PURPOSE: This product serves a public facing webpage for the Canadian public to download Atlantic Bluefin Tuna stomach content data. DESCRIPTION: Metadata and stomach content from fish caught in the commercial fishery. SAMPLING METHODS: Stomachs were collected from Atlantic Bluefin Tuna (ABFT) caught from mid-August to late September over six years (2018–2023). Most samples originated from ABFT caught around the eastern end of Prince-Edward Island, which reflects the dominant ABFT fishing area, while a few samples were obtained from the Miscou/Baie-des-Chaleurs area in 2018 and 2019. Fish were measured to the nearest curved fork length (cm) and weighed to the nearest round weight (kg). Stomachs were obtained directly from harvesters or through a fish buyer and were stored at −20 ◦C before being processed in the laboratory. Stomachs identification numbers were cross-referenced with ABFT tag numbers recorded by fish provider in order to obtain logbook and port data (catch location, time, weight length, sex, gear, etc.) for each sample. Stomachs were thawed in the laboratory and the content was sorted and identified to the lowest possible taxonomic level. For each stomach, prey were weighed collectively as a taxonomic group and individually to the nearest 0.1 g. Dead bait used to capture ABFT, identified by cut marks, were recorded and weighed but excluded from the analysis. Live bait items cannot be identified from stomach content analyses. Only a few otoliths were found in 2018 and their degraded quality precluded performing ageing or species identification. Rare and small prey items such as algae and rocks were classified in the category “other”. Fish remains that could not be identified were classified in the category “Unidentified teleostei remains”. For 2019 to 2023, when stomach content items could not be visually identified and when tissue was available, tissue samples were collected and stored at −20 °C for DNA barcoding analysis. DNA extraction, mitochondrial cytochrome oxidase subunit 1 amplification, Sanger sequencing and species assignation were performed at the Plateforme d’Analyses Génomiques and Plateforme Bio-informatique of the Institut de Biologie Intégrative et des Systèmes (PAG-IBIS, Université Laval, Quebec city, QC, Canada, http://www.ibis.ulaval.ca/en/services-2/genomic-analysis-platform/). DNA was extracted from 20 mg of muscle tissue using the Omega Bio-tek E-Z-96 Tissue DNA Kit (Omega Bio-tek, Norcross GA, USA) following manufacturer instructions. The mitochondrial cytochrome oxidase subunit 1 region was amplified and sequenced as described in Hashemzadeh Segherloo et al., 2021). Sanger forward and reverse reads were analyzed independently using the Basic Local Alignment Search Tool against non-redundant sequences to identify the top hit for each sequence. When samples could not be identified by a top hit sequence they were classified as “unidentifiable fish”. Prey items that were successfully identified using DNA barcoding were incorporated into the stomach content analysis database and used in all subsequent diet analyses (abundance, occurrence and weight). The weight of the items used in the database was the weight of the remains as they were, and not reconstructed weights calculated for a live animal of the species identified by the barcoding. USE LIMITATION: To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.

PURPOSE: To characterize food webs of lake fish communities using stable isotopes, gut content morphology and DNA. DESCRIPTION: Data sets containing fish species caught in Miramichi Lake, McKiel Lake and Nashwaak Lake. USE LIMITATION: To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.

PURPOSE: To characterize food webs of freshwater fish communities using stable isotopes DESCRIPTION: Data set containing the carbon, nitrogen, and sulphur stable isotope ratios of fishes caught in the Northwest Miramichi River system PARAMETERS COLLECTED: Species gut contents (ecological); species isotopic signatures (ecological); points (spatial) PHYSICAL SAMPLE DETAILS: Powdered animal tissue SAMPLING METHODS: Invertebrates were sampled via kicknet and were collected 1-4 meters from the shore of each location in one-minute intervals with 5-10 intervals per site. Fish were captured using a variety of net types in order to maximize sampling breadth. Please see below for list of net types and mesh sizes: Large fyke (box, lead line, and cod = 5mm tight, 10mm loose); 6mm beach seine; Site 3: two small fyke nets (box, lead line, and cod = 17mm, 17mm and 10mm tight, and 32mm, 32mm, 20mm loose), one trap net (box = 47.6mm and lead line = 146mm) were used). USE LIMITATION: To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.

To better understand patterns of temporal and spatial variation of fish assemblages in offshore waters of Lake Winnipeg (Manitoba, Canada), pelagic trawl tows were conducted at lakewide monitoring stations since 2002. Trawl samples collected during spring, summer, and fall from the south basin, channel, and north basin were used to study effects of season and geographic region within the lake on species biomass estimates.

PURPOSE: In this study, we examined the structure and function of the Southampton Island marine food web across 149 species of benthic and pelagic invertebrates, fishes, marine mammals and seabirds collected from 2016 to 2019, to provide a baseline for future studies that aim to quantify temporal changes in food web structuring. More specifically,we used a multi-biomarker approach combining stable isotopes and HBIs to: (i) determine the vertical trophic structure of the marine food web, (ii) investigate the contribution of benthic and pelagic-derived prey to the higher trophic level species of the Arctic food web, and (iii) determine the role of ice algae and phytoplankton carbon source use across different trophic levels and compartments (pelagic and benthic). By shedding new light on the functioning of the Southampton Island food web and specifically how the contribution of ice algae and benthic habitat shapes its structure, these results will be relevant to adaptive management and conservation initiatives implemented in response to anthropogenic stressors and climate change. DESCRIPTION: Climate-driven alterations of the marine environment are most rapid in Arctic and subarctic regions, including Hudson Bay in northern Canada, where declining sea ice, warming surface waters and ocean acidification are occurring at alarming rates. These changes are altering primary production patterns that will ultimately cascade up through the food web. Here, we investigated (i) the vertical trophic structure of the Southampton Island marine ecosystem in northern Hudson Bay, (ii) the contribution of benthic and pelagic-derived prey to the higher trophic level species, and (iii) the relative contribution of ice algae and phytoplankton derived carbon in sustaining this ecosystem. For this purpose, we measured bulk stable carbon, nitrogen and sulfur isotope ratios as well as highly branched isoprenoids in samples belonging to 149 taxa, including invertebrates, fishes, seabirds and marine mammals. We found that the benthic invertebrates occupied 4 trophic levels and that the overall trophic system went up to an average trophic position of 4.8. The average δ34S signature of pelagic organisms indicated that they exploit both benthic and pelagic food sources, suggesting there are many interconnections between these compartments in this coastal area. The relatively high sympagic carbon dependence of Arctic marine mammals (53.3 ± 22.2 %) through their consumption of benthic invertebrate prey, confirms the important role of the benthic subweb for sustaining higher trophic level consumers in the coastal pelagic environment. Therefore, a potential decrease in the productivity of ice algae could lead to a profound alteration of the benthic food web and a cascading effect on this Arctic ecosystem. Collaborators: Centre for Earth Observation Science, University of Manitoba, Winnipeg, Manitoba, Canada - R´emi Amiraux, C.J. Mundy, Jens K. Ehn, Z.A. Kuzyk. Quebec-Ocean, Sentinel North and Takuvik, Biology Department, Laval University, Quebec, Quebec, Canada - Marie Pierrejean. Scottish Association for Marine Science, Oban, UK - Thomas A. Brown. Department of Natural Resource Sciences, McGill University, Ste. Anne de Bellevue, Quebec, Canada - Kyle H. Elliott. Department of Biological Sciences, University of Manitoba, Winnipeg, Manitoba, Canada - Steven H. Ferguson, Cory J.D. Matthews, Cortney A. Watt, David J. Yurkowski. School of the Environment, University of Windsor, Windsor, Ontario, Canada - Aaron T. Fisk. Science and Technology Branch, Environment and Climate Change Canada, Ottawa, Ontario, Canada - Grant Gilchrist. College of Fisheries and Ocean Sciences, University of Alaska Fairbanks, Fairbanks, AK, USA - Katrin Iken. Department of Earth Sciences, University of New Brunswick, Fredericton, NB, Canada - Audrey Limoges. Department of Integrative Biology, University of Windsor, Windsor, Ontario, Canada - Oliver P. Love, Wesley R. Ogloff. Department of Arctic Biology, The University Centre in Svalbard, Longyearbyen, Norway - Janne E. Søreide.

"Fall" missions occur primarily in October and November, but sets from September and December are also present in the data. Collected data includes total catch in numbers and weights by species. Length frequency data is available for most species, as are the age, sex, maturity and weight information for a subset of the individual animals. Other data such as ageing material, genetic material, and stomach contents are often also collected, but are stored elsewhere. "Fall" cruises occur in September, October, November and December. Cite this data as: Clark, D., Emberley, J. Data of Maritimes Fall Research Vessel Survey. Published January 2021. Population Ecology Division, Fisheries and Oceans Canada, Dartmouth, N.S. https://open.canada.ca/data/en/dataset/5f82b379-c1e5-4a02-b825-f34fc645a529

The Fisheries and Oceans Canada ecosystem surveys are conducted annually and are a source of integrated ecosystem monitoring data. These survey data are the primary data source for monitoring trends in species distribution, abundance, and biological condition within the region, and also provide data to the Atlantic Zonal Monitoring Program (AZMP) for monitoring hydrographic conditions, along with zooplankton and phytoplankton. The surveys follow a stratified random sampling design, and include sampling using a bottom otter trawl, CTD rosette and vertical plankton tows. Data from the bottom trawl catch are used to monitor the distribution and abundance of fish and invertebrates throughout the Scotian Shelf, Bay of Fundy and Georges Bank. Cite this data as: Clark, D., Emberley, J. Data of MARITIMES RESEARCH VESSEL SURVEYS. Published January 2021. Population Ecology Division, Fisheries and Oceans Canada, Dartmouth, N.S. https://open.canada.ca/data/en/dataset/8ddcaeea-b806-4958-a79f-ba9ab645f53b

“4VSW” missions focus on the eastern half of the Scotian Shelf, and occur primarily in March, but sets in both February, and April are also present in the data. These missions use a unique stratification scheme intended to optimize the abundance estimates of cod. Collected data includes total catch in numbers and weights by species. Length frequency data is available for most species, as are the age, sex, maturity and weight information for a subset of the individual animals. Other data such as ageing material, genetic material, and stomach contents are often also collected, but are stored elsewhere. Cite this data as: Clark, D., Emberley, J. Data of Maritimes 4VSW Research Vessel Survey. Published January 2021. Population Ecology Division, Fisheries and Oceans Canada, Dartmouth, N.S. https://open.canada.ca/data/en/dataset/a851ce30-e216-4d7d-a29c-05631eef140e

The Fisheries and Oceans Canada (DFO) ecosystem surveys consist of research vessel survey data collected to monitor the distribution and abundance of fish and invertebrates throughout the Scotian Shelf, Bay of Fundy and Georges Bank. The surveys follow a stratified random sampling design, and include sampling of fish and invertebrates using a bottom otter trawl. These survey data are the primary data source for monitoring trends in species distribution, abundance, and biological condition within the region, and also provide data to the Atlantic Zonal Monitoring Program (AZMP) for monitoring hydrographic variability. Collected data includes total catch in numbers and weights by species. Length frequency data is available for most species, as are the age, sex, maturity and weight information for a subset of the individual animals. Other data such as ageing material, genetic material, and stomach contents are often also collected, but are stored elsewhere. “Spring” cruises occur in January, February, March and April, and focus on Georges Bank (i.e. 5Z). Cite this data as: Clark, D., Emberley, J. Data of MARITIMES SPRING RESEARCH VESSEL SURVEY. Published January 2021. Population Ecology Division, Fisheries and Oceans Canada, Dartmouth, N.S. https://open.canada.ca/data/en/dataset/fecf045a-95a2-4b69-8a40-818649a62716

“Summer” missions occur in June, July and August and these focus on the Scotian Shelf and Bay of Fundy (i.e. 4VWX 5Yb, expanding recently to include the Laurentian Channel and Georges Bank (5Zc). Collected data includes total catch in numbers and weights by species. Length frequency data is available for most species, as are the age, sex, maturity and weight information for a subset of the individual animals. Other data such as ageing material, genetic material, and stomach contents are often also collected, but are stored elsewhere. “Summer” cruises occur in May, June, July and August and these focus on the Scotian Shelf and Bay of Fundy (i.e. 4VWX). Cite this data as: Clark, D., Emberley, J. Data of MARITIMES SUMMER RESEARCH VESSEL SURVEYS. Published January 2021. Population Ecology Division, Fisheries and Oceans Canada, Dartmouth, N.S. https://open.canada.ca/data/en/dataset/1366e1f1-e2c8-4905-89ae-e10f1be0a164