PURPOSE: Scope the distribution of Smallmouth Bass in the Miramichi River Watershed using eDNA DESCRIPTION: This dataset contains the results of work undertaken from 2019 to 2024 to scope the extent of the spread of Smallmouth Bass in parts of the Miramichi River Watershed using an eDNA-based species-specific qPCR approach. USE LIMITATION: To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.

Cold-water corals are conspicuous in the waters off Eastern Canada. Despite that, there are few DNA sequence records from specimens collected in the region available in GenBank, and not all species recorded in the region have sequence data regardless of geographic origin. This can limit the use of eDNA techniques to detect and identify corals. Our objective was to sequence and publish sequences for two octocoral DNA barcoding markers: CO1 and MutS. We sequenced and deposited 36 sequences to GenBank from 19 specimens representing three sea pen taxa (Octocorallia: Pennatuloidea): Distichoptilum gracile, Pennatula aculeata, and Protoptilum carpenteri. Identification of all specimens was confirmed by B. M. Neves before submission. Specimens and DNA tissues were donated to the Canadian Museum of Nature, where they are currently stored. This publication is part 1 of a series of GenBank submissions by our lab. Specimens were collected from across the Northwest Atlantic and originate from depths ranging between 200-1924 meters. Specimens were collected as part of research vessel multispecies trawl surveys or remotely operated vehicle (ROV ROPOS) surveys. DNA was isolated and purified using the QIAgen DNeasy Blood and Tissue kit, with an initial overnight incubation with Proteinase K. Two commonly used octocoral barcoding regions were amplified using previously described primers: 1) COII8068F (McFadden et al., 2004) and COIOCTR (France and Hoover, 2002) for the CO1 gene, and 2) ND42599F (France and Hoover, 2002) and mut3458R (Sánchez et al., 2003) for the MutS gene. Amplifications were conducted using 12.5 µl of Green DreamTaq Master Mix (Thermo Fisher Scientific), 1 µl of template DNA, 0.5 µl of each 10 µM forward and reverse primers, 0.5 µl of 10 µM reverse primer, and 10.5 µl of water. Thermocycling was run as follows: 3 min of initial denaturation at 95 °C, followed by 40 cycles at 95 °C for 30 s, 30 s at annealing temperature of 48 °C, then 65 s at an extension temperature of 72 °C, and a final elongation at 72 °C for 4 min. PCR products were cleaned using Agencourt AMPure XP Beads (Beckman Coulter) and sent to The Center for Advanced Genomics, Toronto, Canada for Sanger sequencing. Sequences were visualized and aligned using Geneious Prime 2022.0.2. Obtained sequences have been deposited in GenBank under accession numbers OQ569768- OQ569784 and OQ420359- OQ420377. This work was funded by Fisheries and Oceans Canada under an Enhanced Regional Capacity grant (2020-2021) and the Marine Conservation Targets (MCT) program (2021-2024), Newfoundland and Labrador Region.

Summary The Quebec region of the Department of Fisheries and Oceans (DFO) is responsible for the assessment of several fish and invertebrate stocks exploited in the Estuary and the northern Gulf of St. Lawrence. The commercial catches sampling program is one of the sources of information used to complete these assessments. The data collected by this program, at wharf or at sea, offers among other things the advantage of a relatively large spatio-temporal coverage and provides some of the necessary knowledge to assess the demography and the structure of the exploited populations. This program is implemented by specialized DFO staff whose main mandate is to collect biological data on groundfish, pelagic fish and marine invertebrate species that are commercially exploited in the various marine communities. Data This dataset on the yellowtail flounder (Limanda ferruginea) includes the metadata, sample weight, fish length, the sex and the number of specimens measured. This dataset covers the periods of 1992, 1995, 1997-2010, 2012-2013, 2015-2017 and 2019. In order to protect the confidentiality of the sources, some informations (such as those concerning the vessel) have been excluded and others (such as the date of capture) have been simplified. Entries where there was only one vessel in a fishing area for a given year were also excluded. Further information including the fishing areas coordinates can be found by clicking on the «Atlantic and Arctic commercial fisheries» and «Fishing areas» links below.

The Aquatic Invasive Species Surveillance Database is a compilation of fish community and habitat data from DFO’s Aquatic Invasive Species and Invasive Carp Program early detection surveillance efforts in Canadian waters of the Great Lakes basin. Data includes: sampling site location, date, fish species and counts, and associated habitat information. Annual project-specific details including purpose/objectives and study methodology are often reported in the DFO Canadian manuscript report of fisheries and aquatic sciences series.

Summary The Quebec region of the Department of Fisheries and Oceans (DFO) is responsible for the assessment of several fish and invertebrate stocks exploited in the Estuary and the northern Gulf of St. Lawrence. The commercial catches sampling program is one of the sources of information used to complete these assessments. The data collected by this program, at wharf or at sea, offers among other things the advantage of a relatively large spatio-temporal coverage and provides some of the necessary knowledge to assess the demography and the structure of the exploited populations. This program is implemented by specialized DFO staff whose main mandate is to collect biological data on groundfish, pelagic fish and marine invertebrate species that are commercially exploited in the various marine communities. Data This dataset on the green urchin (Strongylocentrotus droebachiensis) includes the metadata, sample weight, the test width and the number of specimens measured. This dataset covers the periods of 1995, 1997-1999 and 2003 to present. In order to protect the confidentiality of the sources, some informations (such as those concerning the vessel) have been excluded and others (such as the date of capture) have been simplified. Entries where there was only one vessel in a fishing area for a given year were also excluded. Further information including the fishing areas coordinates can be found by clicking on the «Atlantic and Arctic commercial fisheries» and «Fishing areas» links below.

With the changing climate conditions, marine traffic along Canada’s coastal regions has increased over the past few decades and the need to improve our state of preparedness for oil-spill-related emergencies is critical. Baseline coastal information, such as shoreline form, substrate, and vegetation type, is required for prioritizing operations, coordinating onsite spill response activities (i.e., Shoreline Cleanup Assessment Technique [SCAT]), and providing information for wildlife and ecosystem management. Between 2011 and 2016, georeferenced high-definition videography and photos were collected for various study sites along the east coast. The study areas include Labrador, Bay of Fundy and Chedabucto Bay in Atlantic Canada. Data was collected during ice-free and low tide conditions (where applicable) between July and September. Low-altitude helicopter surveys were conducted at each study site to capture video of the shoreline characteristics. In addition to acquiring videography, ground-based observations were recorded in several locations for validation. Shoreline segmentation was then carried out by manual interpretation of the oblique videography and the photos aided by ancillary data. This involved splitting and classifying the shoreline vectors based on homogeneity of the upper intertidal zone. Detailed geomorphological information (i.e., shoreline type, substrate, slope, height, accessibility etc.) describing the upper intertidal, lower intertidal, supratidal and backshore zones was extracted from the video and entered into a geospatial database using a customized data collection form. In addition, biological characteristics like biobands, water features, fauna, human use etc. observed along the coast were recorded. The data was also validated through ground observations (when available) and a second interpreter QA (quality analysis) was performed on each dataset to ensure high quality and consistency. The final dataset contains segments ranging in length from 150 metres to 2500 metres. In total, from 2011 to 2016, within the 3 study sites, about 1,850 km of shoreline were mapped.

Summary The Quebec region of the Department of Fisheries and Oceans (DFO) is responsible for the assessment of several fish and invertebrate stocks exploited in the Estuary and the northern Gulf of St. Lawrence. The commercial catches sampling program is one of the sources of information used to complete these assessments. The data collected by this program, at wharf or at sea, offers among other things the advantage of a relatively large spatio-temporal coverage and provides some of the necessary knowledge to assess the demography and the structure of the exploited populations. This program is implemented by specialized DFO staff whose main mandate is to collect biological data on groundfish, pelagic fish and marine invertebrate species that are commercially exploited in the various marine communities. Data This dataset on the giant scallop (Placopecten magellanicus) includes the metadata, shell height and the number of shells (empty or full) for all the specimens measured. This dataset covers the period of 1985 to present. In order to protect the confidentiality of the sources, some informations (such as those concerning the vessel) have been excluded and others (such as the date of capture) have been simplified. Entries where there was only one vessel in a fishing area for a given year were also excluded. Further information including the fishing areas coordinates can be found by clicking on the «Atlantic and Arctic commercial fisheries» and «Fishing areas» links below.

Summary The Quebec region of the Department of Fisheries and Oceans (DFO) is responsible for the assessment of several fish and invertebrate stocks exploited in the Estuary and the northern Gulf of St. Lawrence. The commercial catches sampling program is one of the sources of information used to complete these assessments. The data collected by this program, at wharf or at sea, offers among other things the advantage of a relatively large spatio-temporal coverage and provides some of the necessary knowledge to assess the demography and the structure of the exploited populations. This program is implemented by specialized DFO staff whose main mandate is to collect biological data on groundfish, pelagic fish and marine invertebrate species that are commercially exploited in the various marine communities. Data This dataset on the Atlantic cod (Gadus morhua) includes the metadata, sample weight, fish length, the sex and the number of specimens measured. This dataset covers the period of 1983 to present. In order to protect the confidentiality of the sources, some informations (such as those concerning the vessel) have been excluded and others (such as the date of capture) have been simplified. Entries where there was only one vessel in a fishing area for a given year were also excluded. Further information including the fishing areas coordinates can be found by clicking on the «Atlantic and Arctic commercial fisheries» and «Fishing areas» links below.

The Canadian Environmental Sustainability Indicators (CESI) program provides data and information to track Canada's performance on key environmental sustainability issues. Canada's conserved areas indicators report the amount and proportion of Canada's terrestrial (land and freshwater) and marine area that is recognized as conserved. Well-managed conserved areas are one way to protect wild species and their habitats for present and future generations. Habitat conservation is a measure of human response to the loss of biodiversity and natural habitat. As the area conserved in Canada increases, more lands and waters are withdrawn from direct human development stresses, thereby contributing to biodiversity conservation and improving the health of ecosystems. In turn, healthy ecosystems provide benefits such as clean water, mitigation of climate change, pollination and improved human health. Information is provided to Canadians in a number of formats including: static and interactive maps, charts and graphs, HTML and CSV data tables and downloadable reports. See the supplementary documentation for the data sources and details on how the data were collected and how the indicator was calculated. Canadian Environmental Sustainability Indicators: https://www.canada.ca/environmental-indicators

From August 5th to September 3rd, 2012, Fisheries and Oceans Canada conducted a baseline survey of marine fishes and their habitats on the Canadian Beaufort Shelf and slope. Sampling was conducted from the F/V Frosti at 28 stations along four transects. Standardized sampling was conducted at pre-determined depth stations (20-40, 75, 200, 350, 500, 750, and 1000 m) using a variety of sampling equipment including benthic fishing trawls, plankton nets, sediment cores, and CTD and water sample profiles. A specialized CTD probe (UCTD) was deployed at an additional 30 locations while the ship was underway. Presented here is the information on the sampling locations, and the sampling gear deployed at each station.