Aquatic invasive species (AIS) are non-native species that pose ecological and/or economic threats to Canada's coastal waters and resources. In response, Fisheries and Oceans Canada (DFO) established a program to detect and track the spread of aquatic invasive species in Canadian waters (2006). In the Newfoundland and Labrador (NL) Region, these species include biofouling organisms (tunicates, bryozoans, crustaceans, seaweeds), European Green Crab, and recently, several freshwater species (data not available at this time). DFO NL Science monitors for AIS in partnership with other DFO branches, the provincial government, Memorial University, including the Marine Institute, industry, first nations, and NGOs. Methods for detecting AIS used by DFO and their partners, include settlement plates, trapping, seining, eDNA (water samples), qPCR (species confirmation), SCUBA surveys along wharves, floating docks and vessel hulls, and video surveys at high-risk harbours. The data collected from DFO's monitoring program and their partners provides an overview of the distribution of AIS in the NL Region. This information can be used by the general public, scientists, and DFO managers. AIS NL Biofouling Species Fisheries and Oceans Canada's (DFO) National Marine Biofouling Monitoring Program conducts annual field surveys to monitor the introduction, establishment, spread, species richness, and relative abundance of native and some non-native species in Newfoundland and Labrador (NL) Region since 2006. Standardized monitoring protocols employed by DFO's NL, Maritimes, Gulf, and Quebec regions include biofouling collector plates deployed from May to October at georeferenced intertidal and shallow subtidal sites, including public docks, and public and private marinas and nautical clubs. Initially, (2006-2017), the collectors consisted of three 10 cm by 10 cm PVC plates deployed in a vertical array and spaced approximately 40 cm apart, with the shallowest plate suspended at least 1 m below the surface to sample subtidal and shallow intertidal species (McKenzie et al 2016a). Three replicate arrays were deployed at least 5 m apart per site. Since 2018, collector networks have been modified to improve statistical replication, including up to 10 individual collectors deployed per site at 1 m depth and at least 5 m apart (as above) from May to October. Since 2006, seven invasive biofouling organisms have been detected in Newfoundland and Labrador harbours, marinas and coastal areas. Should be cited as follows: DFO Newfoundland and Labrador Region Aquatic Invasive Species Marine Biofouling Monitoring Program. Published March 2024. Coastal and Freshwater Ecology, Northwest Atlantic Fisheries Centre, Fisheries and Oceans Canada, St. John’s, Newfoundland and Labrador. Reference: Tunicates Golden star tunicate (Botryllus schlosseri) 2006 The Golden star tunicate was the first invasive tunicate detected in NL waters. It was reported in Argentia by the US Navy around 1945. It was found in 2006 on wharf structures in Argentia, Placentia Bay during the first AIS survey (Callahan et al 2010). This colonial tunicate is recognized by it star shaped grouping of individuals within the colony. It is currently found in Placentia Bay, Fortune Bay, St. Mary’s Bay, Conception Bay and the west coast of NL. The data provided here indicates the detections of this AIS in coastal NL. Violet tunicate (Botrylloides violaceus) 2007 The violet tunicate was first detected in NL waters in 2007 in Belleoram, Fortune Bay on wharf structures and vessels (McKenzie et al. 2016b). This colonial tunicate forms irregular shaped colonies usually of a solid color (orange, purple, yellow or cream). It is currently found in relatively small colonies in four harbours in NL; Placentia Bay (1), Fortune Bay (1), Conception Bay (1) and the west coast of NL (2). The data provided here indicates the detections of this AIS in coastal NL. Vase tunicate (Ciona intestinalis) 2012 The Vase tunicate, is a high impact solitary invader and was first detected by DFO in 2012 on the Burin Peninsula at Ship Cove and Little Bay, Placentia Bay. Various mitigation measures (McKenzie et al. 2016b) contained this invasive tunicate to a small area for six years within Placentia Bay. First detected in Fortune Bay as an established population in 2019, increasing reports of the Vase tunicate have been made along the south coast of Newfoundland. The data provided here indicates the detections of this AIS in coastal NL. Bryozoans Coffin box bryozoan (Membranipora membranacea) 2002 The Coffin box is a bryozoan (filter feeding animal) that forms white colored encrusting colonies, particularly on seaweed, but also on vessels and other surfaces. The cells are rectangular or “coffin box” shaped. It was first detected on the west coast of NL in 2002 and has since spread throughout the island, including southern Labrador. The data provided here indicates the detections of this AIS in coastal NL. Orange ripple bryozoan (Schizoporella japonica Ortmann) 2022 The Orange ripple bryozoan is an orange heavily calcified encrusting species found mainly on man made structures, rocks, shellfish, and vessels. It was first identified in NL in 2022 in Arnold’s Cove, Placentia Bay, but has likely been in NL for at least two years and is now suspected in several locations in Placentia Bay and Fortune Bay. A 2023 survey of the south coast of NL found several harbours invaded by this species. The data provided here indicates the detections of this AIS in coastal NL. Crustaceans (Biofouling) Japanese skeleton shrimp (Caprella mutica) 2006 The Japanese skeleton shrimp is tiny (1.5 -3.5 cm) and was first found on settlement plates in Placentia Bay during the first AIS biofouling survey in 2006. This species is currently found in many places in Placentia Bay, Conception Bay, Trinity Bay, and on the south coast of NL. This species inhabits, sometimes in large numbers (100,000s) ropes, moorings, and docks. The data provided here indicates the detections of this AIS in coastal NL. Seaweed Oyster thief (Codium fragile) 2012 Oyster thief is a green seaweed with thick spongy Y-shaped branches that resemble fingers (another common name is dead man’s fingers). This invasive species was first found in Placentia Bay in 2012. It is now found in several locations in Placentia Bay and Fortune Bay. It has also been found in one location in Notre Dame Bay. The data provided here indicates the detections of this AIS in coastal NL. AIS NL European green crab Fisheries and Oceans Canada's (DFO) AIS Science Program conducts annual field surveys to monitor the introduction, establishment, spread, and relative abundance of the European green crab in the NL region since 2006. Standardized monitoring and trapping protocols (McKenzie et al. 2022) are used by DFO's NL, Maritimes, Gulf, Quebec, and Pacific regions. The Fukui trap is the most commonly used trap but other methods are also used including shoreline collection, seining, and SCUBA dive surveys. Fukui traps have been deployed annually at both new and long-term monitoring locations throughout coastal Newfoundland and Labrador, particularly within Placentia Bay where they were first detected in North Harbour in 2007 (Blakeslee et al. 2010). It should be noted that there are two different populations of European Green Crab, a hybridized population in Placentia, St. Mary’s, and Fortune Bays and a cold tolerant population on the west and southwestern coast of NL (Lehnert et al. 2018). After initial detection in 2007, a separate invasion occurred in 2009 on the west coast of NL, and this cold-tolerant population is spreading eastward along the south coast toward areas inhabited by the original hybrid strain. The data provided here indicates the detections of this AIS in coastal NL. Should be cited as follows: DFO Newfoundland and Labrador Region Aquatic Invasive Species Green Crab Monitoring Program. Published March 2024. Coastal and Freshwater Ecology, Northwest Atlantic Fisheries Centre, Fisheries and Oceans Canada, St. John’s, Newfoundland and Labrador. From 2018-2022, the Coastal Environmental Baseline Program provided additional support to enhance sampling efforts in Placentia Bay.

The land division system used for describing the extent of oil and gas interests located in the Northwest Territories, Nunavut or in Canada's offshore area is defined in the Canada Oil and Gas Land Regulations. This land division system consists of a grid system divided into Grid Areas, Sections, and Units – all referenced to the North American Datum of 1927 (NAD27). This data provides the geo-spatial representation of the NAD27 Oil and Gas Grid Areas referenced to NAD83 Datum. The creation of the Oil and Gas Grid Areas geo-spatial file covers areas that are situated in the Northwest Territories, Nunavut or Sable Island as well as submarine areas, not within a province, in the internal waters of Canada, the territorial sea of Canada or the continental shelf of Canada beyond 200 nm zone. The NAD83 grid area boundaries are defined by geodesics joining the four grid area corners. For sections and units, the eastern and western grid area geodesic boundaries are partitioned into 40 equal segments. The northern and southern grid area geodesic boundaries are partitioned into 40, 32 or 24 equal segments, depending on latitude. All internal corners at the section and unit level are defined by the intersections of north-south and east-west geodesics joining corresponding partition points along the northern and southern, and eastern and western, grid area geodesic boundaries.

AIS NL Biofouling Species Fisheries and Oceans Canada's (DFO) National Marine Biofouling Monitoring Program conducts annual field surveys to monitor the introduction, establishment, spread, species richness, and relative abundance of native and some non-native species in Newfoundland and Labrador (NL) Region since 2006. Standardized monitoring protocols employed by DFO's NL, Maritimes, Gulf, and Quebec regions include biofouling collector plates deployed from May to October at georeferenced intertidal and shallow subtidal sites, including public docks, and public and private marinas and nautical clubs. Initially, (2006-2017), the collectors consisted of three 10 cm by 10 cm PVC plates deployed in a vertical array and spaced approximately 40 cm apart, with the shallowest plate suspended at least 1 m below the surface to sample subtidal and shallow intertidal species (McKenzie et al 2016a). Three replicate arrays were deployed at least 5 m apart per site. Since 2018, collector networks have been modified to improve statistical replication, including up to 10 individual collectors deployed per site at 1 m depth and at least 5 m apart (as above) from May to October. Since 2006, seven invasive biofouling organisms have been detected in Newfoundland and Labrador harbours, marinas and coastal areas. Should be cited as follows: DFO Newfoundland and Labrador Region Aquatic Invasive Species Marine Biofouling Monitoring Program. Published March 2024. Coastal and Freshwater Ecology, Northwest Atlantic Fisheries Centre, Fisheries and Oceans Canada, St. John’s, Newfoundland and Labrador. Reference: Tunicates Golden star tunicate (Botryllus schlosseri) 2006 The Golden star tunicate was the first invasive tunicate detected in NL waters. It was reported in Argentia by the US Navy around 1945. It was found in 2006 on wharf structures in Argentia, Placentia Bay during the first AIS survey (Callahan et al 2010). This colonial tunicate is recognized by it star shaped grouping of individuals within the colony. It is currently found in Placentia Bay, Fortune Bay, St. Mary’s Bay, Conception Bay and the west coast of NL. The data provided here indicates the detections of this AIS in coastal NL. From 2018-2022, the Coastal Environmental Baseline Program provided additional support to enhance sampling efforts in Placentia Bay.

Many cetacean species were depleted in Canadian Pacific waters by commercial whaling, which ended in 1967. Although some populations have since shown evidence of recovery, there is limited information about the current abundance and geographic distribution of many species, particularly in difficult-to-survey offshore regions. This lack of baseline data hampers conservation status assessments, including estimating population-level impacts of anthropogenic activities. From July to early September 2018, we conducted ship-based surveys of cetaceans throughout the coastal and offshore waters of British Columbia. Density surface modelling (DSM) was used to produce spatially-explicit abundance estimates and distribution maps for four commonly-encountered cetacean species: the humpback whale (Megaptera novaeangliae), fin whale (Balaenoptera physalus), Dall’s porpoise (Phocoenoides dalli) and harbour porpoise (Phocoena phocoena). We estimated abundances of 7,030 (95% CI = 5,733-8,620) humpback whales, 2,893 (95% CI = 2,171-3,855) fin whales, 23,692 (95% CI = 19,121-29,356) Dall’s porpoises and 5,207 (95% CI = 2,769-9,793) harbour porpoises throughout Canadian Pacific waters. Our results complement design-based abundance estimates calculated from the same survey data, and can be compared with past habitat modelling studies and historical whaling catch data to estimate the extent of recovery of previously harvested populations. The return of these predators to habitats from which they were previously extirpated will have important ecosystem-level implications. The DSM results can contribute to calculations of Potential Biological Removal estimates to inform fisheries bycatch, as well as providing spatial data that can be used to assess the risk of entanglements, ship strikes, acoustic disturbance, and other anthropogenic threats. This dataset contains model-predicted densities of four commonly-encountered cetacean species (humpback whale, fin whale, Dall's porpoise and harbour porpoise) that were estimated using ship-based, visual survey data collected during the Pacific Region International Survey of Marine Megafauna (PRISMM) in July-August of 2018. Abundance of each species (where relevant) is provided for three gridded strata (25 km2 cell size) in the Pacific Region: one for the offshore, extending to Canada’s exclusive economic zone (EEZ), and two for coastal areas (the North Coast and the Salish Sea).

AIS NL Biofouling Species Fisheries and Oceans Canada's (DFO) National Marine Biofouling Monitoring Program conducts annual field surveys to monitor the introduction, establishment, spread, species richness, and relative abundance of native and some non-native species in Newfoundland and Labrador (NL) Region since 2006. Standardized monitoring protocols employed by DFO's NL, Maritimes, Gulf, and Quebec regions include biofouling collector plates deployed from May to October at georeferenced intertidal and shallow subtidal sites, including public docks, and public and private marinas and nautical clubs. Initially, (2006-2017), the collectors consisted of three 10 cm by 10 cm PVC plates deployed in a vertical array and spaced approximately 40 cm apart, with the shallowest plate suspended at least 1 m below the surface to sample subtidal and shallow intertidal species (McKenzie et al 2016a). Three replicate arrays were deployed at least 5 m apart per site. Since 2018, collector networks have been modified to improve statistical replication, including up to 10 individual collectors deployed per site at 1 m depth and at least 5 m apart (as above) from May to October. Since 2006, seven invasive biofouling organisms have been detected in Newfoundland and Labrador harbours, marinas and coastal areas. Should be cited as follows: DFO Newfoundland and Labrador Region Aquatic Invasive Species Marine Biofouling Monitoring Program. Published March 2024. Coastal and Freshwater Ecology, Northwest Atlantic Fisheries Centre, Fisheries and Oceans Canada, St. John’s, Newfoundland and Labrador. Reference: Tunicates Vase tunicate (Ciona intestinalis) 2012 The Vase tunicate, is a high impact solitary invader and was first detected by DFO in 2012 on the Burin Peninsula at Ship Cove and Little Bay, Placentia Bay. Various mitigation measures (McKenzie et al. 2016b) contained this invasive tunicate to a small area for six years within Placentia Bay. First detected in Fortune Bay as an established population in 2019, increasing reports of the Vase tunicate have been made along the south coast of Newfoundland. The data provided here indicates the detections of this AIS in coastal NL. From 2018-2022, the Coastal Environmental Baseline Program provided additional support to enhance sampling efforts in Placentia Bay.

This project was completed by the Coastal Environmental Baseline Program (Coastal and Freshwater Ecology Section) in the Newfoundland and Labrador Science Branch of Fisheries and Oceans Canada (DFO). From 2020-2023, there were semi-annual deployments and retrievals of 3 moored CTDs in the bay. From 2020 to November 2021, moored CTDs collected hourly recordings of conductivity, temperature and pressure. From 2021 through 2023, these CTDs collected year-round, hourly, information on temperature, conductivity, pressure, dissolved oxygen, and chlorophyll-a. Acoustic releases also collected hourly ambient noise (mV) data. This record contains the geographic locations of the sites, and information on the timings and types of data collected at each site.

Data Sources: Banque informatisée des oiseaux de mer au Québec (BIOMQ: ECCC-CWS Quebec Region) Atlantic Colonial Waterbird Database (ACWD: ECCC-CWS Atlantic Region).. Both the BIOMQ and ACWD contain records of individual colony counts, by species, for known colonies located in Eastern Canada. Although some colonies are censused annually, most are visited much less frequently. Methods used to derive colony population estimates vary markedly among colonies and among species. For example, census methods devised for burrow-nesting alcids typically rely on ground survey techniques. As such, they tend to be restricted to relatively few colonies. In contrast, censuses of large gull or tern colonies, which are geographically widespread, more appropriately rely on a combination of broad-scale aerial surveys, and ground surveys at a subset of these colonies. In some instances, ground surveys of certain species are not available throughout the study area. In such cases, consideration of other sources, including aerial surveys, may be appropriate. For example,data stemming from a 2006 aerial survey of Common Eiders during nesting, conducted by ECCC-CWS in Labrador, though not yet incorporated in the ACWD, were used in this report. It is important to note that colony data for some species, such as herons, are not well represented in these ECCC-CWS databases at present. Analysis of ACWD and BIOMQ data (ECCC-CWS Quebec and Atlantic Regions): Data were merged as temporal coverage, survey methods and geospatial information were comparable. Only in cases where total counts of individuals were not explicitly presented was it necessary to calculate proxies of total counts of breeding individuals (e.g., by doubling numbers of breeding pairs or of active nests). Though these approaches may underestimate the true number of total individuals associated with a given site by failing to include some proportion of the non-breeding population (i.e., visiting adult non-breeders, sub-adults and failed breeders), tracking numbers of breeding individuals (or pairs) is considered to be the primary focus of these colony monitoring programs.In order to represent the potential number of individuals of a given species that realistically could be and may historically have been present at a given colony location (see section 1.1), the maximum total count obtained per species per site since 1960 was used in the analyses. In the case of certain species,especially coastal piscivores (Wires et al. 2001; Cotter et al. 2012), maxima reached in the 1970s or 1980s likely resulted from considerable anthropogenic sources of food, and these levels may never be seen again. The effect may have been more pronounced in certain geographic areas. Certain sites once used as colonies may no longer be suitable for breeding due to natural and/or human causes, but others similarly may become suitable and thus merit consideration in long-term habitat conservation planning. A colony importance index (CII) was derived by dividing the latter maximum total count by the potential total Eastern Canadian breeding population of that species (the sum of maximum total counts within a species, across all known colony sites in Eastern Canada). The CII approximates the proportion of the total potential Eastern Canadian breeding population (sum of maxima) reached at each colony location and allowed for an objective comparison among colonies both within and across species. In some less-frequently visited colonies, birds (cormorants, gulls, murres and terns, in particular) were not identified to species. Due to potential biases and issues pertaining to inclusion of these data, they were not considered when calculating species’ maximum counts by colony for the CII. The IBA approach whereby maximum colony counts are divided by the size of the corresponding actual estimated population for each species (see Table 3.1.2; approximate 1% continental threshold presented) was not used because in some instances individuals were not identified to species at some sites, or population estimates were unavailable.Use of both maxima and proportions of populations (or an index thereof) presents contrasting, but complementary, approaches to identifying important colonial congregations. By examining results derived from both approaches, attention can be directed at areas that not only host large numbers of individuals, but also important proportions of populations. This dual approach avoids attributing disproportionate attention to species that by their very nature occur in very large colonies (e.g., Leach’s Storm Petrel) or conversely to colonies that host important large proportions of less-abundant species (Roseate Tern, Caspian Tern, Black-Headed Gull, etc.), but in smaller overall numbers. Point Density Analysis (ArcGIS Spatial Analyst) with kernel estimation, and a 10-km search radius,was used to generate maps illustrating the density of colony measures (i.e., maximum count by species,CII by species), modelled as a continuous field (Gatrell et al. 1996). Actual colony locations were subsequently overlaid on the resulting cluster map. Sites not identified as important should not be assumed to be unimportant.

This project was completed by the Shellfish Section in the Newfoundland and Labrador Science Branch of Fisheries and Oceans Canada (DFO), in collaboration with industry partners. The Coastal Environmental Baseline program supported the Placentia Bay portion of project work for an ongoing industry-DFO collaborative post-season trap survey for Snow Crab that was initiated in 2003 and has occurred each year. This survey is conducted by Snow Crab harvesters accompanied by at-sea observers and takes place in NAFO Divisions 2J3KLNOP4R. Historically the survey focused on commercial fishing grounds but began transitioning to a partly random stratified design in 2017. Since 2018, approximately 50% of survey stations are randomly allocated while 50% remain fixed. At each station, six (for inshore stations) or ten (for offshore stations) commercial traps are set in a fleet. To gather data on non-commercial sized Snow Crab, including females, many fleets also include one small-mesh trap. The coverage of small-mesh traps has been expanding in recent years with the aim of one small-mesh trap for every station in the coming years. Biological sampling is undertaken on at least one commercial trap and the small-mesh trap at each station. The data from this survey is incorporated into the annual stock assessment for Snow Crab in the Newfoundland and Labrador region. This record contains trap locations for Placentia Bay, and information on the types of data collected. More detailed information can be found in Pantin et al. (2022). https://publications.gc.ca/collections/collection_2023/mpo-dfo/fs70-5/Fs70-5-2022-076-eng.pdf

Herring Section shapefile - used for spatial analysis/presentation of data from Herring Stock Assessment Database.

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