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Species characterization by environmental DNA (eDNA) is a method that allows the use of DNA released into the environment by organisms from various sources (secretions, faeces, gametes, tissues, etc.). It is a complementary tool to standard sampling methods for the identification of biodiversity. This project provides a list of fish and marine mammal species whose DNA has been detected in water samples collected between 2019 and 2021 using the mitochondrial marker MiFish (12S). The surveys were carried out in the summer of 2019 (July 14-18) and (July 30 - August 5), in the fall of 2020 (October 27-28) and in the summer-fall of 2021 (May 31 - June 3 ) and (August 24-25) between Forestville and Godbout (Haute-Côte-Nord). Sampling was carried out between 1-50 meters depth in 91 stations, with 1 to 3 replicates per station. Two liters of water were filtered through a 1.2 µm fiberglass filter. DNA extractions were performed with the DNeasy Blood and Tissues or PowerWater extraction kit (Qiagen). Negative field, extraction and PCR controls were added at the different stages of the protocol. The libraries were prepared either by Génome Québec (2019, 2020) or by the Genomics Laboratory of the Maurice-Lamontagne Institute (2021), then sequenced on a NovaSeq 4000 PE250 system by Génome Québec. The bioinformatics analysis of the sequences obtained was carried out using an analysis pipeline developed in the genomics laboratory. A first step made it possible to obtain a table of molecular operational taxonomic units (MOTU) using the cutadapt software for the removal of the adapters and the R package DADA2 for the filtration, the fusion, removal of chimeras and compilation of data. The MOTUs table was then corrected using the R package metabaR to eliminate the tag-jumping and take contaminants into consideration. Samples showing a strong presence of contaminating MOTUs were removed from the dataset. The MOTUs were also filtered to remove all remaining adapter sequences and also retain only those of the expected size (around 170 bp). Finally, taxonomic assignments were made on the MOTUs using the BLAST+ program and the NCBI-nt database. Taxonomic levels (species, genus or family) were assigned using a best match method (Top hit), with a threshold of 95%. Only assignments at the level of fish and marine mammals were considered, and the taxa detected were compared to a list of regional species, and corrected if necessary. The species detections of the different replicas have been combined. The file provided includes generic activity information, including site, station name, date, marker type, assignment types used for taxa identification, and a list of taxa or species. The list of taxa has been verified by a biodiversity expert from the Maurice-Lamontagne Institute. This project was funded by Fisheries and Oceans Canada's Coastal Environmental Baseline Data Program under the Oceans Protection Plan. This initiative aims to acquire baseline environmental data that contributes to the characterization of significant coastal areas and supports evidence-based assessments and management decisions to preserve marine ecosystems. Data were also published on SLGO platform : https://doi.org/10.26071/ogsl-2239bca5-c24a
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Data presented here comes from the 2016 Census of Canada, Statistics Canada. These datasets are part of the Official-Language Minority Communities Dashboard, an initiative of the Research Team of the Official Languages Branch at Canadian Heritage. Four datasets have been organized based on different population groups and universes and explore selected variables such as age, language, socio-economic and socio-demographic characteristics.
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Fisheries and Oceans Canada (DFO) conducted a winter multidisciplinary scientific surveys with a bottom trawl the northern Gulf of St. Lawrence and Cabot Straight from 1978 to 1994. The specificities of the missions onboard the MV Lady Hammond are described below. Objectives: 1. Assess groundfish populations abundance and condition 2. Assess environmental conditions 3. Conduct a biodiversity inventory of benthic and demersal megafauna 4. Collect samples for various research projects Survey description This winter survey covers the northern Gulf of St. Lawrence, that is the divisions 4R, 4S and 3Pn of the Northwest Atlantic Fisheries Organization (NAFO). A stratified random sampling strategy is used for this survey and the fishing gear used on the MV Gadus Atlantica is an Engel 145 Otter trawl. Standard trawling tows last 30 minutes, starting from the time the trawl touches the sea floor. Towing speed is 3.5 knots. Data For each fishing tow, the catch is sorted and weighed by taxa; individuals are counted and biological data are collected on a subsample. For fish, crab and squid, size and weight are measured by individual and, for some species, sex, gonad maturity, and the weight of certain organs (stomach, liver, gonads) are also evaluated. The soft rays of the anal fin are counted for redfish and otoliths are collected for Greenland halibut and Atlantic cod. Invertebrates are weighted and counted (no individual measurements). The biological data are divided into 4 files: a “Metadata” file containing set information, a “Catches” file containing catches per set for fish taxa, a “Carbio” file containing morphometric measurements per individual and a “Freql” file containing the length frequency of fish. It's important to note that this is raw data. Only sets considered successful are retained. In each set, all species are kept, with a few exceptions. For more information please contact the data management team (gddaiss-dmsaisb@dfo-mpo.gc.ca).
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PURPOSE: These data have been updated following a Canadian Science Advice Secretariat (CSAS) Regional Science Advisory Process. Associated publications are available in the citation section below or will be posted on the Fisheries and Oceans Canada (DFO) Science Advisory Schedule as they become available. The dataset supports stock assessment by providing estimates of total returns of large and small Atlantic salmon to each Salmon Fishing Area (SFA) from 15 to 18, as well as to Gulf region rivers overall. DESCRIPTION: This dataset contains annual estimates of total returns of large and small Atlantic salmon to Salmon Fishing Areas (SFAs) 15 to 18 and to Gulf region rivers, as part of the stock assessment process up to the year 2022. USE LIMITATION: To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.
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The objective of this project was to gather data to develop a model of the food web of the lower trophic levels of the nearshore area of the Beaufort Sea. Sampling took place from 2005 to 2008 using the CCGS Nahidik. The multidisciplinary character of the Nahidik program produced measurements of biology/ecology (primary production, phytoplankton, zooplankton, benthos, fish), chemical and physical oceanography, contaminants, geology and hydro acoustics. The data were collected in July and August of each year. The Nahidik program provided data to provide a baseline for future studies as well as an information source for environmental assessment.
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Ichthyoplankton surveys were conducted in the Strait of Georgia (British Columbia) during 1979-1981 to ascertain the onset of fish spawning, and to explore distributional pattern and estimate total biomass of fish species. Oblique tows were made using 0.25m2 Bongos equipped with 351 micron Nitex nets of modified SCOR design. All sampling gear was black to minimize potential avoidance and resulting catch bias. The tow procedure generally followed that established by CALCOFI. This dataset contains a compilation of corrected catches of juvenile fishes, fish eggs and fish larvae by station.
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Fisheries and Oceans Canada (DFO) has been conducting surface water trawl surveys since 1992 in the coastal waters of British Columbia, Washington, Oregon and Alaska and in the high seas of the Gulf of Alaska. These surveys initially focused on determining the migratory patterns (1992-2002) and on the growth and physiology (2003-2016) of juvenile Pacific Salmon. Data collected in shelf and slope waters at depths less than 400 meters off Washington and Oregon State are part of this larger survey series, but are limited to 1999-2001. These surveys focused on determining the migratory patterns of juvenile Pacific Salmon and had funding support from the Bonneville Power Administration as part of the 1995-2011 Canada-USA Salmon Shelf Survival Study. The intent of that study was to monitor and evaluate the effects of ocean conditions on the distribution, migration, growth, and survival of Pacific salmon during their first ocean year, and estimate the subsequent impacts on abundance of Chinook salmon adults returning to the Columbia River system.
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This resource documents a dataset of epifauna occurrences collected in 2021 during The Knowledge and Ecosystem-Based Approach in Baffin Bay (KEBABB) program developed by the Department of Fisheries and Oceans Canada (DFO) in collaboration with university partners. The overall objective of KEBABB is to characterize the variability and trends in physical, chemical, and biological oceanographic conditions and food webs supporting fisheries in the connected ecosystems of western Baffin Bay and Lancaster Sound. In 2021, DFO expanded the KEBABB program to Barrow Strait (KEBABS-Knowledge and Ecosystem-Based Approach in Barrow Strait), a key productive area of the Tallurutiup Imanga National Marine Conservation Area. The study took place in the Eastern Canadian Arctic (mainly in Baffin Bay, Davis Strait and Barrow Strait). Sampling is done along transects at fixed stations in the study area. Catches are collected with a 1.5 m Agassiz trawl (5 mm mesh net) for 3 minutes bottom-contact time at a target speed of 1.5 knots and with a 3 m benthic beam trawl (6.4 mm mesh net) for 15 minutes bottom-contact time at a target speed of 3 knots. A total of 16 stations were sampled for epifauna in 2021 between 85-850 m depth. Epibenthic invertebrates are identified to the lowest possible taxonomic level and photographed. All unknown specimens are frozen. In the lab, the identifications are validated or refined with the photos and the frozen specimens. The data are presented in Darwin Core and are separated in two files: The “Activité_épifaune_KEBABB_epifauna_event_en” file which contains information about missions, stations and deployments, which are presented under a hierarchical activity structure. The “Occurrence_épifaune_KEBABB_epifauna_en” file that contains the taxonomic occurrences. Further details on sampling can be found in the following report: Pućko, M., Charette, J., Tremblay P., Brulotte S., St-Denis B., Ciastek S., Hedges, K., Kuzyk, Z., Roy V., and Michel, C. 2022. An ecosystem-based approach in the eastern Arctic: KEBABB/S (Knowledge and Ecosystem-Based Approach in Baffin Bay/Barrow Strait) 2021 expedition report. Can. Manuscr. Rep. Fish. Aquat. Sci. 3250: viii + 58 p. https://publications.gc.ca/collections/collection_2022/mpo-dfo/Fs97-4-3250-eng.pdf USE LIMITATION: To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.
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PURPOSE: To characterize food webs of lake fish communities using stable isotopes, gut content morphology and DNA. DESCRIPTION: Data sets containing fish species caught in Miramichi Lake, McKiel Lake and Nashwaak Lake. PARAMETERS COLLECTED: Species counts (ecological); points (spatial) USE LIMITATION: To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.
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This record contains results from chemical analysis including suspended nitrogen (mg/g), suspended carbon (mg/g), and phosphorus (mg/g) based on dry weight sediment samples collected in the Beaufort Sea.
Arctic SDI catalogue