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RI_623

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    Concentrations of sea pens, small and large gorgonian corals and sponges on the east coast of Canada have been identified through spatial analysis of research vessel survey by-catch data following an approach used by the Northwest Atlantic Fisheries Organization (NAFO) in the Regulatory Area (NRA) on Flemish Cap and southeast Grand Banks. Kernel density analysis was used to identify high concentrations. These analyses were performed for each of the five biogeographic zones of eastern Canada. The largest sea pen fields were found in the Laurentian Channel as it cuts through the Gulf of St. Lawrence, while large gorgonian coral forests were found in the Eastern Arctic and on the northern Labrador continental slope. Large ball-shaped Geodia spp. sponges were located along the continental slopes north of the Grand Banks, while on the Scotian Shelf a unique population of the large barrel-shaped sponge Vazella pourtalesi was identified. The latitude and longitude marking the positions of all tows which form these and other dense aggregations are provided along with the positions of all tows which captured black coral, a non-aggregating taxon which is long-lived and vulnerable to fishing pressures.

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    A derivative of DFO’s benthic species survey for the Strategic Program for Ecosystem-based Research and Advice (SPERA) (open data record ID: e736c0f0-b19e-4842-903d-28bfc756d48a), this benthic survey funded through the Canadian Healthy Oceans Network (CHONeII) looks at the presence/absence and abundance of two biogenic habitat-forming species that are listed as vulnerable to disturbance in a subset of 50 drift camera transects in the ‘Head Harbour/West Isles Archipelago/The Passages’ Ecologically and Biologically Significant Area (EBSA) in the Bay of Fundy, New Brunswick, Canada (~113km2). Presence/absence and abundance data of the stalked sea squirt (Boltenia ovifera) and horse mussel (Modiolus modiolus) were derived from the use of high-resolution Nikon D800 36.1 megapixel still images (n=2576, see link to parent record for more descriptive survey information) to be used in species distribution modelling. Image field of view (FOV) was estimated using a 10 cm-wide trigger weight for scale,and standardized across images using the average FOV estimate (0.75 x 0.5 m) across a subset of 200 images. Species counts were then converted to abundance estimates (number of individuals per square-meter) by dividing counts by 0.375m2. Boltenia ovifera was observed at densities reaching 456 ind./m2, while Modiolus modiolus density reached a maximum of 240 ind./m2. Cite this data as: Mireault C.A., Lawton P., Devillers R. and Teed L. Presence/absence and abundance of vulnerable marine ecosystem species Boltenia ovifera and Modiolus modiolus in the lower Bay of Fundy derived from high resolution still imagery. Published September 2023. Coastal Ecosystems Science Division, Fisheries and Oceans Canada, St. Andrews, N.B. https://open.canada.ca/data/en/dataset/152ae3f1-d2b9-43d9-a7b4-d769d9e9fc41

  • This release makes available the West Canadian Coast part of the results of an ongoing effort to scan and convert all our inventory of analog marine survey field records (seismic, sidescan and sounder) to digital format. These records have been scanned at 300 dpi and were converted into JPEG2000 format. Typically each of these files were from 1 to 2 gbyte in size before compression, and were compressed by a factor of 10:1. Empirical tests with a number of data sets suggest that there is minimal visual distortion of the scanned data at this level of compression. In this KML file, scanned data are available in a reduced-scale thumbnail format and a compressed full-resolultion JPEG2000 format.

  • An archive of 2D regional seismic and long period magnetotelluric data collected during 20 years of work under the LITHOPROBE project. Data are primarily onshore and cover widespread regions of Canada. Available data types include raw digital data, processed sections, and images of final sections, as well as auxiliary information required for analysis of the data.

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    Frost Day Count (-4 °C) is defined as the count of the number of days in a calendar month where the minimum daily temperature for the climate day was at or below -4 °C. These values are calculated across Canada in 10x10 km cells.

  • Vertical seismic profiling (VSP) surveys done by the Geological Survey of Canada for research into downhole seismic imaging techniques for mineral exploration.

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    Tree swallow (Tachycineta bicolor) nest boxes are installed in oil sands area and in reference locations to monitor contaminant levels and impacts on tree swallow nestlings. The exposure to tree swallow nestlings to air-borne oil sands-related contaminants such as polycyclic aromatic hydrocarbons (PAHs) is assessed using passive air samplers. Measures of avian health are examined in relation to location of sampling and contaminants measured.

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    Zooplankton samples were collected at Ocean Station "P" (50.0000, -145.0000) from 1956 to 1980, and were analyzed to various levels of taxonomic resolution over the years. Although summaries of these data have been previously published ((LeBrasseur 1965) and (Fulton 1978, 1983)) the detailed species data have never been published. This detailed dataset contains total zooplankton wet weights/m3 for the whole period of 1956 to 1980, as well as densities (numbers/m3) for five major taxa (copepods, chaetognaths, euphausiids, amphipods, and Aglantha) from 1964 to 1967, species identifications, counts and lengths for many samples collected between 1968 to 1980. The attached supporting document (Ocean Station "Papa" detailed zooplankton data: 1956 – 1980) contains information on the methods used to collect and process the data along with descriptions of a number of fairly minor points about the data that were not resolved. It also describes, in detail, the format of the original data files, the corrections/changes that were made to these files in creating this version, and how these errors affect what was published in Fulton (1983). The purpose of this record is to make the detailed data available to the scientific community in an electronic format and to provide a convenient reference for citing the detailed data. Waddell, Brenda J., and Skip McKinnell. 1995. Ocean Station "Papa" detailed zooplankton data: 1956 - 1980. Can. Tech. Rep. Fish. Aquat. Sci. 2056: 21 p.

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    Description: Seasonal mean total phytoplankton at the surface from the British Columbia continental margin model (BCCM) were averaged over the 1981 to 2010 period to create seasonal mean surface climatology of the Canadian Pacific Exclusive Economic Zone. Methods: Total phytoplankton is the sum of diatoms and flagellates concentration. Spring months were defined as April to June, summer months were defined as July to September, fall months were defined as October to December, and winter months were defined as January to March. The data available here contain a raster layer of seasonal surface phytoplankton climatology for the Canadian Pacific Exclusive Economic Zone at 3 km spatial resolution. Uncertainties: Model results have been extensively evaluated against observations (e.g. altimetry, CTD and nutrient profiles, observed geostrophic currents), which showed the model can reproduce with reasonable accuracy the main oceanographic features of the region including salient features of the seasonal cycle and the vertical and cross-shore gradient of water properties. However, the model resolution is too coarse to allow for an adequate representation of inlets, nearshore areas, and the Strait of Georgia.

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    Cold-water corals are conspicuous in the waters off Eastern Canada. Despite that, there are few DNA sequence records from specimens collected in the region available in GenBank, and not all species recorded in the region have sequence data regardless of geographic origin. This can limit the use of eDNA techniques to detect and identify corals. Our objective was to sequence and publish sequences for two octocoral DNA barcoding markers: CO1 and MutS. We sequenced and deposited 36 sequences to GenBank from 19 specimens representing three sea pen taxa (Octocorallia: Pennatuloidea): Distichoptilum gracile, Pennatula aculeata, and Protoptilum carpenteri. Identification of all specimens was confirmed by B. M. Neves before submission. Specimens and DNA tissues were donated to the Canadian Museum of Nature, where they are currently stored. This publication is part 1 of a series of GenBank submissions by our lab. Specimens were collected from across the Northwest Atlantic and originate from depths ranging between 200-1924 meters. Specimens were collected as part of research vessel multispecies trawl surveys or remotely operated vehicle (ROV ROPOS) surveys. DNA was isolated and purified using the QIAgen DNeasy Blood and Tissue kit, with an initial overnight incubation with Proteinase K. Two commonly used octocoral barcoding regions were amplified using previously described primers: 1) COII8068F (McFadden et al., 2004) and COIOCTR (France and Hoover, 2002) for the CO1 gene, and 2) ND42599F (France and Hoover, 2002) and mut3458R (Sánchez et al., 2003) for the MutS gene. Amplifications were conducted using 12.5 µl of Green DreamTaq Master Mix (Thermo Fisher Scientific), 1 µl of template DNA, 0.5 µl of each 10 µM forward and reverse primers, 0.5 µl of 10 µM reverse primer, and 10.5 µl of water. Thermocycling was run as follows: 3 min of initial denaturation at 95 °C, followed by 40 cycles at 95 °C for 30 s, 30 s at annealing temperature of 48 °C, then 65 s at an extension temperature of 72 °C, and a final elongation at 72 °C for 4 min. PCR products were cleaned using Agencourt AMPure XP Beads (Beckman Coulter) and sent to The Center for Advanced Genomics, Toronto, Canada for Sanger sequencing. Sequences were visualized and aligned using Geneious Prime 2022.0.2. Obtained sequences have been deposited in GenBank under accession numbers OQ569768- OQ569784 and OQ420359- OQ420377. This work was funded by Fisheries and Oceans Canada under an Enhanced Regional Capacity grant (2020-2021) and the Marine Conservation Targets (MCT) program (2021-2024), Newfoundland and Labrador Region.