PURPOSE: Establishing efficient, non-destructive sampling methods for clam population assessments. DESCRIPTION: In the Gulf of St. Lawrence (GSL) Management Region, clam assessments are uncommon due to limited resources and the labour-intensive nature of sampling clam beds. Furthermore, clam assessments typically rely on destructive sampling that disturbs sediment and removes animals from their habitat. Establishing efficient, non-destructive sampling methods for clam population assessments can reduce the impact of scientific sampling on these habitats and provide for more efficient monitoring. In this study, we tested the idea that visually observing siphon holes on the sediment surface could predict the presence, number, and size of soft-shell clams across different sites in the southern GSL. Siphon holes reasonably predicted the presence, number, and size/biomass of soft-shell clams in most, but not all, sites. Thus, in many habitats in the GSL, siphon holes can be used for population assessments, providing a powerful tool to enhance Science advice to fisheries managers. Data was collected at the following sites: * Maisonnette, Parc Maisonnette, Maisonnette, New Brunswick, Canada * Kouchibouguac, Loggiecroft wharf, Kouchibouguac National Park, New Brunswick, Canada * Shemogue, Amos Point Road, Little Shemogue, New Brunswick, Canada * Powell's Cove, Powell's Point Provincial Park, Little Harbour, Nova Scotia, Canada PARAMETERS COLLECTED: - Clam abundance - Clam biomass (total sample) - Clam size (length, weight) - Siphon hole abundance - Siphon hole size - Siphon hole characterization (i.e., identification of actual clam based on shape) - Seawater temperature - Sediment grain size - Sediment organic content (%) - Sediment relative moisture content (%) NOTES ON QUALITY CONTROL: Original data entry by Jillian Hunt and/or Isabelle Brennan. Data checked and validated prior to analysis by Jeff Clements. Data further checked and validated prior to publication by Amélie Robichaud. PHYSICAL SAMPLE DETAILS: No physical samples retained. - Clam samples returned back to original habitat after measuring and weighing in the field. - Sediment core samples stored in walk-in freezer and discarded after processing and analysis. SAMPLING METHODS: i. Identifying, counting, weighing, and measuring (with calipers) clams ii. Identifying, counting, and measuring (with calipers) clam siphon holes iii. Seawater temperature monitoring via data loggers iv. Sediment grain size, organic content, and moisture content analysis USE LIMITATION: To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.

This record contains data on beluga whale locations in the Beaufort Sea and west Amundsen Gulf reported in Harwood, L.A. and P. Norton (1996). Aerial survey data from the southeast Beaufort Sea, Mackenzie River estuary and west. Amundsen Gulf, July 1992. Canadian Data Report of Fisheries and Aquatic Sciences No. 964

Summary The Quebec region of the Department of Fisheries and Oceans (DFO) is responsible for the assessment of several fish and invertebrate stocks exploited in the Estuary and the northern Gulf of St. Lawrence. The commercial catches sampling program is one of the sources of information used to complete these assessments. The data collected by this program, at wharf or at sea, offers among other things the advantage of a relatively large spatio-temporal coverage and provides some of the necessary knowledge to assess the demography and the structure of the exploited populations. This program is implemented by specialized DFO staff whose main mandate is to collect biological data on groundfish, pelagic fish and marine invertebrate species that are commercially exploited in the various marine communities. Data This dataset on the Atlantic mackerel (Scomber scombrus) includes the metadata, sample weight, fish length, the sex and the number of specimens measured. This dataset covers the periods of 1976-1977 and 1984 to present. In order to protect the confidentiality of the sources, some informations (such as those concerning the vessel) have been excluded and others (such as the date of capture) have been simplified. Entries where there was only one vessel in a fishing area for a given year were also excluded. Further information including the fishing areas coordinates can be found by clicking on the «Atlantic and Arctic commercial fisheries» and «Fishing areas» links below.

The Aquatic Invasive Species Surveillance Database is a compilation of fish community and habitat data from DFO’s Aquatic Invasive Species and Invasive Carp Program early detection surveillance efforts in Canadian waters of the Great Lakes basin. Data includes: sampling site location, date, fish species and counts, and associated habitat information. Annual project-specific details including purpose/objectives and study methodology are often reported in the DFO Canadian manuscript report of fisheries and aquatic sciences series.

Cold-water corals are conspicuous in the waters off Eastern Canada. Despite that, there are few DNA sequence records from specimens collected in the region available in GenBank, and not all species recorded in the region have sequence data regardless of geographic origin. This can limit the use of eDNA techniques to detect and identify corals. Our objective was to sequence and publish sequences for two octocoral DNA barcoding markers: CO1 and MutS. We sequenced and deposited 36 sequences to GenBank from 19 specimens representing three sea pen taxa (Octocorallia: Pennatuloidea): Distichoptilum gracile, Pennatula aculeata, and Protoptilum carpenteri. Identification of all specimens was confirmed by B. M. Neves before submission. Specimens and DNA tissues were donated to the Canadian Museum of Nature, where they are currently stored. This publication is part 1 of a series of GenBank submissions by our lab. Specimens were collected from across the Northwest Atlantic and originate from depths ranging between 200-1924 meters. Specimens were collected as part of research vessel multispecies trawl surveys or remotely operated vehicle (ROV ROPOS) surveys. DNA was isolated and purified using the QIAgen DNeasy Blood and Tissue kit, with an initial overnight incubation with Proteinase K. Two commonly used octocoral barcoding regions were amplified using previously described primers: 1) COII8068F (McFadden et al., 2004) and COIOCTR (France and Hoover, 2002) for the CO1 gene, and 2) ND42599F (France and Hoover, 2002) and mut3458R (Sánchez et al., 2003) for the MutS gene. Amplifications were conducted using 12.5 µl of Green DreamTaq Master Mix (Thermo Fisher Scientific), 1 µl of template DNA, 0.5 µl of each 10 µM forward and reverse primers, 0.5 µl of 10 µM reverse primer, and 10.5 µl of water. Thermocycling was run as follows: 3 min of initial denaturation at 95 °C, followed by 40 cycles at 95 °C for 30 s, 30 s at annealing temperature of 48 °C, then 65 s at an extension temperature of 72 °C, and a final elongation at 72 °C for 4 min. PCR products were cleaned using Agencourt AMPure XP Beads (Beckman Coulter) and sent to The Center for Advanced Genomics, Toronto, Canada for Sanger sequencing. Sequences were visualized and aligned using Geneious Prime 2022.0.2. Obtained sequences have been deposited in GenBank under accession numbers OQ569768- OQ569784 and OQ420359- OQ420377. This work was funded by Fisheries and Oceans Canada under an Enhanced Regional Capacity grant (2020-2021) and the Marine Conservation Targets (MCT) program (2021-2024), Newfoundland and Labrador Region.

Commercial and exploratory fisheries for Arctic Char, Salvelinus alpinus, provide significant economic opportunities for Nunavummiut in several Nunavut communities. Having an accurate understanding of the weight of the Arctic Char that are harvested is important for tracking harvest and for understanding how biological parameters may be changing over time as a result of exploitation and/or climactic and environmental changes. Unfortunately, most fish enter the processing plants as dressed (gills and viscera removed) and therefore conversion factors have to be applied to reconcile whole (round) weight from dressed weight. Here, we provide an updated conversion factor based on linear regression for Arctic Char from the Halokvik River (locally known as 30 Mile) near the community of Cambridge Bay. This conversion factor can be used moving forward as the Halokvik River continues to be commercially harvested.

Each summer, environmental community groups collect important data to determine if groupings of fish, shrimp and crab – what is called a community- can be used as an indicator of the health status of bays and estuaries. Sampling was conducted from May through September for the first years then from June through August. In 2018 and 2019, the sampling was conducted just once in each estuary. Community group members and staff sample six stations once a month in their designated estuary. Fish, shrimps and crabs are collected with a beach seine net and later released live back to the water once identified and counted. From this, the community groups provide important information to Fisheries and Oceans Canada, including: - identification and numbers of fish, shrimp and crab species; - water conditions and samples; - information on aquatic plants; - sediment samples. With this information, Fisheries and Oceans Canada scientists working with government agencies and universities can conduct analyses to determine the suitability of indicators to assess the health of bays and estuaries. PARAMETERS COLLECTED: Parameters: abundance, species richness, species developmental stage (young-of-the-year or adult), water temperature, water salinity, water dissolved oxygen, dissolved inorganic nutrient (nitrate, nitrite, phosphate), sediment % organic content, sediment % humidity content and sediment mean grain size, % submerged aquatic vegetation cover NOTES ON QUALITY CONTROL: Data entry into Excel and first quality control verification is done by CAMP summer students. A second quality control verification is done by DFO staff. See publ # 2823 attached to this record. In 2018, the historical data was migrated into a relationship database. From this year on, annual data will be entered into the database using a custom application. The application front end has numerous QC elements built-in. SAMPLING METHODS: Please see the following URL for sampling details: http://www.dfo-mpo.gc.ca/Library/319437.pdf

These data sets provide information pertaining to abundant taxa including bottom-dwelling shrimp and fish along trawling and trapping transects in Simoom Sound at November, 2000, and February, 2001. Data sets were compiled and formatted by Meagan Mak. Abstract from report: This study is a component of a larger project designed to compare the effects of shrimp trawling and trapping gear on shrimp, fish and the benthic habitat of Simoom Sound located in Broughton Archipelago, British Columbia, Canada. Otter-trawling, beam-trawling, and trapping took place in three distinct experiment blocks of the central seabed of the Sound. In turn, each block consisted of replicate transects, where a towed submersible video-camera surveyed shrimp and fish before and after trawling. Video surveys were deployed only before trapping. From the video surveys, we determined the abundance of common shrimp taxa and fish.

These data sets provide information pertaining to shrimp and bycatch estimates associated with otter-trawling and trapping (November, 2000) and beam-trawling (February, 2001) in Simoom Sound. Data sets were compiled and formatted by Meagan Mak. Abstract from report: As part of a project investigating possible modification of marine ecosystems by shrimp trawling and trapping, we obtained information on catches offish, shrimp, prawns , and bycatch organisms as well as weight, sex ratios , egg location and colIateral damage to several species of pandalids and eualids. Focusing on the humpback shrimp (Pandalus hypsinotus), we assessed damage to the rostrum, carapace, abdomen, and tail fan on specimens caught by beam trawling, otter trawling, and trapping. Data are given from a preliminary study conducted in Northumberland Channel in June 2000 and more comprehensive sampling from Simoom Sound in November 2000 and February 2001.

This dataset provides projected 30-year, 50-year, and 100-year return levels for harbours in British Columbia by 2050 and 2100 under an intermediate emission scenario SSP245, relative to the mean sea level over 1993-2020. The return levels are a combination of estimated present extreme sea levels and projected mean sea level rise. The present extreme sea levels are derived from hourly coastal sea levels for the period from 1993 to 2020, simulated using a high-resolution Northeast Pacific Ocean Model (NEPOM). The projected mean sea level rise is derived from the regional mean sea level rise data of the IPCC 6th Assessment Report under SSP245, adjusted for the local vertical land motion.