Species characterization by environmental DNA (eDNA) is a method that allows the use of DNA released into the environment by organisms from various sources (secretions, faeces, gametes, tissues, etc.). It is a complementary tool to standard sampling methods for the identification of biodiversity. This project provides a list of fish and marine mammal species whose DNA has been detected in water samples collected between 2019 and 2021 using the mitochondrial marker MiFish (12S). The surveys were carried out in the summer of 2019 (July 14-18) and (July 30 - August 5), in the fall of 2020 (October 27-28) and in the summer-fall of 2021 (May 31 - June 3 ) and (August 24-25) between Forestville and Godbout (Haute-Côte-Nord). Sampling was carried out between 1-50 meters depth in 91 stations, with 1 to 3 replicates per station. Two liters of water were filtered through a 1.2 µm fiberglass filter. DNA extractions were performed with the DNeasy Blood and Tissues or PowerWater extraction kit (Qiagen). Negative field, extraction and PCR controls were added at the different stages of the protocol. The libraries were prepared either by Génome Québec (2019, 2020) or by the Genomics Laboratory of the Maurice-Lamontagne Institute (2021), then sequenced on a NovaSeq 4000 PE250 system by Génome Québec. The bioinformatics analysis of the sequences obtained was carried out using an analysis pipeline developed in the genomics laboratory. A first step made it possible to obtain a table of molecular operational taxonomic units (MOTU) using the cutadapt software for the removal of the adapters and the R package DADA2 for the filtration, the fusion, removal of chimeras and compilation of data. The MOTUs table was then corrected using the R package metabaR to eliminate the tag-jumping and take contaminants into consideration. Samples showing a strong presence of contaminating MOTUs were removed from the dataset. The MOTUs were also filtered to remove all remaining adapter sequences and also retain only those of the expected size (around 170 bp). Finally, taxonomic assignments were made on the MOTUs using the BLAST+ program and the NCBI-nt database. Taxonomic levels (species, genus or family) were assigned using a best match method (Top hit), with a threshold of 95%. Only assignments at the level of fish and marine mammals were considered, and the taxa detected were compared to a list of regional species, and corrected if necessary. The species detections of the different replicas have been combined. The file provided includes generic activity information, including site, station name, date, marker type, assignment types used for taxa identification, and a list of taxa or species. The list of taxa has been verified by a biodiversity expert from the Maurice-Lamontagne Institute. This project was funded by Fisheries and Oceans Canada's Coastal Environmental Baseline Data Program under the Oceans Protection Plan. This initiative aims to acquire baseline environmental data that contributes to the characterization of significant coastal areas and supports evidence-based assessments and management decisions to preserve marine ecosystems. Data were also published on SLGO platform : https://doi.org/10.26071/ogsl-2239bca5-c24a

A goal of the Government of Canada’s Coastal Environmental Baseline Program (CEBP) is to amalgamate historic environmental data from high vessel traffic areas. An extensive DFO biological sampling program was conducted from 1980 to 1981 on Sturgeon and Roberts banks located on the outer Fraser River estuary, BC, Canada. This report collates and simplifies three data sets: water quality and nearshore fish catch previously published as DFO Data Report 340 (Conlin et al.1982), and un- published weight-length (W-L) data for two locations on Sturgeon Bank (Iona and Steveston) and near Westshore Terminals (Coal Port) on Roberts Bank. W-L data were reconstructed from archived computer printouts using Optical Character Recognition methods. Analyses of water quality data indicate that the two banks provided different fish habitats with Sturgeon Bank having a greater freshwater influence. Although Iona area water quality was exposed to sewage outfall from a nearby sewage treatment plant, it appears that fish communities were not different from the other Sturgeon Bank area (Steveston). The fish communities were found to be different between the two banks with Roberts Bank having greater overall abundance and diversity. Interestingly, of the seven fish species used for condition factor analyses, five were found to have lower Relative Condition Factors in the Roberts Bank sampling area.

From August 6th to September 9th, 2014, Fisheries and Oceans Canada conducted a baseline survey of marine fishes and their habitats on the Canadian Beaufort Shelf and slope. Sampling was conducted from the F/V Frosti at 56 stations along thirteen transects, with an additional three stations not located on transects. Standardized sampling was conducted on the transects at pre-determined depth stations (20-40, 75, 200, 350, 500, 750, and 1000 m) using a variety of sampling equipment including benthic fishing trawls, plankton nets, sediment cores, and CTD and water sample profiles. Presented here is the information on the sampling locations, and the sampling gear deployed at each station.

This dataset contains observations of species occurrences from seafloor imagery collected by the autonomous underwater vehicle (AUV) during the 2012 Expedition to Cobb Seamount. The National Oceanographic and Atmospheric Administration-operated SeaBED-class AUV which collected photographic images from 4 transects ranging from 436 m to 1154 m in depth.

Summary The Quebec region of the Department of Fisheries and Oceans (DFO) is responsible for the assessment of several fish and invertebrate stocks exploited in the Estuary and the northern Gulf of St. Lawrence. The commercial catches sampling program is one of the sources of information used to complete these assessments. The data collected by this program, at wharf or at sea, offers among other things the advantage of a relatively large spatio-temporal coverage and provides some of the necessary knowledge to assess the demography and the structure of the exploited populations. This program is implemented by specialized DFO staff whose main mandate is to collect biological data on groundfish, pelagic fish and marine invertebrate species that are commercially exploited in the various marine communities. Data This dataset on the Atlantic cod (Gadus morhua) includes the metadata, sample weight, fish length, the sex and the number of specimens measured. This dataset covers the period of 1983 to present. In order to protect the confidentiality of the sources, some informations (such as those concerning the vessel) have been excluded and others (such as the date of capture) have been simplified. Entries where there was only one vessel in a fishing area for a given year were also excluded. Further information including the fishing areas coordinates can be found by clicking on the «Atlantic and Arctic commercial fisheries» and «Fishing areas» links below.

Cold-water corals are conspicuous in the waters off Eastern Canada. Despite that, there are few DNA sequence records from specimens collected in the region available in GenBank, and not all species recorded in the region have sequence data regardless of geographic origin. This can limit the use of eDNA techniques to detect and identify corals. Our objective was to sequence and publish sequences for two octocoral DNA barcoding markers: CO1 and MutS. We sequenced and deposited 36 sequences to GenBank from 19 specimens representing three sea pen taxa (Octocorallia: Pennatuloidea): Distichoptilum gracile, Pennatula aculeata, and Protoptilum carpenteri. Identification of all specimens was confirmed by B. M. Neves before submission. Specimens and DNA tissues were donated to the Canadian Museum of Nature, where they are currently stored. This publication is part 1 of a series of GenBank submissions by our lab. Specimens were collected from across the Northwest Atlantic and originate from depths ranging between 200-1924 meters. Specimens were collected as part of research vessel multispecies trawl surveys or remotely operated vehicle (ROV ROPOS) surveys. DNA was isolated and purified using the QIAgen DNeasy Blood and Tissue kit, with an initial overnight incubation with Proteinase K. Two commonly used octocoral barcoding regions were amplified using previously described primers: 1) COII8068F (McFadden et al., 2004) and COIOCTR (France and Hoover, 2002) for the CO1 gene, and 2) ND42599F (France and Hoover, 2002) and mut3458R (Sánchez et al., 2003) for the MutS gene. Amplifications were conducted using 12.5 µl of Green DreamTaq Master Mix (Thermo Fisher Scientific), 1 µl of template DNA, 0.5 µl of each 10 µM forward and reverse primers, 0.5 µl of 10 µM reverse primer, and 10.5 µl of water. Thermocycling was run as follows: 3 min of initial denaturation at 95 °C, followed by 40 cycles at 95 °C for 30 s, 30 s at annealing temperature of 48 °C, then 65 s at an extension temperature of 72 °C, and a final elongation at 72 °C for 4 min. PCR products were cleaned using Agencourt AMPure XP Beads (Beckman Coulter) and sent to The Center for Advanced Genomics, Toronto, Canada for Sanger sequencing. Sequences were visualized and aligned using Geneious Prime 2022.0.2. Obtained sequences have been deposited in GenBank under accession numbers OQ569768- OQ569784 and OQ420359- OQ420377. This work was funded by Fisheries and Oceans Canada under an Enhanced Regional Capacity grant (2020-2021) and the Marine Conservation Targets (MCT) program (2021-2024), Newfoundland and Labrador Region.

Fisheries and Oceans Canada (DFO) has been conducting surface water trawl surveys since 1992 in the coastal waters of British Columbia, Washington, Oregon and Alaska and in the high seas of the Gulf of Alaska. These surveys initially focused on determining the migratory patterns (1992-2002) and on the growth and physiology (2003-2016) of juvenile Pacific Salmon. Data collected in shelf and slope waters at depths less than 400 meters off Washington and Oregon State are part of this larger survey series, but are limited to 1999-2001. These surveys focused on determining the migratory patterns of juvenile Pacific Salmon and had funding support from the Bonneville Power Administration as part of the 1995-2011 Canada-USA Salmon Shelf Survival Study. The intent of that study was to monitor and evaluate the effects of ocean conditions on the distribution, migration, growth, and survival of Pacific salmon during their first ocean year, and estimate the subsequent impacts on abundance of Chinook salmon adults returning to the Columbia River system.

This dataset contains digital data files on transects flown and reported in Harwood, L.A. and P. Norton (1996). Aerial survey data from the southeast Beaufort Sea, Mackenzie River estuary and west. Amundsen Gulf, July 1992. Canadian Data Report of Fisheries and Aquatic Sciences No. 964

PURPOSE: This study is part of a two-decade series of research aimed to provide a comprehensive synthesis of the effects of harvest and environmental change on fisheries in Great Bear Lake. The main objectives are to assess demographic traits and the current status of harvested species, with a focus on evaluating sustainable harvest levels of lake trout, a cold-adapted species with a relatively narrow thermal niche. As part of this research, trends in water quality and primary productivity are monitored to evaluate potential effects of change on fisheries. DESCRIPTION: Great Bear Lake, one of the largest lakes in North America, contains culturally and recreationally important fish species. Great Bear Lake is located in the sub-Arctic and Arctic Circle. As part of a two-decade series of research aimed to provide a comprehensive synthesis of the effects of harvest and environmental change on fisheries in Great Bear Lake, the main objectives of this study are to assess demographic traits and the current status of harvested species, with a focus on evaluating sustainable harvest levels of lake trout, a cold-adapted species with a relatively narrow thermal niche. As part of this research, trends in water quality and primary productivity are monitored to evaluate potential effects of change on fisheries. From 2021 to 2024, surface water temperature data was collected at depths of 0.1 to 1.0 meters using an RBR Maestro3 through partnered community-led and community/Fisheries and Oceans Canada/university partner collaborative sampling. The project has strong community involvement, including youth through the Guardian Program, to facilitate capacity building and community leadership in the long-term monitoring of Great Bear Lake fisheries and the aquatic ecosystem. This data is an extension of baseline data sets on water quality on the lake. These data will contribute to a better understanding cumulative impacts of climate change on the functioning of large northern lake ecosystems and provide a benchmark for monitoring further change. This data will be important for developing effective strategies for maintaining community-led aquatic monitoring and managing natural resources, particularly fish, which are expected to be increasingly important to communities with declines in other country foods such as caribou. We acknowledge the data were collected in the Sahtú Settlement Area and are made publicly available with the agreement of the Délı̨nę Renewable Resources Council (Délı̨nę Ɂehdzo Got’ı̨nę (Renewable Resources Council)). Collaborators include: the Community of Délı̨nę partners (data collection), Délı̨nę Renewable Resource Council, Sahtú Renewable Resource Board, and University of Manitoba. Community of Délı̨nę partners and field workers that participated in data collection include Chris Yukon, Archie Vital, Ted Mackienzo, Daniel Baton, Lloyd Baton, Simon Neyelle, and Stanley Ferdanan. Funding and logistical support was provided by: Northwest Territories Cumulative Impact Monitoring, Sahtú Renewable Resource Board, the Polar Continental Shelf Program and Fisheries and Oceans Canada.

This record contains data on beluga whale locations in the Mackenzie estuary reported in Harwood, L.A. and P. Norton (1996). Aerial survey data from the southeast Beaufort Sea, Mackenzie River estuary and west. Amundsen Gulf, July 1992. Canadian Data Report of Fisheries and Aquatic Sciences No. 964