www.canada.ca/fr/environnement-changement-climatique/services/registre-public-especes-peril/programmes-retablissement/beluga-population-estuaire-saint-laurent/chapitre-2.html#s1_5) As part of Initiative 2.0, the team responsible for monitoring and water quality surveillance at Environment and Climate Change Canada (ECCC) conducted sampling of freshwater, sediments, and biota at the mouths of major freshwater tributaries flowing into the beluga’s critical habitat. This sampling program aimed to document the concentrations and detection frequency of contaminants considered to be of concern. The team also sought to test a multi‑matrix approach by collecting water, sediment, and biota samples at the same locations and at the same time. The sampling sites are located around Québec City (St. Lawrence River, Boyer River, Saint‑Charles River, Sainte‑Anne‑de‑Beaupré River, Aulneuse River, Etchemin River), within the critical habitat area (Tadoussac, Moulin‑à‑Baude River, Sainte‑Marguerite River, Mars River, Essipit), as well as within the area proposed for critical habitat expansion (Rivière‑du‑Loup, Isle‑Verte River, Cacouna, Trois‑Pistoles River, Bic Park). The lists of sampling sites and targeted contaminants were adjusted as the program evolved. Sampling frequency was established based on the matrices.

The Canada Nature Fund for Aquatic Species at Risk (CNFASAR) is a contribution program that focuses on providing funding for recovery and threat mitigation activities in nine priority places and to address two marine threats to aquatic species at risk. The Priority Places and Marine Threats layer supports CNFASAR by delineating the location of the places and threats. The Canada Nature Fund for Aquatic Species at Risk (CNFASAR) supports applicants in the design and delivery of stewardship projects. These projects support the recovery and protection of aquatic species at risk. DFO has identified 2 priority marine threats and 9 priority places as the focus for projects funded by CNFASAR, these areas are included in this dataset.