PURPOSE: This product serves a public facing webpage for the Canadian public to download Atlantic Bluefin Tuna stomach content data. DESCRIPTION: Metadata and stomach content from fish caught in the commercial fishery. SAMPLING METHODS: Stomachs were collected from Atlantic Bluefin Tuna (ABFT) caught from mid-August to late September over six years (2018–2023). Most samples originated from ABFT caught around the eastern end of Prince-Edward Island, which reflects the dominant ABFT fishing area, while a few samples were obtained from the Miscou/Baie-des-Chaleurs area in 2018 and 2019. Fish were measured to the nearest curved fork length (cm) and weighed to the nearest round weight (kg). Stomachs were obtained directly from harvesters or through a fish buyer and were stored at −20 ◦C before being processed in the laboratory. Stomachs identification numbers were cross-referenced with ABFT tag numbers recorded by fish provider in order to obtain logbook and port data (catch location, time, weight length, sex, gear, etc.) for each sample. Stomachs were thawed in the laboratory and the content was sorted and identified to the lowest possible taxonomic level. For each stomach, prey were weighed collectively as a taxonomic group and individually to the nearest 0.1 g. Dead bait used to capture ABFT, identified by cut marks, were recorded and weighed but excluded from the analysis. Live bait items cannot be identified from stomach content analyses. Only a few otoliths were found in 2018 and their degraded quality precluded performing ageing or species identification. Rare and small prey items such as algae and rocks were classified in the category “other”. Fish remains that could not be identified were classified in the category “Unidentified teleostei remains”. For 2019 to 2023, when stomach content items could not be visually identified and when tissue was available, tissue samples were collected and stored at −20 °C for DNA barcoding analysis. DNA extraction, mitochondrial cytochrome oxidase subunit 1 amplification, Sanger sequencing and species assignation were performed at the Plateforme d’Analyses Génomiques and Plateforme Bio-informatique of the Institut de Biologie Intégrative et des Systèmes (PAG-IBIS, Université Laval, Quebec city, QC, Canada, http://www.ibis.ulaval.ca/en/services-2/genomic-analysis-platform/). DNA was extracted from 20 mg of muscle tissue using the Omega Bio-tek E-Z-96 Tissue DNA Kit (Omega Bio-tek, Norcross GA, USA) following manufacturer instructions. The mitochondrial cytochrome oxidase subunit 1 region was amplified and sequenced as described in Hashemzadeh Segherloo et al., 2021). Sanger forward and reverse reads were analyzed independently using the Basic Local Alignment Search Tool against non-redundant sequences to identify the top hit for each sequence. When samples could not be identified by a top hit sequence they were classified as “unidentifiable fish”. Prey items that were successfully identified using DNA barcoding were incorporated into the stomach content analysis database and used in all subsequent diet analyses (abundance, occurrence and weight). The weight of the items used in the database was the weight of the remains as they were, and not reconstructed weights calculated for a live animal of the species identified by the barcoding. USE LIMITATION: To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.

Aquatic invasive species pose economic and ecological threats to Canada's coastal waters. In response, Fisheries and Oceans Canada (DFO) has established monitoring programs to detect and track the spread of aquatic invasive species, including European Green Crab, in Canadian waters. Fukui traps have been deployed annually at both new and long-term monitoring locations throughout coastal British Columbia.

The Coastal Biodiversity Trawl Survey for the Passamaquoddy Bay was conducted annually between July to October from 2009 to 2019. This survey was intended to monitor long-term change in local species presence, habitat utilization, and health. The sampling activities support coastal research in fisheries, aquaculture, marine protected areas, and ecosystem change. Data collected prior to 2013 are generally not recommended for comparative analysis due to changes in vessel, sampling effort, and protocols.

Ichthyoplankton surveys were conducted in the Strait of Georgia (British Columbia) during 1979-1981 to ascertain the onset of fish spawning, and to explore distributional pattern and estimate total biomass of fish species. Oblique tows were made using 0.25m2 Bongos equipped with 351 micron Nitex nets of modified SCOR design. All sampling gear was black to minimize potential avoidance and resulting catch bias. The tow procedure generally followed that established by CALCOFI. This dataset contains a compilation of corrected catches of juvenile fishes, fish eggs and fish larvae by station.

The Department of Fisheries established a survey of demersal juvenile Atlantic cod (*Gadus morhua*) in the nearshore (<10 m deep) in 1959. This survey aimed to characterize the distribution and abundance of juvenile Atlantic cod and was based upon Norway's Flodevigen sampling program which has been conducted continuously since 1919. A 25 m seine was used to sample juvenile Atlantic cod nursery locations on the Avalon Peninsula and Northeast coast of the island of Newfoundland in September and October. The survey continued until 1964 and became known as the Fleming survey, after original initiator Alistair Fleming. The survey was reinstated by Memorial University of Newfoundland from 1992 to 1997. Multiple tows were conducted at a subset of the original 55 Fleming sites located in St. Mary's Bay, Trepassey Bay, the Southern Shore, Conception Bay, Trinity Bay, Bonavista Bay, Gander Bay, New World Island, Fortune Harbour, Badger Bay, Halls Bay and Green Bay. This data set includes several different subsets, some of which span both Fleming Survey periods (1959-1964 and 1992-1997): I. JuvCodCatch60s90s: Catches of juvenile Atlantic cod (1959-1964, 1992-1997) from the first two consecutive tows at each site. This is a summary based on JuvCodLengths90s and FlemingSurveyData60s; II. JuvCodLength90s: Lengths of all individual juvenile Atlantic cod caught for each site (1992-1997); III. SiteEnvData60s90s: Station data for Fleming data 1992-1997; IV. FlemingSurveyData60s: Fleming survey data from 1959-1964 (note there are three record types pertaining to: station data "type 1"; general species catch data including juvenile Atlantic cod grouped by age class ("1+", "Zeroes", and "Total") "type 2; and juvenile Atlantic cod (species 223) listed in length bins "type 3"; and V. FlemingBycatch60s92to96: Bycatch data from 1959-1964 & 1992-1996 from first two tows at each site. This is a summary based partly on the FlemingSurveyData60s set.

Polygons denoting concentrations of sea pens, small and large gorgonian corals and sponges on the east coast of Canada have been identified through spatial analysis of research vessel survey by-catch data following an approach used by the Northwest Atlantic Fisheries Organization (NAFO) in the Regulatory Area (NRA) on Flemish Cap and southeast Grand Banks. Kernel density analysis was used to identify high concentrations and the area occupied by successive catch weight thresholds was used to identify aggregations. These analyses were performed for each of the five biogeographic zones of eastern Canada. The largest sea pen fields were found in the Laurentian Channel as it cuts through the Gulf of St. Lawrence, while large gorgonian coral forests were found in the Eastern Arctic and on the northern Labrador continental slope. Large ball-shaped Geodia spp. sponges were located along the continental slopes north of the Grand Banks, while on the Scotian Shelf a unique population of the large barrel-shaped sponge Vazella pourtalesi was identified. The latitude and longitude marking the positions of all tows which form these and other dense aggregations are provided along with the positions of all tows which captured black coral, a non-aggregating taxon which is long-lived and vulnerable to fishing pressures. These polygons identify sea pen fields from the broader distribution of seapens in the region as sampled by Cosmos trawl gear in the Eastern Arctic biogeographic zone. A 0.1 kg minimum threshold for the sea pen catch was identified as the weight that separated the sea pen field habitat from the broader distribution of sea pens with these research vessel tow data and gear type.

Polygons denoting concentrations of sea pens, small and large gorgonian corals and sponges on the east coast of Canada have been identified through spatial analysis of research vessel survey by-catch data following an approach used by the Northwest Atlantic Fisheries Organization (NAFO) in the Regulatory Area (NRA) on Flemish Cap and southeast Grand Banks. Kernel density analysis was used to identify high concentrations and the area occupied by successive catch weight thresholds was used to identify aggregations. These analyses were performed for each of the five biogeographic zones of eastern Canada. The largest sea pen fields were found in the Laurentian Channel as it cuts through the Gulf of St. Lawrence, while large gorgonian coral forests were found in the Eastern Arctic and on the northern Labrador continental slope. Large ball-shaped Geodia spp. sponges were located along the continental slopes north of the Grand Banks, while on the Scotian Shelf a unique population of the large barrel-shaped sponge Vazella pourtalesi was identified. The latitude and longitude marking the positions of all tows which form these and other dense aggregations are provided along with the positions of all tows which captured black coral, a non-aggregating taxon which is long-lived and vulnerable to fishing pressures. These polygons identify sea pen fields from the broader distribution of seapens in the region as sampled by Campelen trawl gear in the Eastern Arctic biogeographic zone. A 0.05 kg minimum threshold for the sea pen catch was identified as the weight that separated the sea pen field habitat from the broader distribution of sea pens with these research vessel tow data and gear type.

Polygons denoting concentrations of sea pens, small and large gorgonian corals and sponges on the east coast of Canada have been identified through spatial analysis of research vessel survey by-catch data following an approach used by the Northwest Atlantic Fisheries Organization (NAFO) in the Regulatory Area (NRA) on Flemish Cap and southeast Grand Banks. Kernel density analysis was used to identify high concentrations and the area occupied by successive catch weight thresholds was used to identify aggregations. These analyses were performed for each of the five biogeographic zones of eastern Canada. The largest sea pen fields were found in the Laurentian Channel as it cuts through the Gulf of St. Lawrence, while large gorgonian coral forests were found in the Eastern Arctic and on the northern Labrador continental slope. Large ball-shaped Geodia spp. sponges were located along the continental slopes north of the Grand Banks, while on the Scotian Shelf a unique population of the large barrel-shaped sponge Vazella pourtalesi was identified. The latitude and longitude marking the positions of all tows which form these and other dense aggregations are provided along with the positions of all tows which captured black coral, a non-aggregating taxon which is long-lived and vulnerable to fishing pressures. These polygons identify large gorgonian coral fields from the broader distribution of large gorgonian corals in the region as sampled by Western II A trawl gear in the Scotian Shelf biogeographic zone. A 0.5 kg minimum threshold for the large gorgonian coral catch was identified as the weight that separated the large gorgonian field habitat from the broader distribution of large gorgonian corals with these research vessel tow data and gear type.

This dataset contains digital data files on transects flown and reported in Harwood, L.A. and P. Norton (1996). Aerial survey data from the southeast Beaufort Sea, Mackenzie River estuary and west. Amundsen Gulf, July 1992. Canadian Data Report of Fisheries and Aquatic Sciences No. 964

Polygons denoting concentrations of sea pens, small and large gorgonian corals and sponges on the east coast of Canada have been identified through spatial analysis of research vessel survey by-catch data following an approach used by the Northwest Atlantic Fisheries Organization (NAFO) in the Regulatory Area (NRA) on Flemish Cap and southeast Grand Banks. Kernel density analysis was used to identify high concentrations and the area occupied by successive catch weight thresholds was used to identify aggregations. These analyses were performed for each of the five biogeographic zones of eastern Canada. The largest sea pen fields were found in the Laurentian Channel as it cuts through the Gulf of St. Lawrence, while large gorgonian coral forests were found in the Eastern Arctic and on the northern Labrador continental slope. Large ball-shaped Geodia spp. sponges were located along the continental slopes north of the Grand Banks, while on the Scotian Shelf a unique population of the large barrel-shaped sponge Vazella pourtalesi was identified. The latitude and longitude marking the positions of all tows which form these and other dense aggregations are provided along with the positions of all tows which captured black coral, a non-aggregating taxon which is long-lived and vulnerable to fishing pressures. These polygons identify sea pen fields from the broader distribution of seapens in the region as sampled by Alfredo trawl gear in the Eastern Arctic biogeographic zone. A 0.25 kg minimum threshold for the sea pen catch was identified as the weight that separated the sea pen field habitat from the broader distribution of sea pens with these research vessel tow data and gear type.