Bay-scale empirical demonstrations of how bivalve aquaculture alters plankton composition, and subsequently ecological functioning and higher trophic levels, are lacking. Temporal, inter- and within-bay variation in hydrodynamic, environmental, and aquaculture pressure limit efficient plankton monitoring design to detect bay-scale changes and inform aquaculture ecosystem interactions. Here, we used flow cytometry to investigate spatio-temporal variations in bacteria and phytoplankton (< 20 µm) composition in four bivalve aquaculture embayments. We observed higher abundances of bacteria and phytoplankton in shallow embayments that experienced greater freshwater and nutrient inputs. Depleted nutrient conditions may have led to the dominance of picophytoplankton cells, which showed strong within-bay variation as a function of riverine vs freshwater influence and nutrient availability. Although environmental forcings appeared to be a strong driver of spatio-temporal trends, results showed that bivalve aquaculture may reduce near-lease phytoplankton abundance and favor bacterial growth. We discuss aquaculture pathways of effects such as grazing, benthic-pelagic coupling processes, and microbial biogeochemical cycling. Conclusions provide guidance on optimal sampling considerations using flow cytometry in aquaculture sites based on embayment geomorphology and hydrodynamics. Cite this data as: Sharpe H, Lacoursière-Roussel A, Barrell J (2024). Monitoring bay-scale bivalve aquaculture ecosystem interactions using flow cytometry. Version 1.2. Fisheries and Oceans Canada. Samplingevent dataset. https://ipt.iobis.org/obiscanada/resource?r=monitoring_bay-scale_bivalve_aquaculture_ecosystem_interactions_using_flow_cytometry&v=1.2

To support the surveillance of key macroalgae and non-indigenous species in Nova Scotia and New Brunswick, five quantitative PCR (qPCR) assays were designed and tested at 111 sites in 2022-2023 targeting the following non-indigenous macroalgal species: Antithamnion sparsum, Bonnemaisonia hamifera, Codium fragile, Dasysiphonia japonica, Fucus serratus. All assays were developed in 2022 by the Center for Environmental Genomics Applications (CEGA, Newfoundland, Canada) except Antithamnion sparsum, for which an assay was developed in 2023 by the Aquatic Biotechnology Laboratory (ABL) at the Bedford Institute of Oceanography. All amplification was performed by the ABL in 2022-2023. The assay developed for Fucus serratus was later determined to be non-specific, and amplifies both F. serratus and Fucus distichus. Cite this data as: Krumhansl K, Brooks C, Lowen B, DiBacco C, (2025). Quantitative PCR (qPCR) of Key Macroalgal Non-Indigenous Species in Nova Scotia and New Brunswick Waters. Version 1.5. Fisheries and Oceans Canada. Samplingevent dataset. https://ipt.iobis.org/obiscanada/resource?r=quantitative_qpcr_macroalgal_nonindigenous_species_novascotia_newbrunswick_2022_2023&amp;v=1.5 For additional information please see: LeBlanc F., Belliveau V., Watson E., Coomber C., Simard N., DiBacco C., Bernier R., Gagné N. 2020. Environment DNA (eDNA) detection of marine aquatic invasive species (AIS) in Eastern Canada using a targeted species-specific qPCR approach. Management of Biological Invasions 11(2):201-217 Krumhansl K.A., Brooks C.M., Lowen B., O’Brien J., Wong M., DiBacco C. Loss, resilience and recovery of kelp forests in a region of rapid ocean warming. Annals of Botany 2024 Mar 8; 133(1):73-92 Brooks C.M., Krumhansl K.A. 2023. First record of the Asian Antithamnion sparsum Tokida, 1932 (Ceramiales, Rhodophyta) in Nova Scotia, Canada. BioInvasions Records 12(3):745-725.

GIS compilation of data used to perform the stacked cumulative chance of success (resource potential map) in Open file 8556. Natural Resources Canada (NRCan) has been tasked, under the Marine Conservation Targets (MCT) initiative announced in Budget 2016, with evaluating the petroleum resource potential for areas identified for possible protection as part of the Government of Canada's commitment to conserve 10% of its marine areas by 2020. As part of this initiative, NRCan's Geological Survey of Canada (GSC) conducted a broad regional study of the petroleum potential over the majority of the Magdalen Basin, which is the principal geological basin in the southern Gulf of St. Lawrence. The GSC resource assessment is visually represented by a qualitative petroleum potential map. Disclaimer: A simplified colored version of the map is displayed on the Web Mapping Service (WMS). The correct version is available for download through the Federal Geospatial Platform (FGP) and GEOSCAN.

“Kouchibouguac National Park - Total Ecosystem Forest Carbon Density” is the annual carbon density (tonnes carbon per hectare) within Kouchibouguac’s forested ecosystems over a 31-year period from 1990 to 2020. Total Ecosystem Forest Carbon Density includes aboveground and belowground biomass, soil carbon, and dead organic matter. Total Ecosystem Forest Carbon Density was estimated for 31 national parks using the Generic Carbon Budget Model (GCBM), a spatially explicit carbon budget model developed by Canadian Forest Service which uses forest inventory, disturbance, and mean annual temperature data along with yield data to estimate growth and merchantable volume for dominant tree species. Species- and Ecozone-specific equations are then used to convert merchantable volume to aboveground and belowground biomass carbon. Ecozones were classified according to Canada Ecological Land Classification Level 1. The GCBM simulates carbon dynamics to produce spatially explicit estimations of carbon stocks and fluxes. The model simulates and tracks carbon stocks, transfers between Intergovernmental Panel on Climate Change-defined pools, and other metrics including net ecosystem production, net biome production, and emissions of carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O) in annual time steps. The stocks and fluxes are also tracked by disturbance event (e.g., forest fires, insect outbreaks). Total Ecosystem Forest Carbon Density accounts for the effects of natural and anthropogenic disturbances, including wildfires, prescribed burns, and insect outbreaks. These products have a spatial resolution of 30m. This information is part of the Parks Canada Carbon Atlas Series. To obtain a copy of this report, please contact changementclimatique-climatechange@pc.gc.ca. When using this data, please cite as follows: Sharma, T., Kurz, W.A., Fellows, M., MacDonald, A.L., Richards, J., Chisholm, C., Seutin, G., Richardson, K., Keenleyside, K. (2023). Parks Canada Carbon Atlas Series: Carbon Dynamics in the Forests of Canada’s National Parks. Scientific Report. Parks Canada Agency, Gatineau, QC, Canada, 104 p.

“Fundy National Park – Total GHG Emissions” datasets consist of estimates of GHG emissions (carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O)) in carbon dioxide equivalents (CO2e) from forested ecosystems in Fundy National Park from 1990 to 2020 (tonnes carbon dioxide equivalent per hectare). Total GHG emissions for 31 national parks were estimated using the Generic Carbon Budget Model (GCBM), a spatially explicit carbon budget model developed by Canadian Forest Service which uses forest inventory, disturbance, and mean annual temperature data along with yield data to estimate growth and merchantable volume for dominant tree species. Species- and Ecozone-specific equations are then used to convert merchantable volume to aboveground and belowground biomass carbon. The GCBM simulates carbon dynamics to produce spatially explicit estimations of carbon stocks and fluxes. The model simulates and tracks carbon stocks, transfers between Intergovernmental Panel on Climate Change (IPCC)-defined pools, and other metrics including net ecosystem production, net biome production, and emissions of carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O) in annual time steps. The stocks and fluxes are also tracked by disturbance event (e.g., forest fires). Total GHG emissions include those from natural processes like respiration and decomposition and those due to natural and anthropogenic disturbances, including wildfires, prescribed burns, and insect outbreaks. These were calculated as the sum of CO2, CH4, and N2O emission estimates in tonnes carbon (tonnes C) generated by the GCBM. Emissions estimates were then converted to carbon dioxide equivalents (CO2e) using the 100-year Global Warming Potential (IPCC Fourth Assessment Report) factors for CH4 (25) and N2O (298). These products have a spatial resolution of 30m. This information is part of the Parks Canada Carbon Atlas Series. To obtain a copy of this report, please contact changementclimatique-climatechange@pc.gc.ca. When using this data, please cite as follows: Sharma, T., Kurz, W.A., Fellows, M., MacDonald, A.L., Richards, J., Chisholm, C., Seutin, G., Richardson, K., Keenleyside, K. (2023). Parks Canada Carbon Atlas Series: Carbon Dynamics in the Forests of Canada’s National Parks. Scientific Report. Parks Canada Agency, Gatineau, QC, Canada, 104 p.

“Fundy National Park - Total Ecosystem Forest Carbon Density” is the annual carbon density (tonnes carbon per hectare) within Fundy’s forested ecosystems over a 31-year period from 1990 to 2020. Total Ecosystem Forest Carbon Density includes aboveground and belowground biomass, soil carbon, and dead organic matter. Total Ecosystem Forest Carbon Density was estimated for 31 national parks using the Generic Carbon Budget Model (GCBM), a spatially explicit carbon budget model developed by Canadian Forest Service which uses forest inventory, disturbance, and mean annual temperature data along with yield data to estimate growth and merchantable volume for dominant tree species. Species- and Ecozone-specific equations are then used to convert merchantable volume to aboveground and belowground biomass carbon. Ecozones were classified according to Canada Ecological Land Classification Level 1. The GCBM simulates carbon dynamics to produce spatially explicit estimations of carbon stocks and fluxes. The model simulates and tracks carbon stocks, transfers between Intergovernmental Panel on Climate Change-defined pools, and other metrics including net ecosystem production, net biome production, and emissions of carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O) in annual time steps. The stocks and fluxes are also tracked by disturbance event (e.g., forest fires, insect outbreaks). Total Ecosystem Forest Carbon Density accounts for the effects of natural and anthropogenic disturbances, including wildfires, prescribed burns, and insect outbreaks. These products have a spatial resolution of 30m. This information is part of the Parks Canada Carbon Atlas Series. To obtain a copy of this report, please contact changementclimatique-climatechange@pc.gc.ca. When using this data, please cite as follows: Sharma, T., Kurz, W.A., Fellows, M., MacDonald, A.L., Richards, J., Chisholm, C., Seutin, G., Richardson, K., Keenleyside, K. (2023). Parks Canada Carbon Atlas Series: Carbon Dynamics in the Forests of Canada’s National Parks. Scientific Report. Parks Canada Agency, Gatineau, QC, Canada, 104 p.

Fisheries and Oceans Canada’s (DFO) Coastal Environmental Baseline Program supports the collection of ecological information on the current state of key coastal ecosystems across Canada. This initiative aims to acquire environmental baseline data (physical, chemical and biological) contributing to the characterization of important coastal areas and to support evidence-based assessments and management decisions for preserving marine ecosystems. From this page, you will find links to the data from projects undertaken from 2018-2022 at six coastal sites across Canada.

“Kouchibouguac National Park - Total GHG Emissions” datasets consist of estimates of GHG emissions (carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O)) in carbon dioxide equivalents (CO2e) from forested ecosystems in Kouchibouguac National Park from 1990 to 2020 (tonnes carbon dioxide equivalent per hectare). Total GHG emissions for 31 national parks were estimated using the Generic Carbon Budget Model (GCBM), a spatially explicit carbon budget model developed by Canadian Forest Service which uses forest inventory, disturbance, and mean annual temperature data along with yield data to estimate growth and merchantable volume for dominant tree species. Species- and Ecozone-specific equations are then used to convert merchantable volume to aboveground and belowground biomass carbon. The GCBM simulates carbon dynamics to produce spatially explicit estimations of carbon stocks and fluxes. The model simulates and tracks carbon stocks, transfers between Intergovernmental Panel on Climate Change (IPCC)-defined pools, and other metrics including net ecosystem production, net biome production, and emissions of carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O) in annual time steps. The stocks and fluxes are also tracked by disturbance event (e.g., forest fires). Total GHG emissions include those from natural processes like respiration and decomposition and those due to natural and anthropogenic disturbances, including wildfires, prescribed burns, and insect outbreaks. These were calculated as the sum of CO2, CH4, and N2O emission estimates in tonnes carbon (tonnes C) generated by the GCBM. Emissions estimates were then converted to carbon dioxide equivalents (CO2e) using the 100-year Global Warming Potential (IPCC Fourth Assessment Report) factors for CH4 (25) and N2O (298). These products have a spatial resolution of 30m. This information is part of the Parks Canada Carbon Atlas Series. To obtain a copy of this report, please contact changementclimatique-climatechange@pc.gc.ca. When using this data, please cite as follows: Sharma, T., Kurz, W.A., Fellows, M., MacDonald, A.L., Richards, J., Chisholm, C., Seutin, G., Richardson, K., Keenleyside, K. (2023). Parks Canada Carbon Atlas Series: Carbon Dynamics in the Forests of Canada’s National Parks. Scientific Report. Parks Canada Agency, Gatineau, QC, Canada, 104 p.

Most of the data were collected during aerial surveys carried out at low tides during June and August 1994-1997, 2000 and 2001. June and August are respectively pupping and moulting seasons, when the haulout sites are intensively used by seals. Features in this layer show the Harbour seal distribution and the mean abundance for all aerial surveys (tables 3 and 5, figures 3 and 5 from Robillard et al. 2005). In the estuary, areas of high abundance have more than 30 individuals, areas of medium abundance have between 10 and 30 individuals and areas of low abundance have fewer than 10 individuals. In the Gulf, areas of high abundance have more than 50 individuals and areas of medium to low abundance have fewer than 50 individuals. Unpublished data obtained from Parks Canada and Sepaq were also used to identify important haulout areas in the Saguenay Fjord sector and in Pointe-aux-Vaches tidal flat sectors, which have been categorized in this dataset as high abundance areas. Data are valid only during summer (except for the Pointe-aux-Vaches flats identified as mainly frequented in autumn by Parc Canada), because spring and fall distributions of the Harbour seal are unknown. Data shown in the Estuary and the Gulf of St. Lawrence are a picture of the situation in 2005 because it is the most recent mapping available for this specie. The distribution of the Harbour seal is non-uniform among the different concentration areas but is similar between June and August. However, Harbour seals tend to decrease their presence along the south shore and the Lower Estuary in August to the benefit of the Saguenay River colonies. Abundance classes are arbitrary but fit with the published results of haulout site utilization from Robillard et al. (2005). Data sources : Parks Canada. 2021. Personal communication. Harbor seal monitoring data on the Pointe-aux-Vaches tidal flat. Parks Canada and SÉPAQ, 2020. Données du suivi du phoque commun dans le fjord du Saguenay. Unpublished data. Robillard, A., V. Lesage, and M.O. Hammill. 2005. Distribution and abundance of harbour seals (Phoca vitulina concolor) and grey seals (Halichoerus grypus) in the Estuary and Gulf of St. Lawrence, 1994–2001. Can. Tech. Rep. Fish. Aquat. Sci. 2613: 152 pp.